Low‐Frequency Stimulation at the Ventromedial Hypothalamus Exhibits Broad‐Spectrum Efficacy Across Models of Epilepsy

CNS Neuroscience & Therapeutics, Journal Year: 2025, Volume and Issue: 31(2)

Published: Feb. 1, 2025

The limited efficacy and very restricted antiseizure range of current deep brain stimulation (DBS) targets highlight the need to find an optimal target for managing various seizure types. Here, we aimed investigate DBS on ventromedial hypothalamus (VMH) in different types experimental epileptic seizures. was examined models, potential mechanisms were investigated by using vivo calcium signal recording optogenetics. c-fos expression significantly increased glutamatergic neurons VMH (VMHglu) following Then, 1-Hz low-frequency (LFS) at successfully attenuated severities across models epilepsy, including maximal electroshock, pentylenetetrazol, absence seizure, cortical or hippocampal kainic acid-induced acute hippocampal-kindling models. imaging recordings revealed that LFS could inhibit activities VMHglu. Optogenetic inhibition VMHglu mirrored LFS's impact. Further anterograde viral tracing confirmed extensive distributed projections VMHglu, which may compose circuitry basis broad-spectral LFS. These findings demonstrate VMH-LFS is a broad-spectrum treatment approach decreasing activity.

Language: Английский

Beta-Myrcene as a Sedative–Hypnotic Component from Lavender Essential Oil in DL-4-Chlorophenylalanine-Induced-Insomnia Mice

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(9), P. 1161 - 1161

Published: Sept. 1, 2024

With the increasing prevalence of insomnia-related diseases, effective treatment insomnia has become an important health research topic. Lavender (Lavandula angustifolia Mill.) essential oil (LEO) is a commonly used medicine for and neurological disorders. However, neither active components nor its sedative–hypnotic mechanism have been fully discovered. This study aimed to screen main terpenes discover possible LEO through network pharmacology in as well verify our hypothesis mice. The results showed that, LEO’s 15 potential ingredients, beta-myrcene had strong effects serotonergic synaptic pathway according pharmacological prediction. Further, PCPA(DL-4-chlorophenylalanine)-induced mice were treated with one day or seven days. quiet state was increased effectively, hypnotic effect enhanced by anaobarbital sodium prolonging sleep duration, decreasing latency, rate falling asleep. Beta-myrcene reduced damage hypothalamic neuron cells induced PCPA neurotransmitter levels GABA, 5-HT, Glu serum hypothalamus Meanwhile, exerted improvement upregulating relevant genes protein expression pathway. These support merit activity LEO. Beta-myrcene, terpene LEO, may be source properties. It serve good compound future clinical studies on coping insomnia.

Language: Английский