Osteogenic Microenvironment Restoration around Dental Implants Induced by an Injectable MXene-Based Hydrogel

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(11), P. 13071 - 13088

Published: May 23, 2024

Peri-implantitis is an excessive inflammatory response induced by complex interactions between the bacteria, host immune system, and tissues around dental implant. Alveolar bone resorption caused peri-implantitis a significant cause of implant failure. However, to restore osteogenic microenvironment, multifaceted therapeutic method that simultaneously encompasses anti-inflammatory tissue regenerative strategies indispensable. In this study, temperature-sensitive hydrogel (FMXO) loaded with MXene MgO was used develop multifunctional injectable MXene-based hydrogel. This could release in irregular defect areas situ no toxicity, either vitro or vivo. Our results showed FMXO effectively inhibited NF-κB signaling pathway RAW264.7 cells upregulated expression ALP, Runx2, OCN marrow mesenchymal stromal (BMSCs). Therefore, exerted pharmacological effects on inflammation regeneration. Moreover, we found FMXO, when exposed near-infrared (NIR) light, exhibited high efficacy against Porphyromonas gingivalis, Fusobacterium nucleatum, their plaque microorganisms, which were considered initiating factors peri-implantitis. The multifunctionality also confirmed immediate peri-implant rat model vivo, suggesting great potential for therapy clinic.

Language: Английский

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Adenosine monophosphate activated protein kinase contributes to skeletal muscle health through the control of mitochondrial function

Frontiers in Pharmacology, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 20, 2022

Skeletal muscle is one of the largest organs in body and protein repository. Mitochondria are main energy-producing organelles cells play an important role skeletal health function. They participate several biological processes related to metabolism, growth, regeneration. Adenosine monophosphate-activated kinase (AMPK) a metabolic sensor regulator systemic energy balance. AMPK involved control metabolism by regulating many downstream targets. In this review, we propose that directly controls facets mitochondrial function, which turn health. This review divided into four parts. First, summarize properties signal transduction its upstream activators. Second, discuss mitochondria myogenesis, atrophy, regeneration post-injury cells. Third, elaborate effects on biogenesis, fusion, fission autophagy, how regulates Finally, activators disease status. thus represents foundation for understanding process dynamics regulated muscle. A better dynamic essential improve hence promote

Language: Английский

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Luteolin improves vasoconstriction function and survival of septic mice via AMPK/NF-κB pathway

Heliyon, Journal Year: 2023, Volume and Issue: 9(2), P. e13330 - e13330

Published: Jan. 31, 2023

Septic shock, the leading cause of death in sepsis, is related to vasoconstriction dysfunction. To investigate effects Luteolin (LTL), a flavonoid polyphenol compound, on dysfunction septic mice and underlying mechanism, cecal ligation puncture (CLP) surgery was performed wild-type C57BL/6 induce shock. Mice were intraperitoneally injected with 0.2 mg/kg LTL within 10 min after CLP or without 20 Compound C (AMPK inhibitor) (CC) 1 h before surgery, re-administrated every 12 h. The survival rate, systolic arterial pressure (SAP), diastolic (DAP), mean (MAP) explored. After sacrificed, function, inflammatory indicators, possible regulatory signaling pathways examined. Our data showed that decreased SAP, DAP, MAP, expression ADRA1A p-AMPK/AMPK, as well increased mRNA cytokines iNOS, serum levels cytokines, p-p65/p65, p-IκBα/IκBα aortas (P < 0.05), which could be reversed by treatment 0.05). However, inhibition AMPK abolish protective In conclusion, our study manifested prevent increase via activating AMPK, suggested novel therapeutic option for patients sepsis.

Language: Английский

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ETC-1002 Attenuates Porphyromonas gingivalis Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells via the AMPK/NF-κB Pathway and Exerts Ameliorative Effects in Experimental Periodontitis in Mice

Disease Markers, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 13

Published: March 16, 2022

Background. Clinically, the failure of periodontal therapy stems largely from an inability to control inflammatory response. Resolution inflammation is active, energy-requiring repair process, not merely a passive termination inflammation. AMP-activated protein kinase (AMPK), key energy sensor, has been shown negatively regulate signaling pathways. Thus, there crucial need for new therapeutic strategies modulate AMPK and promote enhanced resolution This study aimed at investigating anti-inflammatory effects ETC-1002 through modulating in periodontitis. Methods. RAW264.7 cells were infected with Pg-LPS presence or absence ETC-1002, following which expression levels proinflammatory cytokines signaling-related proteins evaluated by real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR) western blotting. was applied murine model periodontitis determine its effect vivo. Histological changes investigated hematoxylin eosin (H&E) staining, detected using immunohistochemistry, alveolar bone height measured micro-CT imaging. Results. inhibited production cytokines, promoted phosphorylation, decreased IκBα NF-κB p65 phosphorylation Pg-LPS-treated macrophages. The inhibitory on mediators significantly abrogated siRNA-mediated silencing AMPKα cells. In vivo, cell infiltration, inflammation-mediated destruction mice experimental periodontium could be reversed administration Compound C, inhibitor. Conclusions. exerts via AMPK/NF-κB pathway vitro inhibits progress signaling-dependent manner These results provide evidence beneficial treatment

Language: Английский

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Salivary irisin level is higher and related with interleukin-6 in generalized periodontitis

Clinical Oral Investigations, Journal Year: 2023, Volume and Issue: 27(6), P. 3001 - 3008

Published: Feb. 10, 2023

Language: Английский

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IRF4 Suppresses Osteogenic Differentiation of Periodontal Ligament Stem Cells by Activating IL‐18 Signaling Pathway in Periodontitis

Advanced Biology, Journal Year: 2024, Volume and Issue: 8(11)

Published: July 30, 2024

Abstract The present study aims to investigate the role of interferon regulatory factor 4 (IRF4) in osteogenic differentiation periodontal ligament stem cells (PDLSCs) and analyze underlying signaling these processes. In this study, IRF4 is upregulated periodontitis tissues, as compared healthy tissues. knockdown increases cell proliferation, decreases levels tumor necrosis factor‐alpha, interleukin‐6, interleukin‐8, enhances activity, expression RUNX family transcription 2, Collagen I, Osteocalcin PDLSCs. opposite results are observed overexpressed Additionally, GSEA shows that activates interleukin‐18 (IL‐18) pathway. expressions IL‐18, B‐cell translocation gene interleukin‐1beta, caspase‐3 decreased by knockdown, while increased overexpression. IL‐18 overexpression eliminates promoting effect on conclusion, suppresses PDLSCs activating

Language: Английский

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Lacticaseibacillus rhamnosus MG4706 Suppresses Periodontitis in Osteoclasts, Inflammation-Inducing Cells, and Ligature-Induced Rats

Nutrients, Journal Year: 2022, Volume and Issue: 14(22), P. 4869 - 4869

Published: Nov. 17, 2022

Periodontitis is a chronic inflammatory disease characterized by tooth loss due to inflammation and the of alveolar bone. closely related various systemic diseases emerging as global health problem. In this study, we investigated anti-inflammatory effect lactic acid bacteria (LAB) in vitro on Porphyromonas gingivalis (P. gingivalis) LPS-activated RAW264.7 human gingival fibroblasts-1 (HGF-1) cells anti-osteoclastogenic LAB RANKL-induced cells. All strains (Lacticaseibacillus rhamnosus MG4706, MG4709, MG4711) inhibited nitric oxide (NO)/inducible synthase (iNOS) P. pro-inflammatory cytokines (IL-1β IL-6) matrix metalloproteinase (MMP-8 MMP-9) HGF-1 addition, treatment osteoclastogenesis reducing tartrate-resistant phosphatase (TRAP) activity cathepsin K (CtsK) through downregulation nuclear factor activated T cytoplasmic 1 (NFATc1) c-fos gene expression Administration MG4706 alleviated bone indices reduced IL-1β, IL-6, MMP-8, MMP-9, RANKL/OPG ratio tissue. conclusion, L. has potential alleviate periodontitis.

Language: Английский

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