Exosomes and miRNAs in Cardiovascular Diseases and Transcatheter Pulmonary Valve Replacement: Advancements, Gaps and Perspectives DOI Open Access

Rixin Liang,

Naijimuding Abudurexiti,

Jiandi Wu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13686 - 13686

Published: Dec. 21, 2024

As an important carrier of intercellular information transmission, exosomes regulate the physiological and pathological state local or distant cells by carrying a variety signal molecules such as microRNAs (miRNAs). Current research indicates that miRNAs can serve biomarkers therapeutic targets for cardiovascular diseases (CVDs). This narrative review summarizes progress their in CVDs, particularly pulmonary valve (PVDs), and, first time, explores potential associations with transcatheter replacement (TPVR). Currently, play crucial role determining optimal timing TPVR intervention, they demonstrate broad application prospects post-TPVR right ventricular (RV) remodeling, treatment, prognosis monitoring. However, association between development PVDs, regurgitation, remains unclear. The molecular mechanisms PVDs RV remodeling after have not been fully elucidated, postoperative treatment following is still its infancy. Future must focus on advancing fundamental studies, validating biomarkers, enhancing clinical applications to achieve significant breakthroughs.

Language: Английский

Biomolecule-based engineered nanoparticles for Cancer Theranostics DOI

Narayanan Parthasarathy,

Ramar Thangam, Babu Rithisa

et al.

Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 530, P. 216489 - 216489

Published: Feb. 3, 2025

Language: Английский

Citations

1
The Proteomic Landscape of the Coronary Accessible Heart Cell Surfaceome DOI Creative Commons

Iasmin Inocencio,

Alin Rai, D. Donner

et al.

PROTEOMICS, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

ABSTRACT Cell surface proteins (surfaceome) represent key signalling and interaction molecules for therapeutic targeting, biomarker profiling cellular phenotyping in physiological pathological states. Here, we employed coronary artery perfusion with membrane‐impermeant biotin to label capture the surface‐accessible proteome neo‐native (intact) heart. Using quantitative proteomics, identified 701 heart cell surfaceome accessible by artery, including receptors, enzymes, adhesion junctional molecules. This comprises 216 cardiac cell‐specific proteins, 29 reported cardiomyocytes (CXADR, CACNA1C), 12 fibroblasts (ITGA8, COL3A1) 63 multiple types (ICAM1, SLC3A2, CDH2). Further, this 53 enriched tissue compared other tissues humans implicated networks involving cardiomyopathy (CDH2, DTNA, PTKP2, SNTA1, CAM, K2D/B), muscle contraction development (ENG, SGCG, MYPN), calcium ion binding (SGCA, MASP1, THBS4, FBLN2, GSN) metabolism (SDHA, NUDFS1, GYS1, ACO2, IDH2). method offers a powerful tool dissecting molecular landscape of surfaceome, its role maintaining vascular function, potential leads studying interactions systemic delivery

Language: Английский

Citations

0
Quality Control of Fetal Wharton’s Jelly Mesenchymal Stem Cells-Derived Small Extracellular Vesicles DOI Creative Commons

Illayaraja Krishnan,

Chiew Yong Ng, Li Ting Kee

et al.

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 1807 - 1820

Published: Feb. 1, 2025

Background: Quality control (QC) is an important element in ensuring drug substances' safety, efficacy, and quality. The dosing regimen for sEVs can be the form of protein concentration or number particles based on results a series quality controls applied as in-process control. Methods: Wharton's Jelly Mesenchymal Stem Cells (WJMSCs) were isolated from four independent umbilical cord samples characterized following International Society Cellular Therapy (ISCT) guidelines. Small extracellular vesicles (sEVs) separately these WJMSCs using Tangential Flow Filtration (TFF) method per Minimal Information Studies Extracellular Vesicles (MISEV2018) Each concentrated sEV preparation was standardized its purity determined by ratio to concentration. Results: All passed (MSCs) characterization QC tests. Qualitatively, EVs-positive markers (CD63 TSG101) intact bilipid membrane detected all preparations. Quantitatively, particle concentrations revealed that preparations "impure" with < 1.5 × 10 9 particles/μg protein. Albumin co-isolated Conclusion: In short, individual pooled deemed due albumin co-isolation TFF method. For therapeutic development, it essential report EV results. Keywords: control, cord, jelly mesenchymal stem cells, tangential flow filtration, small vesicles,

Language: Английский

Citations

0
Extracellular Vesicles as Biomarkers in Infectious Diseases DOI Creative Commons

C. Cruz,

H Sodawalla, Thalachallour Mohanakumar

et al.

Biology, Journal Year: 2025, Volume and Issue: 14(2), P. 182 - 182

Published: Feb. 11, 2025

Extracellular vesicles (EVs) are nanosized that secreted by all cells into the extracellular space. EVs involved in cell-to-cell communication and can be found different bodily fluids (bronchoalveolar lavage fluid, sputum, urine), tissues, circulation; composition of reflects physiological condition releasing cell. The ability to use from for minimally invasive detection monitor diseases makes them an attractive target. carry a snapshot cell's internal state, they serve as powerful biomarkers diagnosing diseases. also play role body's immune pathogen responses. Pathogens, such bacteria viruses, exploit enhance their survival spread evade system. Changes number or contents signal presence infection, offering potential avenue developing new diagnostic methods infectious Ongoing research this area aims address current challenges range diseases, including infections There is limited literature on development using existing molecular biology approaches. We aim gap reviewing recent EV-related investigations disease studies.

Language: Английский

Citations

0
Circulating extracellular vesicles as potential biomarkers and mediators of acute respiratory distress syndrome in sepsis DOI Creative Commons
Manuela Sales Lima Nascimento, Ludmila Rodrigues Pinto Ferreira, José Mauro Vieira

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 14, 2025

The early sequence of respiratory failure events after the onset sepsis is still unknown. We hypothesize that lung should signal through circulating extracellular vesicles (EVs) when it affected by a systemic inflammatory response. Blood samples were obtained from septic patients with (n = 5) and without acute distress syndrome (ARDS) 13) at 24 h intensive care unit admission 3 days later Sírio-Libanês Hospital. Pulmonary-originated was not considered. characteristics plasma-isolated EVs compatible exosomes. 48 miRNAs evaluated real-time PCR. Comparing all + ARDS to only, 9 are transported in smaller amounts: miR-766 (-35.7, p 0.002), miR-127 (-23.8, 0.001), miR-340 (-13.5, 0.006), miR-29b (-12.8, miR-744 (-7.1, 0.05), miR-618 (-4.0, 0.02), miR-598 (-3.8, 0.035), miR-1260 (-2.5, 0.035); miR-885-5p expressed higher levels (9.5; 0.028). In paired samples, set altered generally different (p < 0.05) between (miR-1183,-1267,-1290,-17,-192,-199a-3p,-25,-485-3p,-518d,-720) or only (miR-148a,-193a-5p,-199a-3p,-222,-25,-340,744). Bioinformatic analysis showed associated ARDS, those differentially potentially target messenger RNAs Glycoprotein VI/GP6 signaling pathway. Circulating EV-miRNA cargo could be potential biomarkers inflammation during requiring mechanical ventilation.

Language: Английский

Citations

0
Extracellular vesicles: new horizons in neurodegeneration DOI Creative Commons
Jun Chen, Chen Tian, Xiong Xiao

et al.

EBioMedicine, Journal Year: 2025, Volume and Issue: 113, P. 105605 - 105605

Published: March 1, 2025

Extracellular vesicles (EVs) are lipid-enclosed nanovesicles secreted by diverse cell types that orchestrate intercellular communication through cargo delivery. Their pivotal roles span from supporting the development of normal central nervous system (CNS) to contributing pathogenesis neurological diseases. Particularly noteworthy is their involvement in propagation pathogenic proteins, such as those involved neurodegenerative disorders, and nucleic acids, closely linking them disease onset progression. Moreover, EVs have emerged promising diagnostic biomarkers for disorders tools staging, owing ability traverse blood-brain barrier specific, stable, accessible properties. This review comprehensively explores realm CNS-derived found peripheral blood, encompassing detection methods, transport mechanisms, various Furthermore, we evaluate potentials limitations clinical applications highlight prospective research directions this rapidly evolving field.

Language: Английский

Citations

0
Engineering Extracellular Vesicles Derived from 3D Cultivation of BMSCs Enriched with HGF Ameliorate Sepsis‐Induced Lung Epithelial Barrier Damage DOI Creative Commons
Yong Chen, Feihong Lin, Tong Zhang

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

Sepsis is a critical condition with high mortality, often leading to acute lung injury (ALI) due uncontrolled inflammatory responses and alveolar epithelial damage. Extracellular vesicles (EVs), particularly mesenchymal stem cell-derived EVs, have shown therapeutic potential in sepsis-related organ dysfunction by transferring RNAs proteins. However, their clinical use limited low efficacy yield. To address this, 3D-cultured MSCs (3D-MSCs) are generated using MicroTissues 3D Petri Dish. These 3D-MSCs demonstrate improved protection proliferation of MLE-12 cells vitro. Mechanistic studies conducted explore the enhanced protective effects derived EVs (3D-EVs) septic-ALI model. Proteomic molecular analyses 3D-EVs revealed that they enriched hepatocyte growth factor (HGF). HGF helps maintain barrier function damaged epithelium through PI3K-AKT signaling pathway. Overall, effectively ameliorate sepsis-induced ALI enhance prognosis enriching delivering HGF, suggesting application represents promising treatment strategy for septic ALI.

Language: Английский

Citations

0
Exosomes in Precision Oncology and Beyond: From Bench to Bedside in Diagnostics and Therapeutics DOI Open Access

Emile M. Youssef,

Dannelle Palmer,

Brandon Fletcher

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(6), P. 940 - 940

Published: March 10, 2025

Exosomes have emerged as pivotal players in precision oncology, offering innovative solutions to longstanding challenges such metastasis, therapeutic resistance, and immune evasion. These nanoscale extracellular vesicles facilitate intercellular communication by transferring bioactive molecules that mirror the biological state of their parent cells, positioning them transformative tools for cancer diagnostics therapeutics. Recent advancements exosome engineering, artificial intelligence (AI)-driven analytics, isolation technologies are breaking barriers scalability, reproducibility, clinical application. Bioengineered exosomes being leveraged CRISPR-Cas9 delivery, while AI models enhancing biomarker discovery liquid biopsy accuracy. Despite these advancements, key obstacles heterogeneity populations lack standardized protocols persist. This review synthesizes pioneering research on biology, molecular translation, emphasizing dual roles both mediators tumor progression intervention. It also explores emerging areas, including microbiome–exosome interactions integration machine learning exosome-based medicine. By bridging innovation with translational strategies, this work charts a forward-looking path integrating into next-generation care, setting it apart comprehensive guide overcoming technological hurdles rapidly evolving field.

Language: Английский

Citations

0
Therapeutic Potential of Extracellular Vesicles in Oral Inflammation DOI Open Access
Yan Yan Farm,

Bing Huan Chuah,

Jia Xian Law

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3031 - 3031

Published: March 26, 2025

The therapeutic potential of extracellular vesicles (EVs) in reducing oral inflammation is thoroughly examined this review, with an emphasis on gingivitis, periodontitis, and mucositis. It explains the complex relationship between microbial dysbiosis host immune responses aetiology inflammation. Pathophysiological mechanisms periodontitis are examined, emphasising roles played by periodontal pathogens inflammatory mediators disease's chronic course systemic effects. Preclinical research providing new evidence that EVs originating from various cellular sources control cell dynamics towards a pro-healing phenotype, promote tissue regeneration, have immunomodulatory qualities. EV-based therapies appear to be promising technique benefits over traditional methods for treatment illnesses specifically altering signalling pathways. This review highlights improve patient outcomes health emphasises need additional clinical clarify efficacy underlying therapy.

Language: Английский

Citations

0
Targeting EGFR-TKI resistance in lung cancer: Role of miR-5193/miR-149-5p loaded NK-EVs and Carboplatin combination DOI
Aakash Nathani, Li Sun, Yan Li

et al.

International Journal of Pharmaceutics, Journal Year: 2025, Volume and Issue: 675, P. 125573 - 125573

Published: April 1, 2025

Language: Английский

Citations

0