Inhibition of quorum sensing regulation and stress tolerance by Rhodomyrtus tomentosa extracts and rhodomyrtone as an alternative treatment for zoonotic pathogens DOI Creative Commons

Sukanlaya Leejae,

Watcharapong Mitsuwan, Ozioma Forstinus Nwabor

et al.

Veterinary World, Journal Year: 2025, Volume and Issue: unknown, P. 877 - 887

Published: April 1, 2025

Background and Aim: Staphylococcus aureus is a zoonotic pathogen with significant public health economic implications. Its ability to tolerate environmental stress regulate virulence through quorum sensing contributes its persistence pathogenicity. Rhodomyrtus tomentosa bioactive compound rhodomyrtone have demonstrated antimicrobial properties against Gram-positive, multidrug-resistant bacteria. This study aimed evaluate the effects of these agents on inhibition tolerance in S. Pseudomonas aeruginosa, providing insights into their potential as alternative strategies. Materials Methods: The anti-quorum activity R. extracts was assessed using Chromobacterium violaceum bioindicator. In addition, P. aeruginosa swarming motility were evaluated. Stress examined by subjecting treated cells acidic (pH = 5.0), alkaline 9.0), osmotic (7.5% NaCl), heat (43°C), oxidative (1 mM H₂O₂) conditions. survival rates determined colony-forming unit (CFU) counts following treatment ethanol leaf extracts. Results: ethyl acetate fraction extract exhibited highest violacein inhibition, followed extract. At 256 μg/mL, permitted colony formation but inhibited motility. Regarding tolerance, no surviving detected under any condition after 3–6 h 2 × minimum inhibitory concentration (MIC) μg/mL) rhodomyrtone. 4 MIC (128 all tested conditions except for stresses. Conclusion: findings suggest that effectively inhibit offering promising approach. These compounds could be utilized veterinary medicine food safety mitigate contamination combat antibiotic-resistant infections. Keywords: sensing, rhodomyrtone, tomentosa, aureus, tolerance.

Language: Английский

Inhibition of quorum sensing regulation and stress tolerance by Rhodomyrtus tomentosa extracts and rhodomyrtone as an alternative treatment for zoonotic pathogens DOI Creative Commons

Sukanlaya Leejae,

Watcharapong Mitsuwan, Ozioma Forstinus Nwabor

et al.

Veterinary World, Journal Year: 2025, Volume and Issue: unknown, P. 877 - 887

Published: April 1, 2025

Background and Aim: Staphylococcus aureus is a zoonotic pathogen with significant public health economic implications. Its ability to tolerate environmental stress regulate virulence through quorum sensing contributes its persistence pathogenicity. Rhodomyrtus tomentosa bioactive compound rhodomyrtone have demonstrated antimicrobial properties against Gram-positive, multidrug-resistant bacteria. This study aimed evaluate the effects of these agents on inhibition tolerance in S. Pseudomonas aeruginosa, providing insights into their potential as alternative strategies. Materials Methods: The anti-quorum activity R. extracts was assessed using Chromobacterium violaceum bioindicator. In addition, P. aeruginosa swarming motility were evaluated. Stress examined by subjecting treated cells acidic (pH = 5.0), alkaline 9.0), osmotic (7.5% NaCl), heat (43°C), oxidative (1 mM H₂O₂) conditions. survival rates determined colony-forming unit (CFU) counts following treatment ethanol leaf extracts. Results: ethyl acetate fraction extract exhibited highest violacein inhibition, followed extract. At 256 μg/mL, permitted colony formation but inhibited motility. Regarding tolerance, no surviving detected under any condition after 3–6 h 2 × minimum inhibitory concentration (MIC) μg/mL) rhodomyrtone. 4 MIC (128 all tested conditions except for stresses. Conclusion: findings suggest that effectively inhibit offering promising approach. These compounds could be utilized veterinary medicine food safety mitigate contamination combat antibiotic-resistant infections. Keywords: sensing, rhodomyrtone, tomentosa, aureus, tolerance.

Language: Английский

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