WNKs and Sodium Transporter Function in Solute Exchange with Implications for Blood Pressure Regulation as Elucidated through Drosophila DOI Creative Commons
Aylin R. Rodan

Kidney360, Journal Year: 2024, Volume and Issue: 5(10), P. 1553 - 1562

Published: Aug. 26, 2024

Like other multicellular organisms, the fruit fly Drosophila melanogaster must maintain homeostasis of internal milieu, including maintenance constant ion and water concentrations. In mammals, with no lysine (K) (WNK)-Ste20-proline/alanine rich kinase/oxidative stress response 1 kinase cascade is an important regulator epithelial transport in kidney. This pathway regulates SLC12 family cotransporters, sodium-potassium-2-chloride, sodium chloride, potassium chloride cotransporters. The WNK-Ste20-proline/alanine also via regulation sodium-potassium-2-chloride cotransporter Malpighian tubule, renal epithelium fly. Studies have contributed to understanding multiple regulators WNK signaling, intracellular potassium, scaffold protein Mo25, hypertonic stress, hydrostatic pressure, macromolecular crowding. These will be discussed together, implications for mammalian kidney function BP control.

Language: Английский

Function and regulation of the insect NaCCC2 sodium transport proteins DOI Creative Commons

Ryan S. Yarcusko,

Maria Hemmi Song,

Grace C. Neuger

et al.

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology, Journal Year: 2024, Volume and Issue: 296, P. 111685 - 111685

Published: June 22, 2024

NaCCC2 transport proteins, including those from Drosophila melanogaster (Ncc83) and Aedes aegypti (aeCCC2), are an insect-specific clade with sequence similarity to Na+-K+-2Cl− cotransporters. Whereas the cotransporters other cation-chloride electroneutral, recent work indicates that Ncc83 aeCCC2 carry charge across membranes. Here, we further characterize regulation properties of expressed in Xenopus oocytes. In cation uptake experiments, Li+ was used as a tracer for Na+ Rb+ K+. oocytes expressing either or greater than water-injected controls, activated by hypotonic swelling, not inhibited ouabain ethyl cinnamate. different water injected controls. Ncc83, plateaued increasing concentrations, apparent Km values range 10 20 mM. Following exposure ouabain, intracellular [Na+] Elevating cAMP (via 8-bromo-cAMP) significantly stimulated both membrane conductances. did affect uptake, but Overall, these results confirm NaCCC2s resemble their some properties. However, unlike cotransporters, they

Language: Английский

Citations

2
Physiology in Perspective DOI

Nikki Forrester

Physiology, Journal Year: 2024, Volume and Issue: 39(3), P. 128 - 128

Published: March 29, 2024

Language: Английский

Citations

0
WNKs and Sodium Transporter Function in Solute Exchange with Implications for Blood Pressure Regulation as Elucidated through Drosophila DOI Creative Commons
Aylin R. Rodan

Kidney360, Journal Year: 2024, Volume and Issue: 5(10), P. 1553 - 1562

Published: Aug. 26, 2024

Like other multicellular organisms, the fruit fly Drosophila melanogaster must maintain homeostasis of internal milieu, including maintenance constant ion and water concentrations. In mammals, with no lysine (K) (WNK)-Ste20-proline/alanine rich kinase/oxidative stress response 1 kinase cascade is an important regulator epithelial transport in kidney. This pathway regulates SLC12 family cotransporters, sodium-potassium-2-chloride, sodium chloride, potassium chloride cotransporters. The WNK-Ste20-proline/alanine also via regulation sodium-potassium-2-chloride cotransporter Malpighian tubule, renal epithelium fly. Studies have contributed to understanding multiple regulators WNK signaling, intracellular potassium, scaffold protein Mo25, hypertonic stress, hydrostatic pressure, macromolecular crowding. These will be discussed together, implications for mammalian kidney function BP control.

Language: Английский

Citations

0