Single-cell atlas of endothelial cells in atherosclerosis: identifying C1 CXCL12+ ECs as key proliferative drivers for immunological precision therapeutics in atherosclerosis

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 12, 2025

Background Atherosclerosis (AS) is a chronic inflammatory disease characterized by endothelial dysfunction, monocyte infiltration, smooth muscle proliferation, and extracellular matrix accumulation. Endothelial cell (EC) dysfunction plays pivotal role in the initiation progression of AS. Despite progress traditional research methods, complexity cellular heterogeneity within remains poorly understood, necessitating more refined approach for uncovering mechanisms. Methods In this study, we employed single-cell RNA sequencing (scRNA-seq) to map landscape AS comprehensively. By analyzing heterogeneity, differentiation trajectories, functional states, identified critical subpopulations their roles Functional enrichment analyses were conducted, findings validated through vitro experiments. Results The analysis revealed distinct EC with unique contributions progression. Among these, C1 CXCL12 + ECs emerged as key subpopulation associated differentiation, vascular remodeling, inflammation. These cells demonstrated high proliferative potential enriched pathways related migration repair. Through CCK-8, Transwell assay, EdU staining angiogenesis ability, found that knockdown FOXM1 CXCL12+ resulted decreased invasion. Thus, it affects Conclusion This study provides detailed atlas AS, identifying subpopulations, regulatory pathways, factors driving application technologies paves way advancing our understanding cardiovascular diseases offers significant developing personalized therapeutic strategies immunology precision medicine.

Language: Английский

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Cross‐modal integration of bulk RNA‐seq and single‐cell RNA sequencing data to reveal T‐cell exhaustion in colorectal cancer

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(18)

Published: Sept. 1, 2024

Abstract Colorectal cancer (CRC) is a relatively common malignancy clinically and the second leading cause of cancer‐related deaths. Recent studies have identified T‐cell exhaustion as playing crucial role in pathogenesis CRC. A long‐standing challenge clinical management CRC to understand how T cells function during its progression metastasis, whether potential therapeutic targets for treatment can be predicted through cells. Here, we propose DeepTEX, multi‐omics deep learning approach that integrates cross‐model data investigate heterogeneity DeepTEX uses domain adaptation model align distributions from two different modalities applies cross‐modal knowledge distillation predict across diverse patients, identifying key functional pathways genes. offers valuable insights into application multi‐omics, providing exploring stages associated with relevant targets.

Language: Английский

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Unveiling the NEFH+ malignant cell subtype: Insights from single-cell RNA sequencing in prostate cancer progression and tumor microenvironment interactions

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 20, 2024

Prostate cancer (PCa) is a multifactorial and heterogeneous disease, ranking among the most prevalent malignancies in men. In 2020, there were 1,414,259 new cases of PCa worldwide, accounting for 7.3% all malignant tumors. The incidence rate ranks third, following breast lung cancer. Patients diagnosed with high-grade frequently present existing or developing metastases, complicating their treatment resulting poorer prognoses, particularly those bone metastases. Utilizing single-cell RNA sequencing (scRNA-seq), we identified specific cell subtypes that are closely linked to PCa. By investigating mechanisms govern interactions within tumor microenvironment (TME), aim offer theoretical insights can enhance prevention, diagnosis, PCa, ultimately striving improve patient outcomes quality life.

Language: Английский

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Cardiac Fibroblasts: Answering the Call

AJP Heart and Circulatory Physiology, Journal Year: 2024, Volume and Issue: 327(3), P. H681 - H686

Published: Aug. 2, 2024

Cardiac fibroblasts play a pivotal role in maintaining heart homeostasis by depositing extracellular matrix (ECM) to provide structural support for the myocardium, vasculature, and neuronal network contributing essential physiological processes. In response injury such as myocardial infarction or pressure overload, become activated, leading increased ECM production that can ultimately drive left ventricular remodeling progress failure. Recently,

Language: Английский

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IGFBP7+ subpopulation and IGFBP7 risk score in astrocytoma: insights from scRNA-Seq and bulk RNA-Seq

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 30, 2024

Background Glioma is the predominant malignant brain tumor that lacks effective treatment options due to its shielding by blood-brain barrier (BBB). Astrocytes play a role in development of glioma, yet diverse cellular composition astrocytoma has not been thoroughly researched. Methods We examined internal diversity seven distinct subgroups through single-cell RNA sequencing (scRNA-seq), pinpointed crucial using CytoTRACE, monocle2 pseudotime analysis, and slingshot employed various techniques identify critical subgroups, delved into communication analysis. Then, we combined clinical information GBM patients used bulk (bulk RNA-seq) analyze prognostic impact relevant molecules on patients, performed vitro experiments for validation. Results The analysis current study revealed C0 IGFBP7+ cells were noteworthy subpopulation astrocytoma, influencing differentiation progression astrocytoma. A predictive model was developed categorize high- low-scoring groups based IGFBP7 Risk Score (IGRS), with survival revealing poorer prognosis high-IGRS group. Analysis immune cell infiltration, identification genes differential expression, enrichment analyses, assessment copy number variations, evaluation drug susceptibility conducted, all which highlighted their significant influence Conclusion This research enhances comprehension delves factors impacting offers fresh perspectives treating glioma.

Language: Английский

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