Frontiers in Cellular Neuroscience,
Journal Year:
2021,
Volume and Issue:
15
Published: June 21, 2021
Stroke
is
a
neurological
disease
responsible
for
significant
morbidity
and
disability
worldwide.
However,
there
remains
dearth
of
effective
therapies.
The
failure
many
therapies
stroke
in
clinical
trials
has
promoted
the
development
human
cell-based
models,
such
as
brain
organoids.
Brain
organoids
differ
from
pluripotent
stem
cells
that
they
recapitulate
various
key
features
central
nervous
system
(CNS)
three-dimensional
(3D)
space.
Recent
studies
have
demonstrated
could
serve
new
platform
to
study
diseases.
are
several
limitations,
scarcity
glia
vasculature
organoids,
which
important
studying
stroke.
Herein,
we
summarized
application
organoid
technology
research,
modeling
transplantation
purposes.
We
also
discuss
methods
overcome
limitations
technology,
well
future
prospects
its
research.
Although
difficulties
challenges
associated
with
it
clear
this
approach
will
play
critical
role
exploration
treatment.
Neural Regeneration Research,
Journal Year:
2023,
Volume and Issue:
19(8), P. 1772 - 1780
Published: Nov. 8, 2023
JOURNAL/nrgr/04.03/01300535-202408000-00031/figure1/v/2024-02-22T141052Z/r/image-tiff
Proliferation
of
neural
stem
cells
is
crucial
for
promoting
neuronal
regeneration
and
repairing
cerebral
infarction
damage.
Transcranial
magnetic
stimulation
(TMS)
has
recently
emerged
as
a
tool
inducing
endogenous
cell
regeneration,
but
its
underlying
mechanisms
remain
unclear.
In
this
study,
we
found
that
repetitive
TMS
effectively
promotes
the
proliferation
oxygen-glucose
deprived
cells.
Additionally,
reduced
volume
in
rat
model
ischemic
stroke
caused
by
middle
artery
occlusion,
improved
cognitive
function,
promoted
penumbra.
RNA-sequencing
activated
Wnt
signaling
pathway
penumbra
rats
with
ischemia.
Furthermore,
PCR
analysis
revealed
AKT
phosphorylation,
leading
to
an
increase
mRNA
levels
cycle-related
proteins
such
Cdk2
Cdk4
.
This
effect
was
also
associated
activation
glycogen
synthase
kinase
3β/β-catenin
pathway,
which
ultimately
Subsequently,
validated
on
phosphorylation.
We
Ca
2+
influx
into
activating
P2
calcium
channel/calmodulin
thereby
phosphorylation
pathway.
These
findings
indicate
can
promote
through
influx-dependent
phosphorylated
AKT/glycogen
study
produced
pioneering
results
intrinsic
mechanism
function
recovery
after
stroke.
provide
strong
scientific
foundation
clinical
application
TMS.
Moreover,
treatment
may
not
only
be
efficient
potential
approach
support
neurogenesis
further
therapeutic
applications,
effective
platform
expansion
BMJ Open Science,
Journal Year:
2020,
Volume and Issue:
44(11)
Published: Jan. 1, 2020
Stroke
is
a
significant
cause
of
mortality
and
morbidity
for
which
there
are
limited
treatment
options.
Virtually
all
drug
interventions
that
have
been
successful
preclinically
in
experimental
stroke
failed
to
translate
an
effective
the
clinical
setting.
In
this
review,
we
examine
one
factors
likely
contributing
lack
translation,
failure
preclinical
studies
consider
fully
advanced
age
comorbidities
(eg,
hypertension
or
diabetes)
present
most
patients
with
stroke.
Age
affect
likelihood
suffering
stroke,
disease
progression
response
treatment.
Analysing
data
from
systematic
reviews
ischaemic
show
only
11.4%
included
aged
comorbid
model,
being
frequent.
The
degree
protection
(%
reduction
infarct
volume)
varied
depending
on
comorbidity
type
intervention.
We
reasons
attention
animals
research
discuss
value
testing
potential
therapy
models
representing
range
These
can
help
establish
any
limits
treatment's
efficacy
inform
design
trials
appropriate
patient
populations.
World Journal of Stem Cells,
Journal Year:
2021,
Volume and Issue:
13(12), P. 1905 - 1917
Published: Dec. 21, 2021
As
a
cellular
mode
of
therapy,
bone
marrow
mesenchymal
stem
cells
(BMSCs)
are
used
to
treat
stroke.
However,
their
mechanisms
in
stroke
treatment
have
not
been
established.
Recent
evidence
suggests
that
regulation
dysregulated
gut
flora
after
affects
outcomes.To
investigate
the
effects
BMSCs
on
microbiota
ischemic
stroke.A
total
30
Sprague-Dawley
rats
were
randomly
divided
into
three
groups,
including
sham
operation
control
group,
transient
middle
cerebral
artery
occlusion
(MCAO)
and
MCAO
with
BMSC
group.
The
modified
Neurological
Severity
Score
(mNSS),
beam
walking
test,
Morris
water
maze
test
evaluate
neurological
function
recovery
transplantation.
Nissl
staining
was
performed
elucidate
pathology
nerve
hippocampus.
Feces
from
each
group
collected
analyzed
by
16s
rDNA
sequencing.BMSC
transplantation
significantly
reduced
mNSS
(P
<
0.01).
Rats
better
than
revealed
exhibited
significant
improvement
learning
memory.
for
neuronal
damage
assessment
showed
orderly
arranged
necrosis.
Moreover,
regulated
microbial
structure
composition.
In
treated
BMSCs,
abundance
potential
short-chain
fatty
acid
producing
bacteria
Lactobacillus
increased.BMSC
is
therapeutic
option
stroke,
it
promotes
functions
regulating
dysbiosis.
Frontiers in Cellular Neuroscience,
Journal Year:
2021,
Volume and Issue:
15
Published: June 21, 2021
Stroke
is
a
neurological
disease
responsible
for
significant
morbidity
and
disability
worldwide.
However,
there
remains
dearth
of
effective
therapies.
The
failure
many
therapies
stroke
in
clinical
trials
has
promoted
the
development
human
cell-based
models,
such
as
brain
organoids.
Brain
organoids
differ
from
pluripotent
stem
cells
that
they
recapitulate
various
key
features
central
nervous
system
(CNS)
three-dimensional
(3D)
space.
Recent
studies
have
demonstrated
could
serve
new
platform
to
study
diseases.
are
several
limitations,
scarcity
glia
vasculature
organoids,
which
important
studying
stroke.
Herein,
we
summarized
application
organoid
technology
research,
modeling
transplantation
purposes.
We
also
discuss
methods
overcome
limitations
technology,
well
future
prospects
its
research.
Although
difficulties
challenges
associated
with
it
clear
this
approach
will
play
critical
role
exploration
treatment.
