Role of Endometrial Extracellular Vesicles in Mediating Cell-to-Cell Communication in the Uterus: A Review

Cells, Journal Year: 2023, Volume and Issue: 12(22), P. 2584 - 2584

Published: Nov. 7, 2023

There are several critical events that occur in the uterus during early pregnancy which necessary for establishment and maintenance of pregnancy. These include blastocyst implantation, uterine decidualization, neoangiogenesis, differentiation trophoblast stem cells into different cell lineages, formation a placenta. processes involve types within pregnant uterus. Communication between these must be intricately coordinated successful embryo implantation functional maternal–fetal interface Understanding how this intricate coordination transpires has been focus researchers field many years. It long understood maternal endometrial tissue plays key role intercellular signaling pregnancy, sending signals to nearby tissues paracrine manner. Recently, insights have obtained mechanisms by occur. Notably, endometrium shown secrete extracellular vesicles (EVs) contain crucial cargo (proteins, lipids, RNA, miRNA) taken up recipient initiate response leading occurrence placentation. In review, we aim summarize endometrium-derived EVs play mediating cell-to-cell communications orchestrate establish maintain We will also discuss aberrant EV may lead pathophysiological conditions, such as endometriosis infertility.

Language: Английский

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The impact of ovarian stimulation on the human endometrial microenvironment

Human Reproduction, Journal Year: 2024, Volume and Issue: 39(5), P. 1023 - 1041

Published: March 20, 2024

Abstract STUDY QUESTION How does ovarian stimulation (OS), which is used to mature multiple oocytes for ART procedures, impact the principal cellular compartments and transcriptome of human endometrium in periovulatory mid-secretory phases? SUMMARY ANSWER During window implantation, OS alters abundance endometrial immune cells, whereas during period, substantially changes impacts both glandular cells. WHAT IS KNOWN ALREADY Pregnancies conceived an cycle are at risk complications reflective abnormal placentation placental function. can alter gene expression cell populations. glandular, stromal, immune, vascular endometrium, period as compared unknown. DESIGN, SIZE, DURATION This prospective cohort study carried out between 2020 2022 included 25 subjects undergoing natural menstrual cycles. Endometrial biopsies were performed proliferative, periovulatory, phases. PARTICIPANTS/MATERIALS, SETTING, METHODS Blood samples processed determine serum estradiol progesterone levels. Both including glands, stroma, vasculature, evaluated by examining dating, differential expression, protein populations, three-dimensional structure tissue. Mann–Whitney U tests, unpaired t-tests or one-way ANOVA pairwise comparison tests statistically evaluate differences. MAIN RESULTS AND THE ROLE OF CHANCE In induced high levels glandular-stromal dyssynchrony, increase epithelial volume frequency monocytes/macrophages. implantation phase, with a greater B cells lower CD4 effector T LARGE SCALE DATA The data underlying this article have been uploaded Genome Expression Omnibus/National Center Biotechnology Information accession number GSE220044. LIMITATIONS, REASONS FOR CAUTION A limited study, although within each group, OS, homogenous their clinical characteristics. utilized was sufficient identify significant differences; however, larger additional power, we may detect Another limitation that proliferative phase collected cycles, but not Given did known factor infertility, comparisons involved who had similar robust response stimulation, findings generalizable women normal OS. WIDER IMPLICATIONS FINDINGS altered fewer transcriptomic type-specific phase. Our show after microenvironment possesses many more similarities than endometrium. Further investigation compartment functional significance under conditions warranted. FUNDING/COMPETING INTERESTS Research reported publication supported National Institute Allergy Infectious Diseases (R01AI148695 A.M.B. N.C.D.), Eunice Kennedy Shriver Child Health Human Development (R01HD109152 R.A.), March Dimes (5-FY20-209 R.A.). content solely responsibility authors necessarily represent official views Institutes Dimes. All declare no conflict interest.

Language: Английский

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Fetus Exposure to Drugs and Chemicals: A Holistic Overview on the Assessment of Their Transport and Metabolism across the Human Placental Barrier

Diseases, Journal Year: 2024, Volume and Issue: 12(6), P. 114 - 114

Published: June 1, 2024

Background: The placenta exerts a crucial role in fetus growth and development during gestation, protecting the from maternal drugs chemical exposure. However, diverse chemicals (xenobiotics) can penetrate placental barrier, leading to deleterious, adverse effects concerning health. Moreover, enzymes metabolize into more toxic compounds for fetus. Thus, evaluating molecular mechanisms through which transfer undergo metabolism across barrier is of vital importance. In this aspect, comprehensive literature review aims provide holistic approach by critically summarizing scrutinizing potential processes governing may lead fetotoxicity effects, as well analyzing currently available experimental methodologies used assess xenobiotics metabolism. Methods: A in-depth was conducted most accurate scientific databases such PubMed, Scopus, Web Science using relevant effective keywords related xenobiotic metabolism, retrieving 8830 published articles until 5 February 2024. After applying several strict exclusion inclusion criteria, final number 148 were included. Results: During pregnancy, be transferred mother either passive diffusion or transporters, resulting exposure effects. Some also appear metabolized products both At present, there increasing research determine drug All have specific strengths limitations, highlighting strong demand utilize an efficient combination them obtain reliable evidence barrier. To derive consistent safe evidence, vitro studies, ex vivo perfusion methods, animal human studies applied together with aim minimize Conclusions: Research being increasingly carried out evaluation transport advanced techniques avoid fetotoxic improvement novel protocols are major importance protect exposure,

Language: Английский

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Mechanisms of endometrial aging: lessons from natural conceptions and assisted reproductive technology cycles

Frontiers in Physiology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 28, 2024

Until recently, the study of age-related decline in fertility has focused primarily on ovary; depletion finite pool oocytes and increases meiotic errors leading to oocyte aneuploidy are well-established mechanisms by which declines with advancing age. Comparatively little is known about impact age endometrial function. The endometrium a complex tissue comprised many cell types, including epithelial, stromal, vascular, immune stem cells. capacity this for rapid, cyclic regeneration unique tissue, undergoing repeated cycles growth shedding (in absence an embryo) response ovarian hormones. Furthermore, been shown be capable supporting pregnancies beyond established boundaries reproductive lifespan. Despite its longevity, molecular studies have changes individual populations within endometrium. Human clinical attempted isolate effect aging analyzing conceived euploid, high quality embryos. In review, we explore existing literature pregnancy outcomes. We begin overview principles physiology then behind cellular compartments. Finally, highlight lessons gleaned from rodent human propose opportunities future better understand contribution fertility.

Language: Английский

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Signaling via retinoic acid receptors mediates decidual angiogenesis in mice and human stromal cell decidualization

The FASEB Journal, Journal Year: 2025, Volume and Issue: 39(1)

Published: Jan. 7, 2025

Abstract At the maternal‐fetal interface, tightly regulated levels of retinoic acid (RA), physiologically active metabolite vitamin A, are required for embryo implantation and pregnancy success. Herein, we utilize mouse models, primary human cells, pharmacological tools to demonstrate how depletion RA signaling via receptor (RAR) disrupts progression early pregnancy. To inhibit RAR during pregnancy, BMS493, an inverse pan‐RAR agonist that prevents RA‐induced differentiation, was administered pregnant mice peri‐implantation period. Attenuation RA/RAR prior results in failure, whereas attenuation after post‐implantation decidual vasculature failure by mid‐gestation. endometrial stromal cell (HESC) decidualization, HESCs decidualized were transfected with silencing RNA specific RARA . Inhibition RA/RARA initiation HESC but not maintenance phenotype. These data show is supports mice, distinct initiation, decidualization vitro.

Language: Английский

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Uterine histomorphological and immunohistochemical investigation during the follicular phase of estrous cycle in Saidi sheep

BMC Veterinary Research, Journal Year: 2025, Volume and Issue: 21(1)

Published: Jan. 13, 2025

Abstract Background Saidi sheep are one of the most important farm animals in Upper Egypt, particularly Assiut governorate. Since they can provide meat, milk, fiber, and skins from low-quality roughages, among economically valuable bred for food Egypt. Regarding breeding, relatively little is known about breed. In mammals, uterus a crucial reproductive organ. Therefore, purpose this work was to further details on histological, histochemical, immunohistochemical analyses superoxide dismutase 2 (SOD2), glutathione reductase (GR), progesterone receptor alpha (PRA) as well terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling assay (TUNEL) during follicular phase estrous cycle sheep. Thus, 11 healthy ewes (38.5 ± 2.03 kg weight) ranging age 5 years were used examine histological changes uterus. Results sheep, uterine immunological picture characterized by epithelial stromal proliferation apoptosis. Leucocytic recruitment (lymphocytes, plasma, mast cells) also observed. Uterine gland adenogenesis, vascular angiogenesis, oxidative marker expression, PRA expression muscles, stroma, epithelium noticeable features phase. Conclusion This study provides new evidence role PRA, SOD2, GR, cells controlling apoptosis estrus cycle. These findings have growing significance understanding key mechanisms that characterize successful reproduction enhancing fertility efficiency Sheep.

Language: Английский

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Molecular Insights into the Corin Function at the Uteroplacental Interface

Placenta, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

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Animal models of postpartum hemorrhage

Lab Animal, Journal Year: 2024, Volume and Issue: 53(4), P. 93 - 106

Published: March 25, 2024

Language: Английский

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Guidelines for in vivo models of developmental programming of cardiovascular risk

AJP Heart and Circulatory Physiology, Journal Year: 2024, Volume and Issue: 327(1), P. H221 - H241

Published: May 31, 2024

Research using animals depends on the generation of offspring for use in experiments or maintenance animal colonies. Although not considered by all, several different factors preceding and during pregnancy, as well lactation, can program various characteristics offspring. Here, we present most common models developmental programming cardiovascular outcomes, important considerations study design, provide guidelines producing reporting rigorous reproducible studies exposed to normal conditions insult. These selection appropriate model that should be reported increase rigor reproducibility while ensuring transparent methods results.

Language: Английский

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New uses for an old technique: live imaging on the slice organ culture to study reproductive processes

Biology of Reproduction, Journal Year: 2024, Volume and Issue: 110(6), P. 1055 - 1064

Published: Feb. 3, 2024

Abstract Reproductive processes are dynamic and involve extensive morphological remodeling cell–cell interactions. Live imaging of organs enhances our understanding how biological occur in real time. Slice culture is a type organ where thick slices collected from an cultured for several days. useful easy-to-implement technique live reproductive events at cellular resolution. Here we describe pipeline on slice to visualize the process urethra closure mouse embryonic penis as proof principle. In combination with genetic reporter mice, nuclear stains, exposure experiments, demonstrate feasibility organ. We also provide step-by-step protocol troubleshooting guide facilitate adoption other organs. Lastly, discuss potential utilities experiments that could be implemented sciences.

Language: Английский

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