Current Eye Research, Journal Year: 2023, Volume and Issue: 49(3), P. 314 - 324
Published: Dec. 26, 2023
Purpose To compare peak cone density predicted from outer segment length measured on optical coherence tomography with direct measures of adaptive optics scanning light ophthalmoscopy.
Language: Английский
Communications Engineering, Journal Year: 2024, Volume and Issue: 3(1)
Published: Jan. 31, 2024
Abstract Visible light optical coherence tomography (VIS-OCT) is an emerging ophthalmic imaging method featuring ultrahigh depth resolution, retinal microvascular oximetry, and distinct scattering contrast in the visible spectral range. The clinical utility of VIS-OCT hampered by fundamental trade-off between range axial which are determined resolution bandwidth, respectively. To address this trade-off, here we developed a dual-channel system with three major advancements including first linear-in-K spectrometer to decrease roll-off, reference pathlength modulation expand range, per-A-line noise cancellation remove excess noise, Due these unique designs, achieves 7.2 dB roll-off over full 1.74 mm (water) shot-noise limited performance. uniquely enables >60° wide-field would allow simultaneous peripheral retina optic nerve head, as well 1.3 µm (water). Benefiting from additional near-infrared (NIR) channel design, compatible Doppler OCT angiography (OCTA). comprehensive structure-function measurement enabled advance towards adoption applications.
Language: Английский
Citations
13
Translational Vision Science & Technology, Journal Year: 2025, Volume and Issue: 14(3), P. 2 - 2
Published: March 3, 2025
To evaluate outer retinal organization in normal subjects and those using hydroxychloroquine (HCQ) with ultrahigh-resolution visible-light optical coherence tomography (VIS-OCT). Forty eyes of 22 adult were recruited from a tertiary-care retina practice, including controls (20 eyes, 12 subjects, 40 ± years old) history HCQ use 10 62 17 old). Each subject was imaged custom-built VIS-OCT device (axial resolution 1.3 µm) U.S. Food Drug Administration-approved OCT devices. With the VIS-OCT, control demonstrated five six hyperreflective bands foveal parafoveal regions, respectively, between nuclear layer Bruch's membrane. These intensity profiles complementary to known histopathologic distribution rods cones. In comparison controls, taking reduced all bands, particularly two four. cases suspected or toxicity, attenuation band 3i, no there other that more severe than suggesting changes reflectivity 3i may be earliest identifiable sign toxicity. revealed unique banding pattern corresponding photoreceptor density distributions. Notable segment profile associated early This finding an early, possibly reversible, primarily impacting is useful detecting subclinical structural found hydroxychloroquine.
Language: Английский
Citations
1
Translational Vision Science & Technology, Journal Year: 2025, Volume and Issue: 14(1), P. 16 - 16
Published: Jan. 15, 2025
To evaluate visibility of a sub-band posterior to the external limiting membrane (ELM) and assess its age-associated variation. In retrospective cross-sectional study, normal eyes were imaged using high-resolution spectral-domain optical coherence tomography (SD-OCT) prototype (2.7-µm axial resolution). Volume fusion six sequential scans (each 500 × A-scans over 6 mm mm) was performed in motion correction volume reconstruction OCT (MoReOCT) framework enhance feature OCT. The subjects divided into three groups: young (21-40 years old), middle (41-60 older (>60 old). Three expert graders assessed on B-scans, A-scan intensity relative ELM (peak ratio) measured. Forty-four 44 imaged. sub-band, tentatively attributed photoreceptor myoid, can be visualized under B-scan gradings showed that increased with age (visible 16.7%, 47.2%, 66.7% young, middle, groups, respectively). statistically different among groups at 1 2 nasal temporal (P < 0.04) from foveal center. Similarly, mean peak ratios 71.6%, 77.5%, 85.2% respectively, positively correlated = 0.012) 0.001). High-resolution OCT, combined advanced fusion, enables visualization myoid investigation variations. Investigating advance our understanding photoreceptors their association aging disease pathogenesis.
Language: Английский
Citations
0
Ophthalmology Science, Journal Year: 2023, Volume and Issue: 3(4), P. 100321 - 100321
Published: April 29, 2023
Although the outer nuclear layer (ONL) and plexiform (OPL) each exhibit a complex internal organization, near-infrared OCT depicts both as monolithic bands. Here, using visible light in C57BL/6J mouse retina, sublaminar age-related changes photoreceptor features were imaged interpreted. These (1) oscillations reflectivity, or striations, ONL (2) moderately reflective subband OPL.Cross-sectional study.Pigmented mice (C57BL/6J, n = 14).A 1.0-μm axial resolution spectral/Fourier domain system was used for vivo retinal imaging. Light electron microscopy performed ex vivo. Linear mixed effects models regression employed statistical analysis.Comparison of subbands with corresponding histological features, well quantification thickness reflectivity.Corresponding comparisons confirm that striations arise from rowlike arrangement nuclei reveal OPL arises rod spherules. Compression age suggests soma organization. Thinning supports reduction synapses OPL. Critically, somas are tightly correlated purported spherule but not rest OPL.Visible imaging resolves postsynaptic synaptic differences. Visible can study to synapse living retina.Proprietary commercial disclosure may be found after references.
Language: Английский
Citations
9
Optics Letters, Journal Year: 2023, Volume and Issue: 48(18), P. 4737 - 4737
Published: Aug. 7, 2023
We demonstrate superluminescent diodes (SLDs) for visible light optical coherence tomography (OCT) of the human retina. SLDs are less costly than supercontinuum sources and have lower intrinsic excess noise, enabling imaging closer to shot noise limit. While single not broadband, they provide power concentrated at specific wavelengths relevant retinal function. As a new, best our knowledge, application, we image macular pigments (MPs), which thought both aid vision protect against advanced age-related degeneration. Using unique depth-resolved capabilities OCT, localize MPs in depth Henle's fibers beneath foveal pit living Our approach reduces cost OCT nearly that near-infrared (NIR) while also providing information about clinically cannot be measured NIR.
Language: Английский
Citations
5
Biomedical Optics Express, Journal Year: 2023, Volume and Issue: 14(11), P. 6088 - 6088
Published: Oct. 20, 2023
Visible light optical coherence tomography (VIS-OCT) of the human retina is an emerging imaging modality that uses shorter wavelengths in visible range than conventional near-infrared (NIR) light. It provides one-micron level axial resolution to better separate stratified retinal layers, as well microvascular oximetry. However, due practical limitation laser safety and comfort, permissible illumination power much lower NIR OCT, which can be challenging obtain high-quality VIS-OCT images subsequent image analysis. Therefore, improving quality by denoising essential step overall workflow clinical applications. In this paper, we provide first dataset from normal eyes, including layer annotation “noisy-clean” pairs. We propose efficient co-learning deep learning framework for parallel self-denoising segmentation simultaneously. Both tasks synergize within same network improve each other’s performance. The significant improvement (2% higher Dice coefficient compared segmentation-only process) ganglion cell (GCL), inner plexiform (IPL) nuclear (INL) observed when available drops 25%, suggesting annotation-efficient training. also showed model trained on our generalizes a different scanning protocol.
Language: Английский
Citations
4
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown
Published: Oct. 7, 2022
Abstract Visible light optical coherence tomography (VIS-OCT) is an emerging ophthalmic imaging method uniquely featured by ultrahigh depth resolution, retinal microvascular oximetry, and distinct scattering contrast in the visible spectral range. However, clinical utility of VIS-OCT impeded fundamental trade-off between range axial determined resolution bandwidth respectively. While full potential leveraged a broad bandwidth, inversely sacrificed. The effective further limited wavelength-dependent roll-off that signal-to-noise ratio (SNR) reduces deeper range, more so shorter wavelength. To address this trade-off, we developed second-generation dual-channel system including first linear-in-k spectrometer, reference pathlength modulation, per A-line noise cancellation. All combined, have achieved 7.2dB over 1.74 mm (water) with shot-noise performance. enables >60° wide-field large curvature at peripheral retina optic nerve head, as well high-definition 1.3 um (water). design includes conventional near infrared (NIR) channel, compatible Doppler OCT angiography (OCTA). comprehensive structure-function measurement 2 nd -Gen significant advance towards broader adaptation applications.
Language: Английский
Citations
5
Eye, Journal Year: 2023, Volume and Issue: 38(5), P. 863 - 870
Published: Oct. 24, 2023
Language: Английский
Citations
1
2022 International Conference on Communication, Computing and Internet of Things (IC3IoT), Journal Year: 2024, Volume and Issue: unknown, P. 1 - 6
Published: April 17, 2024
A revolutionary imaging technique called optical coherence tomography of visible light (VIS-OCT) the individual uses shorter wavelengths than traditional near-infrared (NIR) light. To more accurately discern stratified retinal layers, it offers microvascular oximetry together with one-micron level axial resolution. Since allowed illumination power is significantly lower NIR OCT due to practical limits regarding laser safety and comfort, might be difficult generate VIS-OCT images a high enough quality for further image processing. As result, denoising crucial step in whole workflow clinical applications involving VIS-OCT. The first collection presented this study from normal eyes obtained using We offer simultaneous self-denoising segmentation system based on deep learning. Both tasks complement one another inside same network increase each other's productivity. Annotation-efficient training demonstrated by discernible When available annotation falls below 25%, Dice coefficient was 2% higher segmentation-only method.) accomplished. Additionally, we how well model learned our dataset could applied an alternative scanning method.
Language: Английский
Citations
0
Biomedical Optics Express, Journal Year: 2024, Volume and Issue: 15(8), P. 4749 - 4749
Published: July 11, 2024
Precise interpretation of the anatomical origins outer retinal optical coherence tomography (OCT) presents technical challenges owing to delicate nature retina. To address this challenge, our study introduces a novel polarization-sensitive full-field swept-source OCT (FF-SS-OCT) that provides parallel-polarization and cross-polarization measurements, predominantly capturing ballistically reflected photons multiply scattered photons, respectively. Notably, unveils layer-like structures more effectively, including inner plexiform layer (IPL) sub-layers, (OPL) sub-layers (in rod-dominant regions), rod/cone segment (OS) tips, compared OCT, where such are not visible. Through comparative analysis images retina, we discovered 2 nd band results from contributions both ellipsoid zone (EZ) segment/outer (IS/OS) junction. Similarly, 3 rd appears reflect interdigitation (IZ) photoreceptor OS tips. This approach advances understanding signals proposes potential new biomarkers for assessing health diseases.
Language: Английский
Citations
0