Corneal epithelial cells upregulate macropinocytosis to engulf metabolically active axonal mitochondria released by injured axons DOI Creative Commons

Sonali Pal‐Ghosh,

Himani Datta-Majumdar,

Shyamali Datta

et al.

The Ocular Surface, Journal Year: 2025, Volume and Issue: 37, P. 173 - 188

Published: April 7, 2025

To determine the mechanisms used to internalize mitochondria by corneal epithelial cells after in vivo trephine injury and vitro cells. Male female mice were subjected euthanized immediately, 6, 24 hours injury. Macropinocytosis was quantified using 70 kD fluorescent dextran. Mitochondrial content assessed immunofluorescence metabolic activity Seahorse assay immediately 6 In experiments human limbal (HCLE) isolated performed assess mitochondrial transfer presence of gap junction inhibitor 18α-glycyrrhetinc acid macropincytosis ethylisopropylamiloride. Mitochondria accumulate within apical cell layers minutes also increases Oxygen Consumption Rates increase epithelium males females. Inhibiting junctions engulfment while inhibiting macropinocytosis prevents vitro. Molecules released injured severed axons induce An oxygen consumption rate indicates that axonal can evade lysosomal degradation for at least hours. studies labeled unlabeled control mechanically stressed confirm involvement free vesicle bound

Language: Английский

Corneal epithelial cells upregulate macropinocytosis to engulf metabolically active axonal mitochondria released by injured axons DOI Creative Commons

Sonali Pal‐Ghosh,

Himani Datta-Majumdar,

Shyamali Datta

et al.

The Ocular Surface, Journal Year: 2025, Volume and Issue: 37, P. 173 - 188

Published: April 7, 2025

To determine the mechanisms used to internalize mitochondria by corneal epithelial cells after in vivo trephine injury and vitro cells. Male female mice were subjected euthanized immediately, 6, 24 hours injury. Macropinocytosis was quantified using 70 kD fluorescent dextran. Mitochondrial content assessed immunofluorescence metabolic activity Seahorse assay immediately 6 In experiments human limbal (HCLE) isolated performed assess mitochondrial transfer presence of gap junction inhibitor 18α-glycyrrhetinc acid macropincytosis ethylisopropylamiloride. Mitochondria accumulate within apical cell layers minutes also increases Oxygen Consumption Rates increase epithelium males females. Inhibiting junctions engulfment while inhibiting macropinocytosis prevents vitro. Molecules released injured severed axons induce An oxygen consumption rate indicates that axonal can evade lysosomal degradation for at least hours. studies labeled unlabeled control mechanically stressed confirm involvement free vesicle bound

Language: Английский

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