Proteomic analysis of serum small extracellular vesicles identifies diagnostic biomarkers for neuroblastoma DOI Creative Commons
Juan Cheng,

Dongrui Ji,

Jing Ma

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: Aug. 20, 2024

Background Neuroblastoma (NB) primarily arises in children who are <10 years of age, and originates from developing sympathetic nervous system, which results tumors adrenal glands and/or ganglia. The diagnosis NB involves a combination laboratory imaging tests, biopsies. Small extracellular vesicles (sEVs) have gained attention as potential biomarkers for various types tumors. Here, we performed proteomic analysis serum sEVs identified NB. Methods Label-free proteomics were the discovery phase. A bulk RNA-seq dataset tissues was used to analyze association between genes encoding proteins prognosis. Potential validated via multiple reaction monitoring (MRM) or western blot validation public single-cell (scRNA-seq) integrated tissue origin harboring biomarkers. Results total 104 differentially expressed patients with label-free proteomics, 26 MRM analysis. Seven BSG, HSP90AB1, SLC44A1, CHGA, ATP6V0A1, ITGAL SELL showed strong ability distinguish healthy controls non-NB well. Integrated scRNA-seq revealed that these sEVs-derived originated different cell populations tumor tissues. Conclusion sEVs-based may aid molecular NB, representing an innovative strategy improve detection management.

Language: Английский

Ovarian Cancer Screening: Where Do We Stand Now ? DOI Creative Commons
Ikuo Konishi,

Kaoru Abiko,

Takuma Hayashi

et al.

Academia oncology., Journal Year: 2025, Volume and Issue: 2(1)

Published: Jan. 13, 2025

Language: Английский

Citations

1
Oxygen/sulfate radicals-generating CaS2O8 nanosonosensitizers induce PANoptosis and calcium overload for enhanced peritoneal metastasis immunotherapy DOI
Qiang Wang,

Yingfei Wen,

Bo Bi

et al.

Science Bulletin, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

1
A noncanonical role of SAT1 enables anchorage independence and peritoneal metastasis in ovarian cancer DOI Creative Commons
Cuimiao Zheng, Gang Niu, Hao Tan

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 3, 2025

Language: Английский

Citations

0
Extracellular Vesicles: Hermes Between Cancers and Lymph Nodes DOI
Lei‐Ming Cao, Yuan-ye Qiu, Zi‐Zhan Li

et al.

Cancer Letters, Journal Year: 2025, Volume and Issue: unknown, P. 217735 - 217735

Published: April 1, 2025

Language: Английский

Citations

0
Neuron-Derived Extracellular Vesicles: Emerging Regulators in Central Nervous System Disease Progression DOI
Sitong Liu,

A. S. Feng,

Zhigang Li

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Language: Английский

Citations

0
Key techniques for studying extracellular vesicles DOI Open Access

V. V. Kostina,

А. А. Yakovlev

Translational Medicine, Journal Year: 2025, Volume and Issue: 12(1), P. 51 - 66

Published: May 7, 2025

Extracellular vesicles (EVs) are heterogeneous nanoparticles (30–500 nm) found in all biological fluids. The composition of EVs largely reflects the cells that secreted them. At present, widely used to search for diagnostic and therapeutic markers, therefore studies properties becoming more demand today. Despite obvious potential biomedical applications, lack unified methods their isolation, analysis quantitative measurement significantly limits scientific progress this field. In literature there descriptions dozens isolation study EVs, often researchers do not present comparative advantages disadvantages used. regard, a review becomes relevant. This presents comprehensive modern EVs. Each method has its own limitations affecting yield, purity characteristics isolated vesicles, as well accuracy results obtained. choice should be made considering purpose specificity study, since is no universal approach at present. A thorough understanding methodological nuances critical optimizing reproducibility reliability EV research.

Language: Английский

Citations

0
Oncoviral Infections and Small Extracellular Vesicles DOI Creative Commons

Łukasz Ważny,

Theresa L. Whiteside, Monika Pietrowska

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(8), P. 1291 - 1291

Published: Aug. 13, 2024

Small extracellular vesicles (sEV) are small membrane-bound nanovesicles with a size range below 200 nm that released by all types of cells. sEV carry diverse cargo proteins, lipids, glycans, and nucleic acids mimic the content producer mediate intercellular communication play key role in broad variety physiological pathological conditions. Recently, numerous reports have emerged examining viral infections. A significant number similarities biogenesis pathways replication cycles viruses suggest might influence course infections ways. Besides directly modulating virus propagation transporting (complete virions, RNA, DNA), can also modify host antiviral response increase susceptibility cells to infection. The network mutual interactions is particularly complex case oncogenic viruses, deserving special consideration because its significance cancer progression. This review summarizes current knowledge between focusing on abilities modulate carcinogenic properties oncoviruses.

Language: Английский

Citations

2
Proteome-Wide Mendelian Randomization Analysis to Identify Potential Plasma Biomarkers and Therapeutic Targets for Epithelial Ovarian Cancer Subtypes DOI Creative Commons

Qianhan Lin,

Jiajia Li, Yating Sun

et al.

International Journal of Women s Health, Journal Year: 2024, Volume and Issue: Volume 16, P. 2263 - 2279

Published: Dec. 1, 2024

Epithelial ovarian cancer (EOC) remains an unmet medical challenge due to its insidious onset, atypical symptoms, and increasing resistance conventional chemotherapeutic agents. It is imperative explore novel biomarkers generate innovative target drugs.

Language: Английский

Citations

1
Proteomic analysis of serum small extracellular vesicles identifies diagnostic biomarkers for neuroblastoma DOI Creative Commons
Juan Cheng,

Dongrui Ji,

Jing Ma

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: Aug. 20, 2024

Background Neuroblastoma (NB) primarily arises in children who are <10 years of age, and originates from developing sympathetic nervous system, which results tumors adrenal glands and/or ganglia. The diagnosis NB involves a combination laboratory imaging tests, biopsies. Small extracellular vesicles (sEVs) have gained attention as potential biomarkers for various types tumors. Here, we performed proteomic analysis serum sEVs identified NB. Methods Label-free proteomics were the discovery phase. A bulk RNA-seq dataset tissues was used to analyze association between genes encoding proteins prognosis. Potential validated via multiple reaction monitoring (MRM) or western blot validation public single-cell (scRNA-seq) integrated tissue origin harboring biomarkers. Results total 104 differentially expressed patients with label-free proteomics, 26 MRM analysis. Seven BSG, HSP90AB1, SLC44A1, CHGA, ATP6V0A1, ITGAL SELL showed strong ability distinguish healthy controls non-NB well. Integrated scRNA-seq revealed that these sEVs-derived originated different cell populations tumor tissues. Conclusion sEVs-based may aid molecular NB, representing an innovative strategy improve detection management.

Language: Английский

Citations

0