Transcriptional regulators FOXD1 and RBFOX2 contribute to metastatic capacity inBAP1mutuveal melanoma DOI Creative Commons
Quincy C. C. van den Bosch, Josephine Q.N. Nguyen,

Nikki Konemann

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Abstract Uveal melanoma is the most prevalent primary intraocular cancer, with a significant metastatic risk. This risk dependent on genetic drivers. Secondary mutations in EIF1AX, SF3B1 and BAP1 correlate clinical outcomes are recognized for their distinct transcriptomic epigenetic profiles. Previously, we identified 480 genes involved development of ocular melanocytes. Top ranking RBFOX2 FOXD1 were significantly associated UM independently correlated to poor progression. However, it uncertain whether either or have biological contribution disease progression solely indicative. study investigates if how these high-risk transcription regulators could influence tumor through knock-out overexpression models. Our results indicate that loss affects cell morphology, attachment proliferation, particularly neg cells. Additionally, both contribute growth dissemination zebrafish xenografts. Loss reduced volume dissemination, greatest effects seen Overexpression models demonstrated different morphological invasive behavior depending background, suggesting complex roles context fashion. did not alter pos cells yet made more aggressive vitro vivo . underscores as important factors

Language: Английский

Transcriptional regulators FOXD1 and RBFOX2 contribute to metastatic capacity inBAP1mutuveal melanoma DOI Creative Commons
Quincy C. C. van den Bosch, Josephine Q.N. Nguyen,

Nikki Konemann

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Abstract Uveal melanoma is the most prevalent primary intraocular cancer, with a significant metastatic risk. This risk dependent on genetic drivers. Secondary mutations in EIF1AX, SF3B1 and BAP1 correlate clinical outcomes are recognized for their distinct transcriptomic epigenetic profiles. Previously, we identified 480 genes involved development of ocular melanocytes. Top ranking RBFOX2 FOXD1 were significantly associated UM independently correlated to poor progression. However, it uncertain whether either or have biological contribution disease progression solely indicative. study investigates if how these high-risk transcription regulators could influence tumor through knock-out overexpression models. Our results indicate that loss affects cell morphology, attachment proliferation, particularly neg cells. Additionally, both contribute growth dissemination zebrafish xenografts. Loss reduced volume dissemination, greatest effects seen Overexpression models demonstrated different morphological invasive behavior depending background, suggesting complex roles context fashion. did not alter pos cells yet made more aggressive vitro vivo . underscores as important factors

Language: Английский

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