Chronic sleep deprivation impairs retinal circadian transcriptome and visual function

Experimental Eye Research, Journal Year: 2024, Volume and Issue: 243, P. 109907 - 109907

Published: April 21, 2024

Language: Английский

BMAL1 Overexpression in Suprachiasmatic Nucleus Protects from Retinal Neurovascular Deficits in Diabetes

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Abstract The suprachiasmatic nucleus (SCN) regulates circadian rhythms and influences physiological behavioral processes. Disruptions in (CRD) are observed type 2 diabetes (T2D), importantly, CRD acts as an independent risk factor for T2D its associated complications. BMAL1, a clock gene, is vital sustaining optimal rhythm function. However, the therapeutic potential of BMAL1 overexpression SCN to rectify neurovascular deficits has yet be investigated. In this study, db/db mice, well-established model exhibiting arrhythmic behavior complications diabetes, were injected stereotaxically with AAV8-Bmal1 or control virus evaluate protective effects correcting central on deficits. Given complex network eye’s unique accessibility transparent system, ocular selected examine neuronal functional, behavioral, vascular benefits clock. normalized rhythms, demonstrated by improvements free-running period. retinal function improved electroretinogram, along optomotor visual acuity enhancements. Retinal also significantly reduced. Notably, our approach helped decrease fat content genetically predisposed obese animals. Since known regulate hepatic glucose production via sympathetic mechanisms, glycemic control, pyruvate tolerance tests conducted. Systemically, we homeostasis BMAL1-overexpressing mice alongside substantial reduction gluconeogenesis. lowered plasma norepinephrine liver TH levels, indicating regulation adrenergic signaling. Thus, study underscores targeting genes like alleviate metabolic T2D. Our research offers compelling framework integrating into managing

Language: Английский

Association between rest-activity rhythm and diabetic retinopathy among US middle-age and older diabetic adults

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 16, 2024

Background The disruption of circadian rhythm has been reported to aggravate the progression diabetic retinopathy (DR). Rest-activity (RAR) is a widely used method for measuring individual time influencing behavior. In this study, we sought explore potential association between RAR and risk DR. Methods Diabetic participants aged over 40 from 2011-2014 National Health Nutrition Examination Survey (NHANES) were enrolled. Data wearable device ActiGraph GT3X was generate metrics, including interdaily stability (IS), intradaily variability (IV), most active 10-hour period (M10), least 5-hour (L5), Relative amplitude (RA). Weighted multivariable logistic regression analysis restricted cubic spline conducted examine metrics DR risk. Sensitivity also robustness findings. An unsupervised K-means clustering identify patterns in IV M10. Results A total 1,096 enrolled, with prevalence 20.53%. mean age 62.3 years, 49.57% being male. After adjusting covariates, positively associated (β: 3.527, 95%CI: 1.371-9.073). Compared lowest quintile IV, highest had 136% higher odds contrast, M10 negatively 0.902, 0.828-0.982), showing 48.8% lower Restricted confirmed that these associations linear. Meanwhile, sensitivity robustness. identified three distinct clusters, Cluster C (high-IV, low-M10) significantly comparing (low-IV, high-M10). Conclusion more fragmented peak activity level might be an increased These findings indicate maintaining rhythmic sleep-activity behavior mitigate development Further research necessary establish causality understand underlying mechanisms, focus on whether interventions designed enhance daily increase diurnal can effectively

Language: Английский