Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: Feb. 3, 2025
Introduction The diagnosis of Sjögren’s disease (SjD) in patients without autoantibodies against Ro/SSA is a major challenge. We aimed to identify novel SjD that may facilitate the diagnostic procedure for negative SjD. Methods IgG and IgA autoantibody reactivity 94 potential candidate autoantigens SjD, selected from discovery screen 1,629 human antigens coupled Luminex beads prior knowledge about biological relevance, were examined serum (n=347) using ELISA technology. Healthy (HC, n=118) non-Sjögren’s sicca syndrome (NSS, n=44) individuals served as controls. To assess specificity, also measured with Rheumatoid Arthritis (RA, n=50), Systemic Lupus Erythematosus (SLE, n=49), Sclerosis (SSc, n=37). Results 45 significantly (p ≤ 0.05) more prevalent than HC detected up 19% cohort. most common CCL4, M5, TMPO OAS3. Some associated extraglandular manifestations, such anti-TONSL or anti-IL6 pulmonary involvement. have developed three five marker panel detection patients, consisting anti-FNBP4, anti-SNRPC, anti-CCL4, anti-M3 anti-KDM6B, which had sensitivity 46% specificity 95% (SjD vs. HC). Both panels discriminate these HC, whereas three-marker effectively differentiates between NSS. Discussion Novel will particular our predictive be useful differentiation healthy NSS clinical context. In addition, risk stratification manifestations.
Language: Английский
Citations
0Updating on the Dual Role of Salivary Gland Epithelial Cell (SGEC) in Sjögren’s Disease
Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 3039 - 3053
Published: Feb. 28, 2025
Sjögren's disease, an autoimmune inflammatory currently lacks effective treatment options. The salivary gland, a crucial exocrine organ responsible for saliva production and local immune responses on mucous membranes, is frequently impaired in individuals with disease. Restoring gland function poses significant challenge researchers. Salivary epithelial cells, recognized as pivotal components of the have been increasingly implicated key initiators inflammation exhibit innate cell-like properties. On whole, SGEC plays protective role physiological state, can also participate persistence initiating factor pathological state. In review, we explore interplay between Ca+, endoplasmic reticulum (ER), mitochondrial homeostasis imbalance cells. Additionally, provide overview current literature research advancements related to Pattern Recognition Receptors (PRRs), programmed cell death, posttranslational modification (PTM), oral microecology, etc. specifically focusing their implications Given cells onset based may potential alleviate condition by addressing response glands.
Language: Английский
Citations
0A Flexible Multi-Ion Detection System Based on Organic Electrochemical Transistors for Physiological Monitoring
Electronics, Journal Year: 2025, Volume and Issue: 14(5), P. 1023 - 1023
Published: March 4, 2025
The continuous and real-time monitoring of physiological indicators is essential for early disease detection, prevention, clinical diagnosis. In response to the growing demand precise parameter assessment, this study presents a flexible, organic electrochemical transistor (OECT)-based multi-ion sensing system designed monitor key electrolyte concentrations—sodium (Na+), potassium (K+), calcium (Ca2+)—in human biofluids. features highly adaptable sensor array with detection range tailored conditions, ensuring high selectivity stability in complex biological environments. Our demonstrated sensitivity exceeding 1 mA/decade. To enhance measurement accuracy mitigate cross-interference among ions, we integrate advanced machine learning algorithms, which optimize signal processing significantly improve system’s reliability. Additionally, have developed fully integrated hardware–software platform comprising customized acquisition circuitry dedicated data analysis software, specifically OECT-based applications. This comprehensive framework not only refines ion but also paves way broader translation OECT technology. proposed holds great promise point-of-care diagnostics, offering potential paradigm shift non-invasive, on-demand health assessment.
Language: Английский
Citations
0Preventive Effect of Metformin in Radiation‐Induced Xerostomia
Advanced Biology, Journal Year: 2025, Volume and Issue: unknown
Published: March 19, 2025
Abstract Radiation induced structural damage of salivary gland including reducing acinar cell and fibrosis. These changes result in hypofunction which has a significant impact on the quality life head neck cancer patients who treated with radiotherapy. Nevertheless, no preventive method been found. Metformin, diabetes drug, recently attracted considerable attention because it can cause tissue regeneration by anti‐inflammatory action influencing stem cells. This study examined effects metformin caused radiation therapy. Cultured human parotid epithelial cells are irradiated 15 Gy 4MV X‐rays. The analysis DNA damage, inflammatory markers proliferation, is conducted to confirm effect metformin. Similarly, an vivo mouse model established. Histologic functional analyses, such as flow rate lag time, performed. vitro experiment revealed irradiation increased NF‐кB, IL‐6, apoptosis reduced proliferation. treatment decreased radiation‐induced inflammation, also shows same results. group taking after preserved parenchyma compared only group, results comparable normal group. that gland. Hence, be used prevent dysfunction.
Language: Английский
Citations
0Functionalized screen-printed electrodes for non-invasive detection of vascular-endothelial cadherin in extracellular vesicles
RSC Advances, Journal Year: 2025, Volume and Issue: 15(16), P. 12609 - 12621
Published: Jan. 1, 2025
Detection of endothelial biomarker vascular-endothelial cadherin (CD144) on saliva-derived extracellular vesicles using a gold screen-printed electrode biosensor functionalized with mercaptoundecanoic acid and anti-CD144 antibody.
Language: Английский
Citations
0Salivary EGF: EGFR ratio as potential early diagnostic biomarker for oral cancer detection in tobacco chewers: a preliminary cross-sectional study
BMC Oral Health, Journal Year: 2025, Volume and Issue: 25(1)
Published: April 29, 2025
Tobacco chewing and smoking are major risk factors for oral cancer, with Oral Squamous Cell Carcinoma (OSCC), comprising approximately 90% of cases, especially in South-Central Asia. This study examines the early diagnostic value salivary Epidermal Growth Factor (EGF) Receptor (EGFR) OSCC long-term chewers tobacco. assessed EGF EGFR concentrations by Enzyme-Linked Immunosorbent Assay (ELISA), three groups: Healthy Controls (HC, n = 41), Chewers a 10-years history tobacco use (TC, newly diagnosed patients (with more than 10 years) no prior neoplasms or anticancer treatment (OSCC, 19). All participants were over age 35. Salivary levels significantly reduced, while elevated (TC) compared to healthy controls (HC), significant difference observed between TC groups. Notably, EGF: ratio demonstrated better performance alone. ROC analysis highlighted potential these biomarkers as tools TC. may serve an biomarker Reduced suggest their role disease progression, warranting further large-scale studies clinical validation.
Language: Английский
Citations
0High-Resolution Transcriptomic Landscape of the Human Submandibular Gland
Journal of Dental Research, Journal Year: 2023, Volume and Issue: 102(5), P. 525 - 535
Published: Feb. 1, 2023
Saliva-secreting and transporting cells are part of the complex cellular milieu human salivary gland, where they play important roles in normal glandular physiology diseased states. However, comprehensive molecular characterization, particularly at single-cell resolution, is still incomplete, due to difficulty procuring tissues. Here, we perform an in-depth analysis male female adult submandibular gland (SMG) samples by bulk RNA sequencing (RNA-seq) examine underpinnings heterogeneous cell populations (sc) RNA-seq. Our results from scRNA-seq highlight remarkable diversity clusters epithelial nonepithelial that reside SMG also faithfully recapitulated deconvolution bulk-RNA data sets. analyses reveal transcriptomic heterogeneity within both ductal acinar subpopulations identify atypical types, such as mucoacinar unique humans ionocytes have been recently described mouse. We use CellChat explore ligand–receptor interactome predictions likely mediate crucial cell–cell communications between various clusters. Finally, apply a trajectory inference method investigate specific branching points topology offers insights into dynamic differentiation process SMG. The sets herein comprise extensive wealth high-resolution information valuable resource for deeper mechanistic understanding biology pathophysiology.
Language: Английский
Citations
10FGFR2 is essential for salivary gland duct homeostasis and MAPK-dependent seromucous acinar cell differentiation
Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)
Published: Oct. 14, 2023
Abstract Exocrine acinar cells in salivary glands (SG) are critical for oral health and loss of functional is a major clinical challenge. Fibroblast growth factor receptors (FGFR) essential early development multiple organs, including SG. However, the role FGFR signaling specific populations later during differentiation unknown. Here, we use scRNAseq conditional deletion murine FGFRs vivo to identify roles craniofacial, SG progenitor function duct homeostasis. Importantly, also discover that FGFR2 via MAPK seromucous secretory gene expression, while FGFR1 dispensable. We show FGF7, expressed by myoepithelial (MEC), activates FGFR2-dependent transcriptional program. propose model where MEC-derived FGF7 drives differentiation, providing rationale targeting regenerative therapies restore function.
Language: Английский
Citations
10Bioengineered Salivary Gland Microtissues─A Review of 3D Cellular Models and their Applications
ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(5), P. 2620 - 2636
Published: April 9, 2024
Salivary glands (SGs) play a vital role in maintaining oral health through the production and release of saliva. Injury to SGs can lead gland hypofunction decrease saliva secretion manifesting as xerostomia. While symptomatic treatments for xerostomia exist, effective permanent solutions are still lacking, emphasizing need innovative approaches. Significant progress has been made field three-dimensional (3D) SG bioengineering applications regeneration. This achieved major focus on cell culture techniques, including soluble cues biomaterial components 3D niche. Cells derived from both adult embryonic have highlighted key vitro characteristics models. its first decade exploration, spheroids organoids so far served crucial tools study pathophysiology. review, based literature search over past decade, covers importance types realm their isolation, sourcing, conditions that modulate microenvironment. We discuss different biomaterials employed current advances models using them. The success these cellular further evaluated context organ transplantation disease modeling.
Language: Английский
Citations
3Analysis of Histochemical Characteristics of Submandibular Gland of the Bactrian Camel
Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(2), P. 108 - 108
Published: Feb. 2, 2025
The ultrastructure of submandibular gland (SMG) Bactrian camels was observed by a transmission electron microscope. Routine HE staining, special staining combined with immunohistochemistry, and immunofluorescence techniques were used to study the histochemical characteristics localisation distribution epidermal growth factor (EGF) receptor (EGFR). results showed that composed mixed serous mucinous acini glands. striated duct highly developed connected intercalated ducts larger diameter. Intercalated are shorter directly acini. In AB-PAS it inner wall tube strongly positive for AB staining. reticular fibres around follicles is distinct, collagen distributed mainly in periphery sparse Immunohistochemistry fluorescence show EGF ducts, EGFR weakly ducts. camel SMGs secrete more acidic mucins, secreted play role pipeline system SMGs.
Language: Английский
Citations
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