The positive role of transforming growth factor-β1 in ischemic stroke

Cellular Signalling, Journal Year: 2024, Volume and Issue: 121, P. 111301 - 111301

Published: July 15, 2024

Language: Английский

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The dynamics of biomarkers of autophagy and neuroinflammation in the acute period of atherothrombotic ischemic stroke

Medical Immunology (Russia), Journal Year: 2024, Volume and Issue: 26(4), P. 843 - 852

Published: July 27, 2024

Postischemic neuroinflammation is a critical pathophysiological process within the entire pattern of cerebral ischemia. It characterized by microglial and astroglial activation accompanied disturbances in innate adaptive immune response. The early damage blood-brain barrier (BBB) integrity brain autoantigens release into circulation, particular, neurospecific protein S100B. According to recent experimental data, activated autophagy associated with postischemic neuroinflammation, involved its regulation influences outcome ischemic stroke (IS) acute period. Experimental evidence provided for involvement proinflammatory cytokines chemokines production. influence on pro- anti-inflammatory balance IS has been demonstrated. Purpose study: quantitatively evaluate key biomarkers, biomarker BBB S100B, dynamics To identify relationship between inflammation 112 patients 56 healthy persons were examined. Patients underwent dynamic clinical neurological examination blood testing 1 st , 7 th 14 days from disease’s onset. level peripheral leukocytes was determined flow cytometry assessing intracellular expression proteins LC3, p62 mean fluorescence intensity Cyto-ID dye, which specifically recognizes active autophagosomes. Serum concentrations TNFα, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-18, neuropeptide S100B biomarkers Beclin-1, ELISA. A statistically significant increase studied found compared control group. maximum indicators observed 1st, – day disease. Established correlations indicate participation stages period (days 1-7).

Language: Английский

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Cross-disease transcriptomic analysis reveals DOK3 and PAPOLA as therapeutic targets for neuroinflammatory and tumorigenic processes

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 12, 2024

Objective Subarachnoid hemorrhage (SAH) and tumorigenesis share numerous biological complexities; nevertheless, the specific gene expression profiles underlying mechanisms remain poorly understood. This study aims to identify differentially expressed genes (DEGs) that could serve as biomarkers for diagnosis prognosis. Methods Gene datasets (GSE122063, GSE13353, GSE161870) were analyzed using machine learning algorithms logistic regression DEGs associated with both SAH tumorigenesis. Lasso receiver operating characteristic (ROC) curve analysis employed evaluate classification accuracy of these genes. Validation critical was performed through pan-cancer experimental studies, focusing on role DOK3 in modulating inflammation oxidative stress U251MG glioblastoma BV2 microglia cells. Results Fifteen common identified, PAPOLA highlighted crucial implicated neurodegenerative processes. Experimental validation demonstrated overexpression significantly reduced pro-inflammatory cytokine levels markers while enhancing antioxidant enzyme activity. Additionally, influenced tumorigenic processes such apoptosis, cell cycle regulation, proliferation, effectively mitigating LPS-induced cytotoxicity microglial Conclusions play roles related neurodegeneration, presenting themselves potential prognostic therapeutic targets. Notably, exhibits an antitumor agent anti-inflammatory antioxidative properties, offering benefits cancer neuroinflammatory conditions.

Language: Английский

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Multi-target mechanism of Naoshuantong capsule for treatment of Ischemic stroke based on network pharmacology and molecular docking

Medicine, Journal Year: 2023, Volume and Issue: 102(44), P. e35771 - e35771

Published: Nov. 3, 2023

Naoshuantong capsule (NST capsule) is a classic Chinese patent medicine, which can treat ischemic stroke (IS) and has good clinical efficacy. However, its pharmacological mechanism remains to be further explored in the treatment of IS.The bio-active components potential targets NST Capsules were obtained by ETCM TCMSP databases. In addition, related IS collected Genecard, OMIM, DrugBank, TTD DisGeNET NST-IS common target was Venn platform. PPI network composition - diagram Capsule constructed Cytoscape3.8.1. Finally, AutoDock used for molecular docking.265 predicted from 32 active compounds Capsule, 109 identified between IS. The top 10 key ALB, TNF, TP53, VEGFA, CASP3, MYC, etc. Enrichment analysis showed that capsules treated mainly through lipid atherosclerosis, fluid shear stress atherosclerosis signaling pathways.Through methods pharmacology docking, this study clarified play role IS, multi-target, multi-channel multi-component regulation. This provide some references subsequent research capsule.

Language: Английский

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