Anti-Obesity Effect and Signaling Mechanism of Potassium Poly-γ-Glutamate Produced by Bacillus subtilis Chungkookjang in High-Fat Diet-Induced Obese Mice DOI Open Access

Seung-Hyeon Lee,

Jiwon Choi,

Jae Young Park

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(6), P. 809 - 809

Published: March 12, 2024

The purpose of this work was to examine the effects potassium poly-γ-glutamate (PGA-K) on mice fed a high-fat diet consisting 60% total calories for 12 weeks. PGA-K administration reduced increase in body weight, epididymal fat, and liver weight caused by compared obese group. triglyceride, low-density lipoprotein cholesterol high-density levels, which are blood lipid indicators, were significantly increased group but decreased PGA-K-treated resulted significant inhibition pro-inflammatory cytokines, including tumor necrosis factor α interleukin 6. Moreover, levels leptin insulin, insulin resistance These results suggest that exhibits protective effect against obesity induced diet, underscoring its potential as candidate treatment.

Language: Английский

A High-Throughput Screening Strategy for Bacillus subtilis Producing Menaquinone-7 Based on Fluorescence-Activated Cell Sorting DOI Creative Commons
Lina Yang,

Tang Can,

Yan Cui

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 536 - 536

Published: Feb. 27, 2025

Menaquinone-7 (MK-7) is recognized for its important biological activity, and Bacillus subtilis the preferred strain fermentative production. However, limited phenotypic diversity among high-yielding strains complicates development of rapid screening methods. To address this, we utilized effect MK-7 on transmembrane potential to develop a high-throughput (HTS) strategy efficiently identifying with improved Among various membrane fluorescent dyes tested, Rhodamine 123 was selected quantifying intracellular levels due effective staining minimal impact cell growth. By optimizing pretreatment protocols conditions, established an HTS protocol that combines fluorescence-activated sorting HPLC identify increased A linear correlation observed between mean content average fluorescence intensity (R2 = 0.9646). This approach applied mutant libraries generated through atmospheric room temperature plasma mutagenesis. After three cycles mutagenesis screening, AR03-27 identified, showing 85.65% increase in yield compared original SJTU2 strain. Resequencing analysis revealed top mutants contained mutations genes related transport, suggesting their role enhancing yield.

Language: Английский

Citations

0
Insertion of IS4Bsu1 or IS1182Bsu1 into the Oligopeptide-Permease Gene Cluster Reduces γ-Polyglutamic Acid Production in Bacillus subtilis subsp. natto DOI Creative Commons

M. Noguchi,

Yoshiyuki TANIZAKI,

Kumiko Kudo

et al.

Food Microbiology, Journal Year: 2025, Volume and Issue: 131, P. 104805 - 104805

Published: April 30, 2025

Language: Английский

Citations

0
Dual-purpose Bacillus subtilis fermentation: enhanced nattokinase production via oxygen-enriched fed-batch cultivation and natto starter preparation from harvested biomass DOI

Jiawen Zheng,

Yaping Sun,

Yunyu Liao

et al.

Bioprocess and Biosystems Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
A glimpse into plant-based fermented products alternative to animal based products: Formulation, processing, health benefits DOI
Gülden Gökşen, Qazi Sugra Altaf, Salma Farooq

et al.

Food Research International, Journal Year: 2023, Volume and Issue: 173, P. 113344 - 113344

Published: Aug. 6, 2023

Language: Английский

Citations

10
Gut microbiota alteration - Cancer relationships and synbiotic roles in cancer therapies DOI Creative Commons
Adekunle Babajide Rowaiye, Gordon C. Ibeanu, Doofan Bur

et al.

The Microbe, Journal Year: 2024, Volume and Issue: 4, P. 100096 - 100096

Published: June 2, 2024

Cancer poses a significant global health challenge, with 10 million deaths and 19.3 new cases reported annually. This review aims to investigate the emerging field of synbiotics their potential enhance effectiveness cancer therapies. By conducting thorough analysis preclinical clinical studies, this clarifies various mechanisms through which improve efficacy conventional treatments, such as chemotherapy, immunotherapy, surgery, radiotherapy. A search literature on anti-cancer was performed using PubMed database Google Scholar engine find important original research publications. selection, analysis, discussion related English-language papers were conducted. unravels intricate connections between microbiota therapeutics, harnessing power promising strategy treatment outcomes elevate standard care in oncology. Further is required establish long-term safety synbiotics, right ideal prebiotic-probiotic combinations, distinct signatures different types for development future synbiotics.

Language: Английский

Citations

3
Insight into the efficacy profile of fermented soy foods against diabetes DOI
Dibyendu Das,

Sawlang Borsingh Wann,

Jatin Kalita

et al.

Food Bioscience, Journal Year: 2023, Volume and Issue: 53, P. 102665 - 102665

Published: April 18, 2023

Language: Английский

Citations

9
Fermented soybean foods (natto) ameliorate age-related cognitive decline by hippocampal TAAR1-mediated activation of the CaMKII/CREB/BDNF signaling pathway in senescence-accelerated mouse prone 8 (SAMP8) DOI
Yifeng Zheng,

Mayu Yasuda,

Mizuki Yamao

et al.

Food & Function, Journal Year: 2023, Volume and Issue: 14(22), P. 10097 - 10106

Published: Jan. 1, 2023

Natto is a traditional fermented soybean-based food that has been an integral part of Japanese cuisine. This study demonstrated the oral administering natto ameliorated cognitive decline by activating TAAR1-mediated CaMKII/CREB/BDNF signaling pathway in hippocampus SAMP8 mice.

Language: Английский

Citations

8
Nattokinase as an adjuvant therapeutic strategy for non-communicable diseases: a review of fibrinolytic, antithrombotic, anti-inflammatory, and antioxidant effects DOI
M. Granito, Lívia Alvarenga, Márcia Ribeiro

et al.

Expert Review of Cardiovascular Therapy, Journal Year: 2024, Volume and Issue: 22(10), P. 565 - 574

Published: Oct. 2, 2024

Introduction Nattokinase (NK) is the primary ingredient of natto, a traditional Asian food made from fermented soybean by Bacillus subtilis natto. Studies have shown that natto reduces risk cardiovascular disease (CVD) mortality due to its fibrinolytic and antithrombotic properties. A new field studies also demonstrates NK can mitigate molecular pathways related inflammation oxidative stress be considered an adjuvant strategy for use in many non-communicable diseases (NCDs). This paper narrative review literature. search was conducted PubMed ScienceDirect up July 2024.

Language: Английский

Citations

3
Harnessing probiotic foods: managing cancer through gut health DOI

Devika Thapa,

Vijay Kumar, Bindu Naik

et al.

Food Science and Biotechnology, Journal Year: 2024, Volume and Issue: 33(9), P. 2141 - 2160

Published: June 28, 2024

Language: Английский

Citations

2
From plate to planet: culturally responsive culinary practices for health system innovation DOI Creative Commons
Michelle H. Loy

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Oct. 17, 2024

The field of culinary medicine has gained significant attention for its potential to improve health outcomes through the integration nutrition and medical practice. However, cultural dimensions this interdisciplinary remain underexplored. Emphasizing role sociocultural practices, paper highlights how culturally appreciative practices can meet sextuple aim healthcare system innovation. By examining diverse traditions their contributions medicine, review underscores importance attuned approaches in promoting human health. food wisdom into offers a pathway more effective personalized care, stronger patient-provider relationships, diversity/equity/inclusion/belonging, sustainable systems.

Language: Английский

Citations

2