Translational Stroke Research, Journal Year: 2024, Volume and Issue: unknown
Published: July 29, 2024
Language: Английский
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7557 - 7557
Published: July 10, 2024
Transient ischemic attack (TIA) is an early warning sign of stroke and death, necessitating suitable animal models due to the associated clinical diagnostic challenges. In this study, we developed a TIA model using flexible spatially targeted photothrombosis combined with real-time blood flow imaging feedback. By modulating excitation light wavefront technology, precisely created square spot (50 × 250 µm), at distal middle cerebral artery (dMCA). The use laser speckle contrast (LSCI) provided feedback on ischemia, while was ceased upon reaching complete occlusion. Our results demonstrated that photothrombus formed in dMCA spontaneously recanalized within 10 min (416.8 ± 96.4 s), no sensorimotor deficits or infarction 24 h post-TIA. During acute phase, spreading depression occurred ipsilateral dorsal cortex, leading more severe ischemia collateral circulation establishment synchronized onset narrowing. Post-reperfusion, thrombi were primarily visual disappearing h. changes indicative cortical conditions than diameter changes. method successfully establishes photochemical based dMCA, allowing for dynamic observation control thrombus formation recanalization enabling monitoring impacts during phase TIA.
Language: Английский
Citations
2
Neurophotonics, Journal Year: 2024, Volume and Issue: 11(03)
Published: July 15, 2024
Perturbations in the microcirculatory system have been observed neurological conditions, such as Alzheimer's disease or systemic inflammation. However, changes occurring at level of capillary are difficult to translate biomarkers that could be measured macroscopically.
Language: Английский
Citations
1
Biophysical Reviews, Journal Year: 2023, Volume and Issue: 15(5), P. 1279 - 1286
Published: Sept. 4, 2023
Language: Английский
Citations
3
Journal of Cerebral Blood Flow & Metabolism, Journal Year: 2023, Volume and Issue: 44(5), P. 801 - 816
Published: Nov. 21, 2023
Quantifying anatomical and hemodynamical properties of the brain vasculature in vivo is difficult due to limited spatiotemporal resolution neuroimaging, variability between subjects, bias acquisition techniques. This work introduces a metabolically inspired vascular synthesis algorithm for creating digital representation cortical blood supply humans. Spatial organization segment resistances network were generated. Cortical folding macroscale arterial venous vessels reconstructed from MRI MR angiography. The remaining network, including ensembles representing parenchymal capillary bed, synthesized following mechanistic principle based on hydrodynamic efficiency supply. We evaluated model by comparing its simulated values with healthy human measurements macrovessel velocity phase contrast bed transit times bolus arrival dynamic susceptibility contrast. find that measured reasonably agree relevant neuroimaging observables can be recapitulated silico. provides basis describing testing quantitative aspects cerebrovascular circulation are not directly observable. Future applications such brains include investigation organ-wide effects metabolic pathologies.
Language: Английский
Citations
3
APL Photonics, Journal Year: 2023, Volume and Issue: 8(10)
Published: Oct. 1, 2023
Non-invasive imaging plays a crucial role in diagnosing and studying eye diseases. However, existing photoacoustic ophthalmoscopy (PAOM) techniques mice have limitations due to handling restrictions, suboptimal optical properties, limited availability of light sources, permissible fluence at the retina. This study introduces an innovative approach that utilizes Rose Bengal, contrast agent, enhance PAOM contrast. enables visualization deeper structures, such as choroidal vasculature sclera mouse eye, using visible light. The integration near-infrared-II (NIR-II) coherence tomography provides additional tissue insights into potential NIR-II capabilities. To optimize imaging, we developed cost-effective 3D printable phantom fully tip/tilt platform. solution elevates user-friendly technology, which can be used address pressing research questions concerning several ocular diseases, myopia, glaucoma, and/or age-related macular degeneration future.
Language: Английский
Citations
2
Translational Stroke Research, Journal Year: 2024, Volume and Issue: unknown
Published: July 29, 2024
Language: Английский
Citations
0