Animal models of disease: Achievements and challenges

Methods in cell biology, Journal Year: 2025, Volume and Issue: unknown, P. xv - xxi

Published: Jan. 1, 2025

Language: Английский

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The impact of experimental diabetes on intracerebral haemorrhage. A preclinical study

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 176, P. 116834 - 116834

Published: May 29, 2024

Although diabetes mellitus negatively affects post-ischaemic stroke injury and recovery, its impact on intracerebral haemorrhage (ICH) remains uncertain. This study aimed to investigate the effect of experimental (ED) ICH-induced neurological impairment. Sprague-Dawley rats were induced with ED 2 weeks before ICH induction. Animals randomly assigned four groups: 1)Healthy; 2)ICH; 3)ED; 4)ED-ICH. ED-ICH groups showed similar functional assessment. The group exhibited significantly lower volume compared group, except at 1 mo. oedema/ICH ratio cistern displacement higher in group. Vascular markers revealed greater expression α-SMA (ED ED-ICH) ICH. Conversely, (ED-ICH ICH) levels VEGF healthy groups. An assessment myelin tract integrity an increase fractional anisotropy cryomyelin than Additionally, displayed MOG Olig-2 As for inflammation, MCP-1 Notably, did not aggravate outcome; however, it results ICH-related brain oedema, structure volume. influences cerebral vascularisation vascular thickness, limits inflammatory response attenuates deleterious white matter integrity.

Language: Английский

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Metabolites in the regulatory risk assessment of pesticides in the EU

Frontiers in Toxicology, Journal Year: 2023, Volume and Issue: 5

Published: Dec. 19, 2023

A large majority of chemicals is converted into metabolites through xenobiotic-metabolising enzymes. Metabolites may present a spectrum characteristics varying from similar to vastly different compared with the parent compound in terms both toxicokinetics and toxicodynamics. In pesticide arena, role metabolism increasingly recognised as significant factor particularly for design interpretation mammalian toxicological studies toxicity assessment pesticide/metabolite-associated issues hazard characterization risk purposes, including parts various residues ecotoxicological adversities. This particular relevance that are unique humans comparison found vitro or vivo animal studies, but also disproportionate (quantitative differences) between species. Presence underlie potential concerns. review aims current state-of-the-art comparative research assessment, One Health perspectives, future needs based on experiences gained at European Food Safety Authority.

Language: Английский

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Advancements in Ecotoxicological Assessment of Anticancer Agents: Bridging Sustainability and Innovation in Chemical Testing

Green Analytical Chemistry, Journal Year: 2024, Volume and Issue: 11, P. 100162 - 100162

Published: Oct. 11, 2024

Language: Английский

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Promoting New Approach Methodologies (NAMs) for research on skin color changes in response to environmental stress factors: tobacco and air pollution

Frontiers in Toxicology, Journal Year: 2023, Volume and Issue: 5

Published: Oct. 10, 2023

Aging is one of the most dynamic biological processes in human body and known to carry significant impacts on individuals’ self-esteem. Skin pigmentation a highly heritable trait made possible by complex, strictly controlled cellular molecular mechanisms. Genetic, environmental endocrine factors contribute modulation melanin’s amount, type distribution skin layers. One hallmarks extrinsic aging induced stress alteration constitutive pattern clinically defined as senile lentigines and/or melasma or other pigmentary dyschromias. The complexity pollutants tobacco smoke warrants thorough understanding mechanisms which they impact through repeated long-term exposure. Pre-clinical clinical studies demonstrated that are induce reactive oxygen species (ROS) inflammatory events lead directly indirectly hyperpigmentation. Another mechanistic direction provided Aryl hydrocarbon Receptors (AhR) were shown mediate leading hyperpigmentation response regulation melanogenic enzymes transcription involved melanin biosynthesis pathway. In this context, we will discuss diverse range New Approach Methodologies (NAMs) capable provide insights pathways action support design raw ingredients formulations intended counter their any subsequently needed studies.

Language: Английский

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Preface

Methods in cell biology, Journal Year: 2024, Volume and Issue: unknown, P. xvii - xxiv

Published: Jan. 1, 2024

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Animal models of disease: Achievements and challenges

Methods in cell biology, Journal Year: 2024, Volume and Issue: unknown, P. xv - xxi

Published: Jan. 1, 2024

Language: Английский

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Critical-Size Defect Tibialis Anterior (TA) Muscle Regeneration using Ex-Vivo Mice Hindlimbs Culturing under Dynamic Mechanical Loading

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 18, 2024

Abstract In this study, we introduced an innovative computer-controlled ex vivo mice hindlimb culturing platform operating under dynamic loading, coupled with injectable cell-laden nanofibrous matrix (PNCOL), to investigate tissue response and therapeutic outcomes in critical size defect tibialis anterior (TA) muscle regeneration. The combination of mechanical stimulation cell therapy offers a distinctive opportunity delve into the regenerative rehabilitation field create sustainable solutions musculoskeletal (MSK) application loading on whole hindlimbs increased total bone area marrow suggesting increase periosteal formation resorption endosteal surface. Viability assessments confirmed sustained samples throughout study. Then, effect PNCOL injection regeneration at TA site was evaluated. Histological analyses revealed enhanced PNCOL-treated hindlimbs. Structural analysis through scanning electron microscopy (SEM) showed ECM fibers groups. An cytokine levels conditioned media end experiment changes number proteins role wound healing, WNT, IGF-1 signaling anabolic bone. Similarly, gene expression significant upregulation PAX7, Mrf4, MYF5, TGFβ1 mRNA levels, indicating after treatments. Lastly, immunostaining anti-inflammatory (CD206) Overall, organ platform– maintained functions while treatment promoted reduced inflammation. These findings demonstrated potential multidimensional approaches for enhancing MSK disorders. addition, study aligns growing emphasis minimizing animals used research developing robust sense responsible animal experimentation introducing ex-vivo platform.

Language: Английский

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Editorial

Alternatives to Laboratory Animals, Journal Year: 2023, Volume and Issue: 51(4), P. 215 - 216

Published: June 5, 2023

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Editorial

Alternatives to Laboratory Animals, Journal Year: 2023, Volume and Issue: 51(5), P. 293 - 294

Published: Aug. 31, 2023

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