The Application of scRNA‐Seq in Heart Development and Regeneration DOI

Junying Gao,

Lindong Yu,

Haoran Qi

et al.

genesis, Journal Year: 2025, Volume and Issue: 63(2)

Published: April 1, 2025

ABSTRACT Single‐cell RNA sequencing (scRNA‐seq) is a rapidly developing and useful technique for elucidating biological mechanisms characterizing individual cells. Tens of millions patients worldwide suffer from heart injuries other types disease. Neonatal mammalian hearts certain adult vertebrate species, such as zebrafish, can fully regenerate after myocardial injury. However, the unable to damaged myocardium. scRNA‐seq provides many new insights into pathological normal facilitates our understanding cellular responses cardiac injury repair at different stages, which may provide critical clues effective therapies regeneration. In this review, we summarize application in development regeneration describe how important molecular be harnessed promote

Language: Английский

Triiodothyronine induces a proinflammatory monocyte/macrophage profile and impedes cardiac regeneration DOI Creative Commons
Ziwei Chen, Dongcheng Cai, Yifan Xie

et al.

Journal of Molecular and Cellular Cardiology, Journal Year: 2024, Volume and Issue: 191, P. 7 - 11

Published: April 11, 2024

Neonatal mouse hearts can regenerate post-injury, unlike adult that form fibrotic scars. The mechanism of thyroid hormone signaling in cardiac regeneration warrants further study. We found triiodothyronine impairs cardiomyocyte proliferation and heart neonatal mice after apical resection. Single-cell RNA-Sequencing on CD45-positive leukocytes revealed a pro-inflammatory phenotype monocytes/macrophages treatment. Furthermore, we observed was inhibited by medium from triiodothyronine-treated macrophages, while itself had no direct effect the cardiomyocytes vitro. Our study unveils novel role mediating inflammatory response hinders regeneration.

Language: Английский

Citations

4
Enhanced fibrotic potential of COL1A1hiNR4A1low fibroblasts in ischemic heart revealed by transcriptional dynamics heterogeneity analysis at both bulk and single-cell levels DOI Creative Commons

Cheng Luo,

Bangxian Tan,

Luoxiang Chu

et al.

Frontiers in Cardiovascular Medicine, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 6, 2025

Fibroblasts in the fibrotic heart exhibit a heterogeneous biological behavior. The specific subsets of fibroblasts that contribute to progressive cardiac fibrosis remain unrevealed. Our aim is identify fibroblast (FB) most significantly promote and related critical genes as biomarkers for ischemic disease. single nuclei RNA sequencing (snRNA-seq) bulk datasets used this study were obtained from Gene Expression Omnibus (GEO). activity gene sets was quantified each FB cluster using AddmoleculeScore function. Differentially expressed (DEGs) cell with highest transcription dynamics identified integrated data analysis. Multiple machine learning models employed optimal panel diagnosing disease (IHD) based on intersected DEGs. effectiveness robustness gene-derived diagnostic tool validated two independent IHD cohorts.Subsequently, we signature rat post-myocardial infarction failure model. We conducted an analysis high-quality snRNA-seq 3 4 sarcoidosis samples, resulting identification 16 clusters. Cluster2 exhibited terms fibrosis-related transcriptome dynamics. characteristic expression profile subset indicated upregulation COL1A1 several pro-fibrotic factors, including CCDC102B, GUCY1A3, TEX41, NREP, TCAP, WISP, while showing downregulation NR4A1, endogenous inhibitor TGF-β pathway. Consequently, designated subgroup COL1A1hiNR4A1low FB. set enrichment (GSEA) shows pattern closer pathways associated fibrosis. Through learning, ten feature selected construct IHD. new cohort rats. possess heightened capability promoting Additionally, it offers molecular insights into mechanisms underlying regulation Furthermore, COL1A1hiNR4A1 could serve valuable tools

Language: Английский

Citations

0
ADAMTS4 Reduction Contributes to Extracellular Matrix Deposition and Impaired Myogenesis in the Skeletal Muscle of Cigarette Smoke-Exposed Mice DOI Creative Commons
Danyang Li,

Yuqiang Pei,

Long Liang

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(2), P. 474 - 474

Published: Feb. 14, 2025

Background: The extracellular matrix (ECM) plays a critical role in the proper regeneration of skeletal muscle. ECM remodeling has been reported muscle chronic obstructive pulmonary disease (COPD), while mechanisms remain poorly understood. Methods: In this study, we examined dynamic interplay between components and enzymes COPD cigarette smoke (CS) extract-treated C2C12 cells. cells were further used to evaluate disintegrin metalloproteinase with thrombospondin motif 4 (ADAMTS4) myogenesis. Results: Chronic CS exposure induced development comorbid sarcopenia C57BL/6J mice. Muscle fibrosis was observed gastrocnemius CS-exposed mice, accompanied by an upregulation protein expression but downregulation mRNA levels fibronectin versican. We found that discrepancy attributed aberrant secretion some belonging metalloproteinases ADAMTS proteases, especially ADAMTS4. reduced ADAMTS4 muscles cells, Adamts4 knockdown versican accumulation impeded myogenic process. Conclusions: Considering recent studies have indicated impaired COPD, suggested restrained production response could be involved damaged through regulating COPD. Targeting may benefit rehabilitation COPD-related sarcopenia.

Language: Английский

Citations

0
Timestamp calibration for time-series single cell RNA-seq expression data DOI

Xiran Chen,

Sha Lin, Xiaofeng Chen

et al.

Journal of Molecular Biology, Journal Year: 2025, Volume and Issue: 437(9), P. 169021 - 169021

Published: Feb. 24, 2025

Language: Английский

Citations

0
Resveratrol suppresses growth and VCAN expression in a Cancer-associated fibroblast-breast Cancer hybrid organoid DOI
Yixin Shi, Gengxi Cai,

Chuling Zhang

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 153, P. 114451 - 114451

Published: March 17, 2025

Language: Английский

Citations

0
Fibroblast-derived versican exacerbates periodontitis progression by regulating macrophage migration and inflammatory cytokine secretion DOI
Yuting Yang, Li Li, Fang Dai

et al.

Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111755 - 111755

Published: March 1, 2025

Language: Английский

Citations

0
Indole-3-lactic acid derived from tryptophan metabolism promotes trophoblast migration and invasion by activating the AhR/VCAN pathway DOI
Yingying Wei,

Mengtian Wei,

Lu Zhang

et al.

Placenta, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Foxk1 and Foxk2 promote cardiomyocyte proliferation and heart regeneration DOI Creative Commons
Dongcheng Cai,

Chungeng Liu,

Haotong Li

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 24, 2025

Promoting endogenous cardiomyocyte proliferation is a promising strategy for cardiac repair. Identifying key factors that regulate can advance the development of novel therapies heart regeneration. Here, we identify Foxk1 and Foxk2 as regulators proliferation, whose expression declines during postnatal development. Cardiomyocyte-specific knockout or impairs neonatal regeneration after myocardial infarction (MI) injury. AAV9-mediated overexpression extends proliferative window enhances repair in adult mice MI. Mechanistically, drive cell cycle progression by directly activating CCNB1 CDK1 expression, forming CCNB1/CDK1 complex facilitates G2/M transition. Moreover, promote upregulating HIF1α which glycolysis pentose phosphate pathway (PPP), further favors proliferation. These findings establish therapeutic targets Stimulating offers approach treating injuries. authors demonstrate enhancing through activation metabolic reprogramming, presenting potential ischemic disease

Language: Английский

Citations

0
Extracellular matrix in cardiac morphogenesis, fibrosis, and regeneration DOI

Ashwini Punde,

Amey Rayrikar,

Shreya Maity

et al.

Cells and Development, Journal Year: 2025, Volume and Issue: unknown, P. 204023 - 204023

Published: March 1, 2025

Language: Английский

Citations

0
Medium from human iPSC-derived primitive macrophages promotes adult cardiomyocyte proliferation and cardiac regeneration DOI Creative Commons
Yi Xiao, Hao Zhang, Xu Liu

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 27, 2025

Heart injury has been characterized by the irreversible loss of cardiomyocytes comprising contractile tissues heart and thus strategies enabling adult cardiomyocyte proliferation are highly desired for treating various diseases. Here, we test ability human induced pluripotent stem cell-derived primitive macrophages (hiPMs) their conditioned medium (hiPM-cm) to promote enhance cardiac regeneration in mice. We find that hiPMs proliferation, which is recapitulated hiPM-cm through activation multiple pro-proliferative pathways, a secreted proteome analysis identifies five proteins participating this activation. Subsequent vivo experiments show promotes Lastly, enhances improves function injured mouse hearts. Together, our study demonstrates efficacy using promoting serve as an innovative treatment disease. Reactivating key post-injury. authors from iPSC-derived induces regeneration.

Language: Английский

Citations

0