Application of Biofire Filmarray Joint Infection Panel for Rapid Identification of Aetiology in a Necrotizing Fasciitis Case DOI Creative Commons
Zoltán Tóth, Bence Balázs, Walter P. Pfliegler

et al.

Diagnostics, Journal Year: 2024, Volume and Issue: 15(1), P. 58 - 58

Published: Dec. 29, 2024

Background: Monomicrobial Enterobacterales necrotizing fasciitis is associated with exceedingly high mortality rates. Although effective antimicrobial therapy an important part of treatment, the traditional microbiological diagnostic methods are not fast enough to meaningfully influence early therapeutic decisions. Methods: Here, we report application BioMérieux Biofire Filmarray Joint Infection Panel (BFJIP) for rapid detection causative agent and susceptibility prediction in such a case. Aside from BFJIP-based approach culturing, whole genome sequencing (WGS) was performed using Illumina MiSeq Oxford Nanopore MinION platforms. Results: The BFJIP indicated presence K. pneumoniae, without KPC, VIM, IMP, NDM, OXA-48 carbapenemase genes, CTX-M-type extended-spectrum beta-lactamases. Based on WGS data, isolate belonged K1-capsule-type ST23, harboured pNTUH-2044-like plasmid, positive all virulence factors this lineage. conventional results were also accordance results; lacked any these acquired resistance mechanisms. Conclusions: Despite being first case successful identification pathogenic bacteria necrotising assay, may become useful tool pathogens cases guiding better clinical outcomes.

Language: Английский

Metagenomics as a Transformative Tool for Antibiotic Resistance Surveillance: Highlighting the Impact of Mobile Genetic Elements with a Focus on the Complex Role of Phages DOI Creative Commons
Nikoline S. Olsen, Leise Riber

Antibiotics, Journal Year: 2025, Volume and Issue: 14(3), P. 296 - 296

Published: March 12, 2025

Extensive use of antibiotics in human healthcare as well agricultural and environmental settings has led to the emergence spread antibiotic-resistant bacteria, rendering many infections increasingly difficult treat. Coupled with limited development new antibiotics, rise antimicrobial resistance (AMR) caused a major health crisis worldwide, which calls for immediate action. Strengthening AMR surveillance systems is, therefore, crucial global national efforts combating this escalating threat. This review explores potential metagenomics, sequenced-based approach analyze entire microbial communities without need cultivation, transformative rapid tool improving strategies compared traditional cultivation-based methods. We emphasize importance monitoring mobile genetic elements (MGEs), such integrons, transposons, plasmids, bacteriophages (phages), relation their critical role facilitating dissemination determinants via horizontal gene transfer (HGT) across diverse environments clinical settings. In context, strengths limitations current bioinformatic tools designed detect AMR-associated MGEs metagenomic datasets, including emerging predictive machine learning models, are evaluated. Moreover, controversial phages transmission is discussed alongside phage therapy promising alternative conventional antibiotic treatment.

Language: Английский

Citations

0
Whole genome sequencing and evolutionary significance of a novel mosquitocidal bacterium, Bacillus thuringiensis serovar israelensis VCRC-B650 reported from Union Territory of Puducherry, India highly useful for mosquito control DOI

Kakhuangailiu Gangmei,

Mathivanan Ashokkumar,

Bhavna Gupta

et al.

Molecular Genetics and Genomics, Journal Year: 2025, Volume and Issue: 300(1)

Published: March 26, 2025

Language: Английский

Citations

0
Carbapenem-resistant Klebsiella oxytoca transmission linked to preoperative shaving in emergency neurosurgery, tracked by rapid detection via chromogenic medium and whole genome sequencing DOI Creative Commons
Yunlan Jiang, Y B Lyu, Lili Liu

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Oct. 17, 2024

This study describes the detection and tracking of emergency neurosurgical cross-transmission infections with carbapenem-resistant

Language: Английский

Citations

1
Whole Genome Sequencing of Brucella melitensis Isolates from Kuwait for the Identification of Biovars, Variants and Relationship within a Biovar DOI Creative Commons
Abu Salim Mustafa, Mohd Wasif Khan, Nazima Habibi

et al.

Medical Principles and Practice, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 15

Published: Nov. 29, 2024

Objective: The identification of Brucella genotypes is essential for epidemiological studies. whole genome sequencing emerging as a novel tool genetic characterization infectious microbes. aim this study was to genotype B. melitensis isolates from Kuwait using and variant analysis the sequence data. Methods: DNA were purified 15 heat-inactivated used prepare libraries employing Nextera XT Sample Preparation Kit (Illumina San Diego, CA, USA) sequenced on MiSeq (Illumina). files aligned three biovars melitensis, i.e., biovar 1 str. 16M, 2 63/9, 3 Ether. alignment calling performed ‘bwa mem’ SAMtools/VCFtools, respectively. Results: size all around 3.3 mega base pairs resembled 2. Single nucleotide polymorphisms (SNPs), insertions deletions, (indels) spread over genome; but 138 SNPs common among 14 isolates, supporting same ancestral origin. A neighbor-joining tree identified isolate an outlier. In addition, (2 - 478) specific each also identified, which divided into two major groups/genotypes. further showed that Kuwaiti present shared phylogeny mainly with strains Middle Eastern countries. Conclusions: Among studied in Kuwait, most prevalent melitensis. Furthermore, isolate-specific variations may be useful investigations.

Language: Английский

Citations

0
Application of Biofire Filmarray Joint Infection Panel for Rapid Identification of Aetiology in a Necrotizing Fasciitis Case DOI Creative Commons
Zoltán Tóth, Bence Balázs, Walter P. Pfliegler

et al.

Diagnostics, Journal Year: 2024, Volume and Issue: 15(1), P. 58 - 58

Published: Dec. 29, 2024

Background: Monomicrobial Enterobacterales necrotizing fasciitis is associated with exceedingly high mortality rates. Although effective antimicrobial therapy an important part of treatment, the traditional microbiological diagnostic methods are not fast enough to meaningfully influence early therapeutic decisions. Methods: Here, we report application BioMérieux Biofire Filmarray Joint Infection Panel (BFJIP) for rapid detection causative agent and susceptibility prediction in such a case. Aside from BFJIP-based approach culturing, whole genome sequencing (WGS) was performed using Illumina MiSeq Oxford Nanopore MinION platforms. Results: The BFJIP indicated presence K. pneumoniae, without KPC, VIM, IMP, NDM, OXA-48 carbapenemase genes, CTX-M-type extended-spectrum beta-lactamases. Based on WGS data, isolate belonged K1-capsule-type ST23, harboured pNTUH-2044-like plasmid, positive all virulence factors this lineage. conventional results were also accordance results; lacked any these acquired resistance mechanisms. Conclusions: Despite being first case successful identification pathogenic bacteria necrotising assay, may become useful tool pathogens cases guiding better clinical outcomes.

Language: Английский

Citations

0