Dynamic cerebral blood flow assessment based on electromagnetic coupling sensing and image feature analysis DOI Creative Commons
Zhiwei Gong, Lingxi Zeng, Bin Jiang

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Feb. 21, 2024

Dynamic assessment of cerebral blood flow (CBF) is crucial for guiding personalized management and treatment strategies, improving the prognosis stroke. However, a safe, reliable, effective method dynamic CBF evaluation currently lacking in clinical practice. In this study, we developed monitoring system utilizing electromagnetic coupling sensing (ECS). This detects variations brain conductivity dielectric constant by identifying resonant frequency (RF) an equivalent circuit containing both magnetic induction electrical coupling. We evaluated performance using self-made physical model vessel pulsation to test pulsatile CBF. Additionally, recruited 29 healthy volunteers monitor oxygen (CO), velocity (CBFV) data RF before after caffeine consumption. analyzed CBFV trends during immediate responses abnormal intracranial supply, induced changes vascular stiffness, compared them with CO data. Furthermore, explored dynamically assessing overall level leveraging image feature analysis. Experimental testing substantiates that provides detection range depth enhanced three four times conventional techniques, thereby comprehensively covering principal supply areas. And effectively captures under different intravascular pressure stimulations. volunteers, as stiffness increases decreases due intake, amplitude diminishes progressively. Upon extraction selection features, widely used machine learning algorithms exhibit commendable classifying levels. These results highlight our proposed methodology, predicated on ECS analysis, enables capture triggered alterations stiffness. Moreover, it accurate diagnosis varying physiological conditions.

Language: Английский

Mechanisms of Autophagy in Ineffective Reperfusion After Ischemic Stroke DOI Open Access

Shangying Bai,

Yuchuan Ding,

Leticia Simo

et al.

Journal of Neuroscience Research, Journal Year: 2025, Volume and Issue: 103(1)

Published: Jan. 1, 2025

ABSTRACT Despite significant advancements in achieving high recanalization rates (80%–90%) for large vessel occlusions through mechanical thrombectomy, the issue of “futile recanalization” remains a major clinical challenge. Futile occurs when over half patients fail to experience expected symptom improvement after recanalization, often resulting severe functional impairment or death. Traditionally, this phenomenon has been attributed inadequate blood flow and reperfusion injury. More recently, ongoing neuronal death reperfusion, which leads progression ischemic penumbra into core infarct, termed reperfusion.” This review explores complex role autophagy mechanisms futile following stroke, with focus on its relationship survival. We also examine regulation autophagic activity by epigenetic mechanisms. By investigating autophagy's we aim identify novel pathways precision treatment.

Language: Английский

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Advancements in the treatment of cerebral ischemia-reperfusion injury: Acupuncture combined with mesenchymal stem cells transplantation DOI Creative Commons
Huan Li, Jiaxin Zhang, Kewei Ma

et al.

Medicine, Journal Year: 2025, Volume and Issue: 104(2), P. e41075 - e41075

Published: Jan. 10, 2025

Cerebral ischemia-reperfusion injury (CIRI) constitutes a significant etiology of exacerbated cerebral tissue damage subsequent to intravenous thrombolysis and endovascular mechanical thrombectomy in patients diagnosed with acute ischemic stroke. The treatment CIRI has been extensively investigated through multitude clinical studies. Acupuncture demonstrated be effective treating CIRI. Recent 5 years studies have identified potential mechanisms acupuncture, including regulation autophagy, promotion angiogenesis, inhibition inflammation apoptosis, modulation cell activation, neuroplasticity regulation, nerve regeneration. transplantation mesenchymal stem cells (MSCs) can effectively suppress modulate immune responses, enhance the proliferation migration endogenous neural (NSCs), thereby compensating for NSCs deficiency following ischemia/reperfusion injury. combination acupuncture MSCs demonstrates superiority over individual treatments, significantly enhancing survival rate MSCs. Moreover, it facilitates secretion various cytokines promote their homing differentiation into functional neurons, providing novel approach

Language: Английский

Citations

0
Proteomic analysis of whole blood to investigate the therapeutic effects of nervonic acid on cerebral ischemia-reperfusion injury in rats DOI Creative Commons
Qingqing Li,

Fengrong Zhang,

Xianyu Li

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 28, 2025

Introduction Blood proteomics offers a powerful approach for identifying disease-specific biomarkers. However, no reliable blood markers are currently available the diagnosis stroke. Nervonic acid (NA), vital long-chain monounsaturated fatty found in mammalian nervous tissue, shows promising therapeutic potential neurological disorders. This study aimed to develop methodology whole identify early warning biomarkers and evaluate drug treatment efficacy. Methods After modeling via classic thread embolization method, samples were collected from rats. Morphological assessments of brain tissue indicated that NA significantly mitigated neuronal damage The differential protein expression profile was analyzed using Liquid Chromatography—Tandem Mass Spectrometry (LC-MS/MS) proteomics. Results ZZZGene Ontology (GO) analysis revealed that, compared ginkgo biloba extract (EGb), proteins differentially expressed under intervention predominantly involved oxidative stress response calcium-dependent adhesion processes. Key targets middle cerebral artery occlusion (MCAO) models included ENO1, STAT3, NME2, VCL, CCT3. Discussion proteomic provides comprehensive understanding profiles associated with disease states, offering valuable insights into enabling evaluation EGb Our findings underscore protective effects against ischemia-reperfusion injury highlight its as

Language: Английский

Citations

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Safety and Efficacy of Neuroprotective Agents as Adjunctive Therapies for Reperfusion in the Treatment of Acute Ischemic Stroke: A Systematic Review and Meta-analysis of Randomized Controlled Trials DOI
Zihui Zhang, Xinyan Wang, Kangda Zhang

et al.

Journal of Neurosurgical Anesthesiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

There is still no clear evidence of the efficacy application neuroprotective agents (NPAs) for acute ischemic stroke (AIS) patients receiving reperfusion therapies. This meta-analysis aimed to determine effects NPAs versus placebo on functional and safety outcomes as an adjunctive treatment intravenous thrombolysis (IVT) or endovascular therapy (EVT) in AIS patients. The primary outcome was neurological independence, evaluated by proportion whose modified Rankin Scale scores were 0 2 at 90 days after treatment. Thirteen randomized controlled trials with a total 3736 included. associated greater odds independence (odds ratio [OR]: 1.28; 95% CI: 1.12 1.46; P < 0.001; I = 0.0%) within days. However, subgroup analysis type (IVT, EVT, both) revealed that only EVT showed significant association between (EVT group, OR: 1.43; 1.05 1.94; 0.022; 0.0%; IVT 1.51; 0.93 2.46; 0.099; 39.8%; plus 1.17; 0.94 1.45; 0.157; 16.0%). could increase possibility undergoing therapies achieving onset; however, limited number studies each drug, further needed demonstrate individual agent different means reperfusion.

Language: Английский

Citations

0
Single‐Cell Transcriptomes of Immune Cells from Multiple Compartments Redefine the Ontology of Myeloid Subtypes Post‐Stroke DOI Creative Commons
Mo Yang, Yixiang Li, Kaibin Shi

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

Abstract The activation and infiltration of immune cells are hallmarks ischemic stroke. However, the precise origins molecular alterations these infiltrating post‐stroke remain poorly characterized. Here, a murine model stroke (permanent middle cerebral artery occlusion [p‐MCAO]) is utilized to profile single‐cell transcriptomes in brain their potential origins, including calvarial bone marrow (CBM), femur (FBM), peripheral blood mononuclear (PBMCs). This analysis reveals transcriptomically distinct populations myeloid brain‐resident after These include novel CD14 + neutrophil subpopulation that resembles CBM neutrophils. Moreover, sequential transcription factor regulatory networks neutrophils during progression delineated, many which unique population underlie acquisition chemotaxis granule release capacities. Two disease‐related cell subtypes also identified: disease inflammatory macrophages, likely deriving from circulating monocytes skull, transcriptionally immature disease‐associated microglia, possibly arising pre‐existing homeostatic microglia. Together, comprehensive survey responses performed, encompassing both local distant sites blood.

Language: Английский

Citations

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Edaravone dexborneol for ischemic stroke with sufficient recanalization after thrombectomy: a randomized phase II trial DOI Creative Commons
Dawei Chen, Zi‐Ai Zhao, Xinyu Shen

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 10, 2025

Language: Английский

Citations

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Complications of reperfusion therapy of acute ischemic stroke (scientific review) DOI Creative Commons
O.Ye. Dubenko,

Hanna Hrebeniuk,

Victoria Anysienkova

et al.

EMERGENCY MEDICINE, Journal Year: 2025, Volume and Issue: 21(1), P. 14 - 22

Published: March 15, 2025

Reperfusion therapy using intravenous thrombolysis and mechanical thrombectomy is the main type of specific treatment for ischemic stroke. However, restoration cerebral blood flow may paradoxically lead to reperfusion injury brain tissue. The causes complications are considered in this scientific review. search was carried out PubMed (https://pubmed.ncbi.nlm.nih.gov), ClinicalKey Elsevier (https://www.clinicalkey.com), Cochrane Library (https://www.cochranelibrary.com) Google Scholar (https://scholar.google.com) databases publications 2008–2024. Intracranial hemorrhage one most dangerous thrombolytic stroke, which associated with poor prognosis. Risk factors development complications, including hemorrhagic transformation a infarction, can be age, pre-stroke conditions, infarct volume. risk frequency depend on technique acute phase stroke various strategies like alteplase, tenecteplase, thrombectomy, etc. injuries have complex pathophysiological cellular biochemical mechanisms development, damage blood-brain barrier. Its increased permeability mediated by activation matrix metalloproteinases. A number laboratory biomarkers being investigated assess barrier transformation. Several studies prove that an increase level metalloproteinase 9 size Another encouraging marker caveolin-1, whose reduced levels intracranial functional outcome after endovascular therapy. Various neuroprotective reduce Modern approaches revascularization become very successful continue improve, perhaps concept will develop further.

Language: Английский

Citations

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Evaluation of cortical venous drainage in patients with Acute Ischemic Stroke DOI Creative Commons

Runyang Li,

Xi Li,

Jie Li

et al.

Frontiers in Neuroscience, Journal Year: 2025, Volume and Issue: 19

Published: April 1, 2025

The emergence of Mechanical thrombectomy (MT) has changed the treatment modalities for Acute Ischemic Stroke (AIS). But still 45 to 55% patients cannot achieve functional independence after sufficient recanalization through endovascular treatment, defined as "futile reperfusion." Poor collateral circulation and microcirculation are key factors affecting prognosis. In past, assessment these mainly focused on intracranial arteries their collateral, neglecting important role venous system in acute brain injury. More more studies have found that "poor drainage" is associated with poor However, there currently no unified standard "cortical drainage." This paper reviews pathophysiology relationship between prognosis, well methods indicators drainage," aiming provide a strong basis preoperative AIS selection plans.

Language: Английский

Citations

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Incomplete reperfusion and the presence of distal emboli in predicting clinical outcome after endovascular thrombectomy DOI Creative Commons
Amir Molaie,

Salvador Miralbés,

Bharath Naravetla

et al.

BMJ Surgery Interventions & Health Technologies, Journal Year: 2025, Volume and Issue: 7(1), P. e000345 - e000345

Published: April 1, 2025

To explore the relationship between final expanded treatment in cerebral infarction (eTICI) score and presence or absence of distal emboli on angiography clinical outcome after endovascular thrombectomy (EVT) for acute ischaemic stroke (AIS). Persistent are commonly noted, yet not all patients with intermediate eTICI scores demonstrate clear angiographic emboli, raising possibility that these differences may correlate distinct mechanisms 'no-reflow'. Therefore, we sought to better understand potential impact such markers cases incomplete reperfusion. We performed an exploratory retrospective analysis a prospectively collected group AIS who underwent EVT M1 occlusions using ASSIST Registry. 71 sites 11 countries participated registry. A total 650 were included. compared 90-day modified Rankin scale (mRS) based as well angiography. Clinical only revealed shift mRS, significant difference across predicting mRS 0-2. In grades 2b67 2c, there was trend towards when present than absent. However, pairwise comparisons levels non-significant. those persistent trended outcomes. With reperfusion, identifying be useful distinguishing patterns These findings should followed by investigations correlation other microcirculatory NCT03845491.

Language: Английский

Citations

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Efficacy and safety of intra-arterial thrombolysis following successful endovascular thrombectomy in patients with large vessel occlusion stroke: A meta-analysis DOI
Zhi Yang, Wentai Zhang, Yonggang Xu

et al.

European Stroke Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Introduction: The impact of intra-arterial thrombolysis (IAT) following successful endovascular thrombectomy (EVT) in patients with acute ischemic stroke (AIS) due to large vessel occlusion (LVO) remains uncertain. This study aims assess the efficacy and safety IAT as an adjunct EVT AIS-LVO. Patients methods: We searched PubMed, Embase, Cochrane databases identify randomized controlled trials (RCTs) that compared + EVT-only for primary outcome was excellent functional outcome, defined a modified Rankin Scale (mRS) score 0–1 at 90 days. Secondary outcomes included independence (mRS 0–2), symptomatic intracranial hemorrhage (sICH), mortality. pooled data were analyzed using random-effects models. Discussion conclusion: A total four RCTs, involving 1395 patients, analysis. results showed who received had significantly higher likelihood achieving days those (risk ratio [RR], 1.16; 95% confidence interval [CI], 1.03–1.31). No statistically significant differences observed between groups terms 90-day (RR, 1.03; CI, 0.94–1.13), sICH 1.30; 0.80–2.13), or mortality 0.94; 0.76–1.17). Conclusions: Among AIS-LVO have undergone EVT, use IAT, no additional treatment, associated greater Systematic review registration: CRD42024602099

Language: Английский

Citations

0