The role of protein shape in multiphasic separation within condensates

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 26, 2024

Abstract Liquid–liquid phase separation (LLPS) of biological macromolecules leads to the formation various membraneless organelles. LLPS can not only form homogenous condensates but also multilayered and multiphase condensates, which mediate complex cellular functions. However, factors that determine topological features are fully understood. Herein, we focused on Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), a major postsynaptic undergoes forms with other proteins, present minimalistic computational model reproduces these LLPS, including two-phase phase-in-phase (PIP) organization. Analyses this revealed competitive binding two types client proteins is required for PIP formation. The formed when CaMKII had high valency short linker length. Such exhibited low surface tension, modular structure, slow diffusion. These properties consistent functions by store information at synaptic level. Thus, modeling reveals new structure–function relationships as memory unit.

Language: Английский

Cell-Based Assays to Assess Neuroprotective Activity

Neuromethods, Journal Year: 2024, Volume and Issue: unknown, P. 207 - 255

Published: Dec. 26, 2024

Language: Английский