Danqi soft caspule alleviates myocardial ischemia-reperfusion injury induced cardiomyocyte apoptosis by attenuating mitochondrial fission

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 12, 2025

Background Myocardial ischemia-reperfusion (I/R) injury which leads to continuously worsening ventricular remodeling and cardiac dysfunction in the chronic stage, is a significant contributor global prevalence of heart failure. Traditional Chinese herbal formulas have been shown prevent myocardial I/R injury. Method This study aims investigate whether Danqi soft caspule (DQ), classical traditional medicine (TCM) preparation, exerted protective effects against explore potential underlying mechanisms. A rat model cell H 2 O induced oxidative stress were established assess DQ on injury, cardiomyocyte apoptosis, as well mitochondrial structure function. Result pre-treatment reduced both proportion infarct area ischemic risk decreased apoptosis rats. In cells, was found reduce lower levels. Furthermore, inhibited fission, prevented alterations membrane potential, suppressed Cytochrome C release from mitochondria, thereby preventing apoptosis. has by reducing through inhibition fission. Moreover, could restore function suppressing phosphorylation Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) dynamin-related 1 (Drp-1). Conclusion fission associated with CaMKII Drp-1.

Language: Английский

---

Single local delivery of 5′‐(N‐ethylcarboxamido)adenosine depots ameliorates myocardial infarction‐induced cardiac dysfunction via the enhancement of mitostasis

View, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Abstract Myocardial infarction (MI) stands as a prominent contributor to global mortality. Despite existing therapies, there are notable shortcomings in delivering optimal cardiac support and reversing pathological progression, particularly within early stages. Adenosine presents promising therapeutic target; however, its clinical utility is impeded by inherent limitations. In this study, an advanced strategy using adenosine agonist pioneered ameliorate MI‐induced myocardial damage. Herein, derivative 5′‐(N‐ethylcarboxamido) (NECA) employed, efficacy evaluated via single local delivery into infarcted myocardium following MI. NECA displays remarkable benefits endothelial cells cardiomyocytes under both normoxic hypoxic conditions. Likewise, localized newly developed NECA‐loaded micro‐depots demonstrates improvement function prevention of damage MI mouse model, with promotion angiogenesis suppression inflammation, oxidation, apoptosis. Mechanistically, exerts the enhancement mitostasis triggering AMP‐activated protein kinase α (AMPKα) phosphorylation Peroxisome proliferator‐activated receptor gamma coactivator 1‐alpha(PGC‐1α) activation. These findings highlight significance recovery, single‐delivered depots providing intervention for individuals critically severe phase.

Language: Английский

---

Cardiac Tissue Engineering Using Stimuli‐Responsive Biomaterials for the Targeted Therapy of Myocardial Infarction

MedComm – Biomaterials and Applications, Journal Year: 2025, Volume and Issue: 4(1)

Published: March 1, 2025

ABSTRACT Cardiac tissue engineering presents a viable strategy for the targeted therapy of myocardial infarction (MI), overcoming limitations existing therapies in cardiac repair and regeneration. This review explores potential stimuli‐responsive biomaterials that engage with environment by reacting to various environmental stimuli including pH, temperature, enzymes, ultrasound, reactive oxygen species. These materials enable precise drug delivery, modulate cellular responses, enhance Biomaterials such as hydrogels, polymers, chitosan, collagen, alginate improve accuracy effectiveness localized delivery drugs, stem cells, growth factors, thus improving precision efficacy treatments. The looks at ability these mimic complex biochemical mechanical cues healthy myocardium. challenges prospects clinical applications are discussed, highlighting their transformative while outcomes patients MI.

Language: Английский

---

A functional cardiac patch with “gas and ion” dual-effect intervention for reconstructing blood microcirculation in myocardial infarction repair

Biomaterials, Journal Year: 2025, Volume and Issue: unknown, P. 123300 - 123300

Published: March 1, 2025

Language: Английский

---

Nanoscale piezoelectric patches preserve electrical integrity of infarcted hearts

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101742 - 101742

Published: April 1, 2025

Language: Английский

---

Recent Advances in the Development and Application of Cell-Loaded Collagen Scaffolds

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4009 - 4009

Published: April 24, 2025

Tissue engineering techniques aim to improve or replace biological tissues organs by utilizing the extracellular matrix facilitate repair of damaged organs. Collagen-based scaffolds offer numerous advantages, including excellent biocompatibility, low immunogenicity, biodegradability, hemostatic properties, and mechanical strength. Collagen can reconstruct microenvironment, promote cell adhesion, migration, proliferation, differentiation, play a critical role in cell-to-cell cell-to-matrix interactions. has been extensively utilized tissue organ reconstruction. This review examines properties collagen, its composition, structure, characteristics, regulating various cellular behaviors. Additionally, preparation cell-loaded collagen is discussed, along with comprehensive overview their applications tissues, skin, nerve, bone/cartilage, heart, liver, others. Emerging strategies future perspectives for clinical are also presented. provides synthesis mechanisms underlying use as advanced biomaterials, emphasizing potential expand collagen.

Language: Английский

---

A Piezoelectric and Suture‐Free Cardiac Patch Assembled by all FDA‐approved Materials Achieves a Minimally Invasive Gene Therapy

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Abstract Balancing biosafety with multifunctionality remains a critical challenge in biopatch development, impeding clinical translation. This study introduces minimally invasive myocardial patch fabricated entirely from FDA‐approved components, integrating piezoelectric property and sustained release of miRNA agomir. Briefly, the small intestinal submucosa (SIS) serves as matrix for situ growth β‐cyclodextrin metal‐organic framework (β‐CD‐MOF). Cholesterol‐modified miR‐210 Agomir is efficiently loaded into cyclodextrin cavities through host‐guest interactions. Flexibility water‐triggered adhesion properties, bestowed by poly (sodium thioctate) (PST) coating on rim, allow dry SIS‐Patch to fold catheter‐deliverable tube implantation. In rat infarction models, Ago‐Patch achieved release, effectively downregulating target gene expression. Simultaneously, SIS converts mechanical energy vivo electrical pulses, activating related signaling pathway. Synergistic gene‐electrical therapy improved cardiac function, increasing ejection fraction 14.0% fractional shortening 10.4%, while attenuating left ventricular remodeling fibrosis. The Ago‐Patch's dual‐action mechanism offers safe, multifunctional solution repair.

Language: Английский

---

P2X₇ Receptor Facilitates Cardiomyocyte Autophagy After Myocardial Infarction via Nox4/PERK/ATF4 Signaling Pathway

Cell Biochemistry and Function, Journal Year: 2025, Volume and Issue: 43(5)

Published: April 28, 2025

ABSTRACT Myocardial infarction (MI) represents a critical cardiovascular emergency, standing as leading cause of global mortality. ATP, typical damage‐associated molecular pattern, is stored in cells at high concentrations. Upon cellular injury, hypoxia, or necrosis, substantial quantities ATP efflux into the extracellular space, activating P2X 7 receptors, thereby initiating multiple signaling cascades. In vivo studies demonstrated coordinated upregulation and autophagy‐related proteins infarcted border zone. Transcriptome sequencing revealed Nox4 overexpression myocardial tissue post‐infarction; furthermore, administration receptor antagonist A740003 effectively reduced both protein levels expression. vitro experiments indicated that hypoxia induced Nox4, p‐PERK/PERK, ATF4, Beclin‐1, ATG5 cardiomyocytes, could inhibit expression these proteins, while counteracted this effect. Collectively, our findings was elevated zone following MI implicated its role excessive autophagy by cardiomyocytes—at least partially through Nox4/PERK/ATF4 pathway, exacerbating injury MI.

Language: Английский

---

Designing multicellular cardiac tissue engineering technologies for clinical translation

Seminars in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 171, P. 103612 - 103612

Published: April 29, 2025

Cardiovascular diseases remain the leading cause of death worldwide-claiming one-third all deaths every year. Current two-dimensional in vitro cell culture systems and animal models cannot completely recapitulate clinical complexity these humans. Therefore, there is a dire need for higher fidelity biological capable replicating phenotypes to inform outcomes therapeutic development. Cardiac tissue engineering (CTE) strategies have emerged fulfill this by design three-dimensional myocardial from human pluripotent stem cells. In way, CTE serve as highly controllable variety applications-including physiological pathological modeling, drug discovery preclinical testing platforms, even direct interventions clinic. Although significant progress has been made development technologies, critical challenges necessary refinements are required derive more advanced heart technologies. review, we distill three focus areas field address: I) Generating cardiac muscle types scalable manufacturing methods, II) Engineering structure, function, analyses, III) Curating system specific application. each our areas, emphasize importance designing mimicking intricate intercellular connectivity discuss fundamental considerations that subsequently arise. We conclude highlighting cutting-edge applications use technologies modeling repair damaged diseased hearts.

Language: Английский

---

A critical review on advances and challenges of bioprinted cardiac patches

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 189, P. 1 - 24

Published: Oct. 5, 2024

Language: Английский

---