IRE1-dependent GOLIM4 expression controls protein secretion to modulate glioblastoma cell adhesion and migration DOI Open Access

Ketsia Bakambamba,

Manon Nivet, Chloé Sauzay

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 25, 2024

Abstract One of the main glioblastoma (GB) features is diffuse migration tumor cells within surrounding brain parenchyma, rendering almost impossible complete resection and irradiation, leading to inexorable lethal relapse disease. In past years, we demonstrated that IRE1α (hereafter IRE1), one Endoplasmic Reticulum (ER) stress sensors, plays a key role in GB biology by impacting on immune infiltration, angiogenesis cell migration/invasion, all these being linked an alteration protein secretion. present study, investigated if how IRE1 could regulate functionality secretory machinery identified GOLIM4, Golgi-associated molecule whose expression regulated downstream through transcription XBP1s. Interestingly, GOLIM4 silencing led decreased surface multiple molecules including MHC class I molecules, growth factor receptors (PDGFRA IL13RA2) proteins involved cell-cell adhesion (CD44, CD54, NCAM1), matrix (ITGB1) or (CD90) without their encoding transcripts’ levels. Moreover, phenotypically affected models. Overall, have described novel IRE1/XBP1s/GOLIM4 operon controls secretion specific impacts aggressiveness. Graphical abstract

Language: Английский

Dichotomous roles of ACBD3 in NSCLC growth and metastasis DOI Creative Commons
Xiaochao Tan, Chao‐Liang Wu, Priyam Banerjee

et al.

Oncogene, Journal Year: 2025, Volume and Issue: unknown

Published: April 6, 2025

Abstract Lung cancer continues to be the leading cause of cancer-related deaths globally. Unraveling regulators behind lung growth and its metastatic spread, along with understanding underlying mechanisms, is crucial for developing novel effective therapeutic strategies. While much research has focused on identifying potential oncogenes or tumor suppressors, roles certain genes can vary depending context may even exhibit contradictory effects. In this study, we demonstrate that acyl-CoA binding domain containing 3 (ACBD3), a Golgi resident protein, promotes primary by recruiting phosphatidylinositol (PI)-4-kinase IIIβ (PI4KB) Golgi, thereby enhancing oncogenic secretion in chromosome 1q-amplified cells. Conversely, 1q-diploid cells, ACBD3 acts as suppressor metastasis inhibiting NOTCH signaling pathway reducing cell motility. This highlights intricacy progression cautions against simplistic approaches targeting individual therapy.

Language: Английский

Citations

0
Targeting the secretory program of 3q-amplified lung cancers DOI Creative Commons
Luis A. Pardo, Jim C. Norman

Journal of Clinical Investigation, Journal Year: 2024, Volume and Issue: 134(12)

Published: June 16, 2024

Designing strategies to target cell proliferation has been a priority of cancer researchers for decades. However, targeting the secretory programs transformed cells can influence other features such as survival, migration, and communication with tumor stroma. In this issue JCI, Tan colleagues describe functional cooperativity between Golgi-resident proteins Golgi integral membrane protein 4 (GOLIM4) ATPase pathway Ca2+ transporting 1 (ATP2C1) in coordination program 3q-amplified cancers. Targeting these tumors manganese (Mn2+) promoted GOLIM4 degradation imposed blockade that impaired progression stromal recruitment mice. These findings highlight therapeutic malignancies provide promising strategy treat progression.

Language: Английский

Citations

0
The cancer-associated secretory phenotype: a new frontier in targeted therapeutics DOI Creative Commons
Xiaochao Tan, Guan-Yu Xiao, Priyam Banerjee

et al.

Journal of Clinical Investigation, Journal Year: 2024, Volume and Issue: 134(17)

Published: Sept. 2, 2024

Language: Английский

Citations

0
IRE1-dependent GOLIM4 expression controls protein secretion to modulate glioblastoma cell adhesion and migration DOI Open Access

Ketsia Bakambamba,

Manon Nivet, Chloé Sauzay

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 25, 2024

Abstract One of the main glioblastoma (GB) features is diffuse migration tumor cells within surrounding brain parenchyma, rendering almost impossible complete resection and irradiation, leading to inexorable lethal relapse disease. In past years, we demonstrated that IRE1α (hereafter IRE1), one Endoplasmic Reticulum (ER) stress sensors, plays a key role in GB biology by impacting on immune infiltration, angiogenesis cell migration/invasion, all these being linked an alteration protein secretion. present study, investigated if how IRE1 could regulate functionality secretory machinery identified GOLIM4, Golgi-associated molecule whose expression regulated downstream through transcription XBP1s. Interestingly, GOLIM4 silencing led decreased surface multiple molecules including MHC class I molecules, growth factor receptors (PDGFRA IL13RA2) proteins involved cell-cell adhesion (CD44, CD54, NCAM1), matrix (ITGB1) or (CD90) without their encoding transcripts’ levels. Moreover, phenotypically affected models. Overall, have described novel IRE1/XBP1s/GOLIM4 operon controls secretion specific impacts aggressiveness. Graphical abstract

Language: Английский

Citations

0