Infertility in Men: Advances towards a Comprehensive and Integrative Strategy for Precision Theranostics

Cells, Journal Year: 2022, Volume and Issue: 11(10), P. 1711 - 1711

Published: May 22, 2022

Male infertility is an increasing and serious medical concern, though the mechanism remains poorly understood. Impaired male reproductive function affects approximately half of infertile couples worldwide. Multiple factors related to environment, genetics, age, comorbidities have been associated with impaired sperm function. Present-day clinicians rely primarily on standard semen analysis diagnose potential develop treatment strategies. To address quality assessment bias enhance accuracy, World Health Organization (WHO) has recommended standardized testing; however, conventional diagnostic therapeutic options for infertility, including physical examination analysis, remain ineffective in relieving social burden. Instead, assisted techniques are becoming primary approach. In post-genomic era, multiomics technologies that deeply interrogate genome, transcriptome, proteome, and/or epigenome, even at single-cell level, besides breakthroughs robotic surgery, stem cell therapy, big data, offer promises towards solving deterioration factor infertility. This review highlights complex etiology especially roles lifestyle environmental factors, discusses advanced technologies/methodologies used characterizing its pathophysiology. A comprehensive combination these innovative approaches a global multi-centric setting fulfilling suitable ethical consent could ensure optimal developmental outcomes. These combinatorial should allow development markers, molecular stratification classes, personalized Since choices influence fertility, their integration any approach required safe, proactive, cost-effective, noninvasive precision theranostics affordable, accessible, facilitate realizing procreation dream.

Language: Английский

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Green Tea Extract in the Extender Improved the Post-Thawed Semen Quality and Decreased Amino Acid Mutation of Kacang Buck Sperm

Veterinary Sciences, Journal Year: 2022, Volume and Issue: 9(8), P. 403 - 403

Published: Aug. 1, 2022

This study was the first to combine addition of antioxidants a skim milk-egg yolk (SM-EY) extender and different equilibration periods obtain higher quality post-thawed Kacang buck semen. aimed determine effects green tea extract (GTE) on frozen goat sperm equilibrated for one two hours. The pool ejaculate equally divided into four portions diluted in an SM-EY that contained doses 0, 0.05, 0.10, 0.15 mg GTE/100 mL T0, T1, T2, T3 groups, respectively. aliquots were treated period 1-2 h before further processing as Post-thawed semen evaluated quality. Sanger method used DNA sequencing, amino acid sequence read using MEGA v.7.0. T2 group hour had highest Pre-freezing motility determination coefficient compared motility. is report mutation due freeze-thawing. frequency mutations revealed least mutated acid. Glycine, valine, leucine, serine, asparagine strongly correlated It can be concluded combination 0.1 antioxidant one-hour resulted best

Language: Английский

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Molecular and physiochemical evaluation of buck semen cryopreserved with antioxidants

Reproduction in Domestic Animals, Journal Year: 2023, Volume and Issue: 58(6), P. 813 - 822

Published: April 4, 2023

Abstract The current study evaluated the physiochemical quality and gene expression profile of post‐thawed buck semen after supplementation with antioxidants [melatonin (M), L‐carnitine (LC), cysteine (Cys), LC + M, M Cys, Cys M] in comparison non‐treated control group. Physical biochemical characteristics were following freezing thawing. Transcript abundance six selected candidate genes was using quantitative real‐time PCR. data demonstrated significant enhancement post‐freezing total motility, progressive percentage live sperm, CASA parameters, plasma membrane acrosome integrity all groups supplemented LC, compared analysis indicated that recorded increased levels GPX SOD coupled up‐regulation antioxidant (SOD1, GPX1 NRF2) mitochondrial transcripts (CPT2 ATP5F1A). Moreover, H2O2 level DNA fragmentation reduced other groups. In conclusion, alone or combination positively improved post‐thaw properties rabbit through activation bioenergetics‐related cellular defence mechanism.

Language: Английский

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Güncel Üroloji Çalışmaları V

Published: April 30, 2024

Ürogenital Sistem Anatomisi Ertürk ALTUN Doğan Sabri TOK Sistemin Endokrin Kontrolü Ridell LOGLI Eray HASIRCI Testiküler Perfüzyonun Radyonüklid Görüntüleme ile Değerlendirilmesi Şule CEYLAN İnfertil Çiftin Fatih ÇOLAK Serdar CİNCOROP Erkek İnfertilitesinde Ampirik Tedavi Yöntemleri Mehmet Sinan ATKIN Varikosel Tanı, ve Komplikasyonları Hamit Zafer AKSOY Azospermik Olgularda Varikoselektomi Görkem AKÇA Oksidatif Stres, Sperm DNA Hasarı Tedavisi İlke Onur KAZAZ Göktuğ ATNALLAR İnmemiş Testis Fertilite Ali Ertan OKATAN Genital Travma Ürolojik Acillerde İnfertilite Olcay KARAOĞLU Çeşitli İlaçların İnfertilitesine Etkisi Recep ERYILMAZ Muhammed Hamdi SARIHAN Sporcuların Kullandığı Supplementler Murat DEMİR Radyasyon Veli DUMAN Yardımcı Üreme Teknikleri Elde Etme Serkan ÖZCAN Kök Hücre Kasım ERTAŞ KOTAN Erkeklerde Kontrasepsiyon Mücahit KABAR Klinefelter Sendromu İLERİ Tiroid Hastalıkları Üriner Enfeksiyonları Mubariz AYDAMIROV Kadir KARKIN

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Molecular characterization of DNAH6 and ATPase6 (Mitochondrial DNA) genes in asthenozoospermia patients in the northern region of India

BMC Urology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Aug. 27, 2024

Male infertility due to spermatogenesis defects affects millions of men worldwide. However, the genetic etiology vast majority remains unclear. The present study was undertaken assess association DNAH6 and ATPase6 genes in asthenozoospermia patients northern region India. A total 60 semen samples were collected for study, which 30 from case group control group. (asthenozoospermia) groups IVF Reproductive Biology Centre, Maulana Azad Medical College, New Delhi. Sperm count motility classified as per World Health Organization (WHO 2021) protocol. genomic DNA extracted stranded TRIZOL method with little modification. In-vitro molecular characterizations both checked by Polymerase Chain Reaction (PCR). 675 bp 375 amplicons amplified using PCR genes. Our results showed a significant (P ≤ 0.05) null deletion compared control. We found 45.0%, 11.7%. APTase6, it 26.7% 10.0%, respectively. concluded that presence DHAH6 had impact on male infertility.

Language: Английский

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Genetic Polymorphisms in Genes Associated with Mammalian Semen Quality Traits: A Review

Agriculture, Journal Year: 2024, Volume and Issue: 14(12), P. 2137 - 2137

Published: Nov. 25, 2024

Semen quality traits, including sperm morphology, shape, count, ejaculate volume, low percentage of motile spermatozoa in semen, and motility, play a crucial role male reproductive efficiency fertilization success. This review article highlighted the impact genetic polymorphisms genes on semen fertility traits mammalians. Single nucleotide (SNPs) these were associated with various abnormalities, such as abnormal flagella reduced impaired DNA integrity, altered antioxidant status, disrupted head–tail junction, spermatogenesis defects, testicular size, range disorders. comprehensive available literature offers significant insights into factors influencing which can contribute to development markers enhancement through assisted selective breeding programs.

Language: Английский

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Post-thaw sperm morphokinetics and mitochondrial genes profile of buck semen supplemented with selenium in two different forms

Egyptian Pharmaceutical Journal, Journal Year: 2024, Volume and Issue: 23(2), P. 382 - 390

Published: Feb. 24, 2024

Background The quality characteristics of semen is a determinant factor for improving fertility rabbit. Objectives current investigation was done to evaluate post-thaw sperm morphokinetics and mitochondrial genes expression profile buck supplemented with selenium in two different forms. Materials methods Sexually mature bucks ( n =16) that aged three months on average were used collecting ejaculates which evaluated using computer assisted analysis (CASA) program. samples all experimental animals recorded progressive motility greater than or equal 70% pooled cryopreservation. divided into groups nanoparticles normal form addition the control group. Morphological as well CASA parameters assessed after freezing 1 week. Moreover, enzymatic activity assays performed measure antioxidant capacity cryopreserved semen. Transcriptional defense regulating conducted quantitative real-time polymerase chain reaction (PCR). Results conclusion Nano forms has significantly P ≤0.05) enhanced some such DCL (µm), DAP DSL VCL (µm/s), VAP Amplitude lateral head (ALH) (µ), BCF (Hz), VSL (µm/s) during prefreezing period compared data presented present study indicated significant improvement total level (TAC) percentage live higher candidate (ATP Synthase F1 Subunit Alpha ATP5A1) , NADH dehydrogenase subunit1 ND1) subunit2 ND2), Carnitine palmitoyltransferase CPT2) ) increased In conclusion, this demonstrated enhancement pre-freezing post thaw nano selenium. proportion viability coupled up-regulation transcripts. It seems both shared same mechanism physical molecular qualities rabbit sperm.

Language: Английский

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In-silico molecular characterization of DNAH6 and ATPase6 (Mitochondrial DNA) genes in asthenozoospermia patients in the northern region of India

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: May 19, 2023

Background Male infertility due to spermatogenesis defects affects millions of men worldwide. However, the genetic etiology vast majority remains unclear. The present study was undertaken assess association DNAH6 and ATPase6 genes in asthenozoospermia patients northern region India.Methods A total 60 semen samples were collected for study, which 30 from case group control group. (asthenozoospermia) groups IVF Reproductive Biology Centre, Maulana Azad Medical College, New Delhi. Sperm count motility classified as per World Health Organization (WHO 2010) protocol. genomic DNA extracted stranded TRIZOL method with little modification.Results In-vitro molecular characterizations both checked by Polymerase Chain Reaction (PCR). 675bp 375bp amplicons amplified using PCR genes. Our results showed a significant (P ≤ 0.05) null deletion compared control. We found 45.0%, 11.7%. APTase6, it 26.7% 10.0%, respectively.Conclusions concluded that presence DHAH6 had impact on male infertility.

Language: Английский

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