Chemical profiling, in-vitro and in silico α -glucosidase inhibition, antioxidant and antibacterial activities of Hypotrachyna cirrhata (Fr.) Hale ex Sipman DOI Creative Commons
Deepa Karki, Anuraj Phunyal, Tika Ram Lamichhane

et al.

All Life, Journal Year: 2024, Volume and Issue: 17(1)

Published: Nov. 14, 2024

Natural products are essential in drug development, with increasing interest lichen-derived compounds for their therapeutic potential. This study investigates the bioactivity of methanolic extract Hypotrachyna cirrhata through vitro and silico approaches. The ethyl acetate fraction demonstrated highest DPPH radical scavenging activity, an IC50 10.37 ± 0.62 μg/mL, while exhibited 72.30 0.55 μg/mL. For α-glucosidase inhibition, crude showed values 1.17 0.50 μg/mL 5.00 0.45 respectively. Antibacterial assays revealed zones inhibition 12 11 mm against Staphylococcus aureus at 25 mg/mL, strain showing sensitivity to (MIC 20.0 × 10−4 mg/mL). LC-MS analysis identified eight metabolites extract, salazinic acid (1), roccellaric (2), constictic (3), protolichesterinic (4), mannitol (5), penta hydroxyicosatrienoicacid (6), methyl pentahydroxyoxoheptacosanoate (7), one unknown compound. Five major (1-5) were selected computational highlighted that as a potential lead compound, displaying non-competitive binding affinity −9.9 kcal/mol. These findings suggest acid's promise inhibitor.

Language: Английский

Chemical profiling, in-vitro and in silico α -glucosidase inhibition, antioxidant and antibacterial activities of Hypotrachyna cirrhata (Fr.) Hale ex Sipman DOI Creative Commons
Deepa Karki, Anuraj Phunyal, Tika Ram Lamichhane

et al.

All Life, Journal Year: 2024, Volume and Issue: 17(1)

Published: Nov. 14, 2024

Natural products are essential in drug development, with increasing interest lichen-derived compounds for their therapeutic potential. This study investigates the bioactivity of methanolic extract Hypotrachyna cirrhata through vitro and silico approaches. The ethyl acetate fraction demonstrated highest DPPH radical scavenging activity, an IC50 10.37 ± 0.62 μg/mL, while exhibited 72.30 0.55 μg/mL. For α-glucosidase inhibition, crude showed values 1.17 0.50 μg/mL 5.00 0.45 respectively. Antibacterial assays revealed zones inhibition 12 11 mm against Staphylococcus aureus at 25 mg/mL, strain showing sensitivity to (MIC 20.0 × 10−4 mg/mL). LC-MS analysis identified eight metabolites extract, salazinic acid (1), roccellaric (2), constictic (3), protolichesterinic (4), mannitol (5), penta hydroxyicosatrienoicacid (6), methyl pentahydroxyoxoheptacosanoate (7), one unknown compound. Five major (1-5) were selected computational highlighted that as a potential lead compound, displaying non-competitive binding affinity −9.9 kcal/mol. These findings suggest acid's promise inhibitor.

Language: Английский

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