Polydatin Ameliorates High Fructose-Induced Podocyte Oxidative Stress via Suppressing HIF-1α/NOX4 Pathway

Pharmaceutics, Journal Year: 2022, Volume and Issue: 14(10), P. 2202 - 2202

Published: Oct. 16, 2022

Long-term high fructose intake drives oxidative stress, causing glomerular podocyte injury. Polydatin, isolated from Chinese herbal medicine Polygonum cuspidatum, is used as an antioxidant agent that protects kidney function. However, it remains unclear how polydatin prevents stress-driven damage. In this study, attenuated fructose-induced expression of HIF-1α, inhibited NOX4-mediated stromal cell-derived factor-1α/C-X-C chemokine receptor type 4 (SDF-1α/CXCR4) axis activation, reduced reactive oxygen species (ROS) production in rat glomeruli and cultured podocytes. As a result, up-regulated nephrin podocin, down-regulated transient potential cation channel 6 (TRPC6) these animal cell models. Moreover, the data HIF-1α siRNA transfection showed increased NOX4 aggravated SDF-1α/CXCR4 activation HIF-1α-dependent manner, whereas to inhibit suppressed ameliorating stress These findings demonstrated fructose-driven HIF-1α/NOX4 pathway controlled Intervention disturbance by could help development therapeutic strategy combat damage associated with diet.

Language: Английский

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Polygoni Cuspidati Rhizoma et Radix extract attenuates high-fat diet-induced NAFLD in rats: Impact on untargeted serum metabolomics and liver lipidomics

Pharmacological Research - Modern Chinese Medicine, Journal Year: 2023, Volume and Issue: 9, P. 100335 - 100335

Published: Nov. 19, 2023

Polygoni Cuspidati Rhizoma et Radix (PCRR; the root and rhizome of Polygonum cuspidatum Sieb. Zucc) has long been used to treat atherosclerosis, gastrointestinal disorders, liver disease. However, underlying mechanism PCRR against steatosis remains unclear. The aim this study was determine potential therapeutic targets molecular mechanisms in improving steatosis. A high-fat diet (HFD)-induced simple model rats will be evaluate effect polyphenol-rich water extract from PCRR. Its were revealed using untargeted mass spectrometry-based serum metabolomics, hepatic lipidomic profiling approaches, qPCR, Western blotting assays. Our showed that PCRR-treated displayed significant reduction TC LDL-C, TG, levels, total lipid content under a feeding condition. Untargeted metabolomics significantly restored overall levels nine classes tissues elevated bile acids serum. We demonstrated prevents HFD-induced by regulating expressions TG metabolism proteins, including p-AMPK, p-ACC, PPARα, FAS, as well LDLR CYP7A1. Moreover, resveratrol and/or polydatin­derived metabolites solely detected group, indicating these microbial transformed might partly contribute beneficial effects can employed agent improve non-alcoholic fatty disease via metabolism.

Language: Английский

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Polydatin attenuates tubulointerstitial fibrosis in diabetic kidney disease by inhibiting YAP expression and nuclear translocation

Frontiers in Physiology, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 7, 2022

The activation of Yes-associated protein (YAP) pathway is mutually causal with the increase extracellular matrix (ECM) stiffness. Polydatin (PD) has been proved to have anti-fibrosis effect in diabetic kidney disease (DKD), but it still a mystery whether PD participates YAP-related mechano-transduction. Therefore, this study intends solve following two problems: 1) To construct an vitro system polyacrylamide hydrogels (PA gels) based on true stiffness kidneys healthy and DKD rats, observe pathological stiffness-induced YAP expression renal fibroblasts; 2) Compared verteporfin (VP), pharmacological inhibitor YAP, explore therapeutic vivo model related regulation YAP. In study, PA gels 3 kPa, 12 kPa 30 was constructed determined for first time simulate rats 8 weeks 16 weeks, respectively. stiffness, significantly increased α-smooth muscle actin (α-SMA) collagen I, production reactive oxygen species (ROS) fibroblasts, were highest. inhibited above-mentioned changes fibroblasts induced by suggesting that inhibition fibroblast-to-myofibroblast transformation ECM at least partially associated regulating mechano-transduction pathway. Importantly, inhibitory nuclear translocation similar VP, superior VP reducing urinary protein, blood glucose, urea nitrogen serum creatinine, as well decreasing α-SMA ROS overproduction fibrosis. Our results prove from biomechanical point view potential strategy delaying progression fibrosis inhibiting translocation.

Language: Английский

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Characterization, Bioaccesibility and Antioxidant Activities of Phenolic Compounds Recovered from Yellow pea (Pisum sativum) Flour and Protein Isolate

Plant Foods for Human Nutrition, Journal Year: 2024, Volume and Issue: 79(2), P. 401 - 409

Published: April 11, 2024

Language: Английский

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Reversal of neurodevelopmental impairment and cognitive enhancement by pharmacological intervention with the polyphenol polydatin in a Down syndrome model

Neuropharmacology, Journal Year: 2024, Volume and Issue: unknown, P. 110170 - 110170

Published: Sept. 1, 2024

Language: Английский

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