Single-cell and spatial transcriptomics analysis reveals that Pros1 oligodendrocytes are involved in endogenous neuroprotection after brainstem stroke

Neurobiology of Disease, Journal Year: 2025, Volume and Issue: unknown, P. 106855 - 106855

Published: March 1, 2025

Brainstem stroke accounts only 7-10 % of all ischemic while it had more morbidity and mortality. As the predominant cellular component nerve tracts, oligodendrocytes might provide some neuroprotection against injury in context brainstem stroke, but underlying mechanism remains unclear. A mouse model was established, single-cell RNA sequencing spatial transcriptomic analysis were performed to elucidate phenotype within this context. Loss led neurological impairment following subsequent proliferation observed. We identified a subcluster Pros1+ oligodendrocytes, designated OLG8 cells. These cells increased number after enriched around peri-infarct zone. derived from oligodendrocyte progenitor cells, process found be regulated by Myo1e. that protected interneurons. Notably, overexpression Myo1e marked reduction infarct volume simultaneously improving recovery function. In conclusion, we novel cell subcluster, alleviated facilitating differentiation Our study provided insight into stroke.

Language: Английский

Adhesion-Related Pathways and Functional Polarization of Astrocytes in Traumatic Brain Injury: Insights from Single-cell RNA Sequencing

NeuroMolecular Medicine, Journal Year: 2025, Volume and Issue: 27(1)

Published: April 27, 2025

Language: Английский