The functions and mechanisms of long non-coding RNA in colorectal cancer DOI Creative Commons
Yuning Lin, Wenzhen Zhao,

Zhenyi Lv

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: July 4, 2024

CRC poses a significant challenge in the global health domain, with high number of deaths attributed to this disease annually. If is detected only its advanced stages, difficulty treatment increases significantly. Therefore, biomarkers for early detection play crucial role improving patient outcomes and increasing survival rates. The development reliable biomarker particularly important timely diagnosis treatment. However, current methods detection, such as endoscopic examination, blood, stool tests, have certain limitations often detect cases late stages. To overcome these constraints, researchers turned their attention molecular biomarkers, which are considered promising approach detection. Non-invasive using mRNA, circulating cell-free DNA, microRNA, LncRNA, proteins can provide more diagnostic information. These be found tissue, stool, volatile organic compounds. Identifying sensitivity specificity safe, economic, easily measurable remains researchers.

Language: Английский

Crosstalk between long non-coding RNAs and p53 signaling pathway in colorectal cancer: A review study DOI

Mohammad Mahdi Khanifar,

Zahra Zafari, Mohsen Sheykhhasan

et al.

Pathology - Research and Practice, Journal Year: 2023, Volume and Issue: 249, P. 154756 - 154756

Published: Aug. 10, 2023

Language: Английский

Citations

14
Long non‑coding RNAs as diagnostic and prognostic biomarkers for colorectal cancer (Review) DOI Open Access
Yuning Lin, Wenzhen Zhao,

Ruonan Pu

et al.

Oncology Letters, Journal Year: 2024, Volume and Issue: 28(4)

Published: Aug. 8, 2024

Colorectal cancer (CRC) ranks as the 3rd most common globally and is 2nd leading cause of cancer‑related death. Owing to lack specific early symptoms limitations existing diagnostic methods, patients with CRC are diagnosed at advanced stages. To overcome these challenges, researchers have increasingly focused on molecular biomarkers, particular interest in long non‑coding RNAs (lncRNAs). These non‑protein‑coding RNAs, which exceed 200 nucleotides length, play critical roles development progression CRC. The stability detectability lncRNAs circulatory system make them promising candidate biomarkers. analysis circulating peripheral blood represents a potential option for minimally invasive tests based liquid biopsy samples. present review aimed evaluate efficacy altered expression levels markers Additionally, clinical significance prognostic this disease were summarized.

Language: Английский

Citations

3
Second generation androgen receptor antagonist, TQB3720 abrogates prostate cancer growth via AR/GPX4 axis activated ferroptosis DOI Creative Commons
Zhongqing Zhang,

Tianlei Xie,

Shun Zhang

et al.

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: Jan. 20, 2023

Purpose: Prostate cancer (PCa) poses a great threat to humans. The study aimed evaluate the potential of TQB3720 in promoting ferroptosis suppress prostate cancer, providing theoretical basis for PCa therapy. Methods: cells and nude mice models were divided into TQB3720, enzalutamide (ENZ), control groups. Sulforhodamine B assay, colony formation assessment, organoids culture system, CCK8 assay used detecting proliferation. Western blot was processed detect expression androgen receptor (AR), ferroptosis, apoptosis-related genes. Flow cytometry applied measure intracellular ROS levels. ELISA performed determine cellular oxidized glutathione (GSSG) malondialdehyde (MDA) RT-qPCR conducted mRNA genes AR signaling. BODIPYTM™ 581/591 detection lipid peroxidation interaction with other translational factor complex proteins explored using Co-immunoprecipitation (Co-IP), chromatin immunoprecipitation (ChIP) binding AR-involved downstream promoter. Luciferase reporter examine translation activity GPX4 promoter, immunohistochemistry (IHC) analyze levels c-MYC, Ki-67 TQB3720-treated tissues. Results: Here, we found inhibits growth vitro vivo. treatment induced GSSG MDA significantly, by which hints antagonist caused ferroptosis-related cell death. Moreover, molecular evidence shown regulates signaling resulting inhibition entry nucleus. Additional, also proved that abrogates between SP1 leads decrease transcription. Conclusion: promotes reducing AR/SP1 transcriptional As result, it is suggested be drug clinic treatment.

Language: Английский

Citations

8
The functions and mechanisms of long non-coding RNA in colorectal cancer DOI Creative Commons
Yuning Lin, Wenzhen Zhao,

Zhenyi Lv

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: July 4, 2024

CRC poses a significant challenge in the global health domain, with high number of deaths attributed to this disease annually. If is detected only its advanced stages, difficulty treatment increases significantly. Therefore, biomarkers for early detection play crucial role improving patient outcomes and increasing survival rates. The development reliable biomarker particularly important timely diagnosis treatment. However, current methods detection, such as endoscopic examination, blood, stool tests, have certain limitations often detect cases late stages. To overcome these constraints, researchers turned their attention molecular biomarkers, which are considered promising approach detection. Non-invasive using mRNA, circulating cell-free DNA, microRNA, LncRNA, proteins can provide more diagnostic information. These be found tissue, stool, volatile organic compounds. Identifying sensitivity specificity safe, economic, easily measurable remains researchers.

Language: Английский

Citations

2