Holding time or fixative formulation has no obvious effect on histology of Porites evermanni and Montipora capitata

Veterinary Pathology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 5, 2025

Collection of coral for histologic examination requires holding samples in seawater a time before they are fixed processing. This could adversely affect the interpretation morphologic changes during examinations. We evaluated microscopic morphology Porites evermanni and Montipora capitata held (0–120 minutes) prior to fixation Z-Fix formulated with raw or artificial seawater. saw no evident effects treatments on morphology. However, among 88 statistical comparisons, after accounting false discovery rate, was associated significant increase degree mucosity basal body walls.

Language: Английский

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Effects of heat stress on Mediterranean endemic and endangered reef-building coral Cladocora caespitosa: study on physiological and histological markers

Hydrobiologia, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

Language: Английский

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A combined diagnostic approach for the investigation of lesions resembling aspergillosis in Caribbean sea fans (Gorgonia spp.)

Veterinary Pathology, Journal Year: 2023, Volume and Issue: 60(5), P. 640 - 651

Published: May 23, 2023

Aspergillosis of gorgonian sea fans is a Caribbean-wide disease characterized by focal, annular purple pigmentation with central tissue loss. We applied holistic diagnostic approach including histopathology and combination culture direct molecular identification fungi to evaluate these lesions the goal determining diversity associated micro-organisms pathology. Biopsies were collected from 14 without gross 44 grossly consistent aspergillosis in shallow fringing reefs St. Kitts. Histologically, loss margin had exposure axis amoebocyte encapsulation abundant mixed micro-organisms. Polyp loss, gastrodermal necrosis, coenenchymal amoebocytosis at lesion interface (purpled area transitioning normal tissue) algae ( n = 21), fungus-like hyphae 20), ciliate protists 16), cyanobacteria 15), labyrinthulomycetes 5), or no 8). Slender, septate hyaline predominated over other morphological categories, but confined little host response than periaxial melanization. Hyphae absent 6 lesioned present 5 control biopsies, questioning their pathogenicity necessary role causation. From cultivation, different isolated identified sequencing nuclear ribosomal internal transcribed spacer region. In addition, 2 primer pairs used nested format increase sensitivity for amplification lesions, thereby circumventing cultivation. Results suggest opportunistic infections requiring longitudinal experimental studies better determine pathogenesis.

Language: Английский

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Cytology in cnidaria using Exaiptasia as a model

Diseases of Aquatic Organisms, Journal Year: 2024, Volume and Issue: 158, P. 37 - 53

Published: Feb. 27, 2024

A need exists for additional methods to examine cnidaria at the cellular level aid our understanding of health, anatomy, and physiology this important group organisms. This is particularly acute given that disease emerging as a major factor in declines ecologically functional groups such corals. Here we describe simple method process cnidarian cells microscopic examination using model organism Exaiptasia. We show has least 18 cell types or structures can be readily distinguished based on defined morphological features. Some these related back anatomic features animal both light microscope ultrastructural level. The cnidome Exaiptasia may more complex than what currently understood. Moreover, cells, including some cnidocytes, phagocytize other endosymbionts. Finally, findings shed morphologic complexity cell-associated microbial aggregates their intimate intracellular associations. tools described here could useful cnidaria.

Language: Английский

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Acclimation period affects tissue condition in the Caribbean staghorn coral, Acropora cervicornis

Coral Reefs, Journal Year: 2024, Volume and Issue: 43(1), P. 165 - 170

Published: Jan. 20, 2024

Language: Английский

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Building living systematic reviews and reporting standards for comparative microscopic analysis of white diseases in hard corals

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(7)

Published: July 1, 2024

Abstract Over the last 4 decades, coral disease research has continued to provide reports of diseases, occurrence and severity outbreaks associated signs. Histology using systematic protocols is a gold standard for microscopic assessment diseases in veterinary medical research, while also providing valuable information on host condition. However, uptake histological analysis remains limited. Increasing reefs as human impacts intensify highlights need understand use histology date research. Here, we apply approach collating, mapping reviewing methods used study with ‘white’ signs (i.e., white diseases) hard taxa map effort this field spanning design, sample processing 33 publications identified between 1984 2022. We find that studies have not uniformly detailed methodologies, terminology reporting description inconsistent studies. Combined these limitations reduce repeatability, limiting capacity researchers compare reports. A primary outcome provision transparent repeatable systematically literature taxa, development recommendations standardised procedures aim increasing addition allowing ongoing comparative through living reviews field.

Language: Английский

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Marine Pollution as a Trigger of Discoloration Phenomenon in The Hard Coral, Pocillopora Species at The Gulf of Aqaba, Red Sea, Egypt: Pathological and Molecular Evidences

Journal of Applied Veterinary Sciences/Journal of Applied Veterinary Sciences , Journal Year: 2024, Volume and Issue: 0(0), P. 0 - 0

Published: Aug. 24, 2024

Hard corals are precious marine creatures that comprise a complex form of symbiosis between symbiont algae and coral holobiont. For decades, have been challenged by disastrous events climatic anthropogenic etiologies. Such interactions resulted in devastating disease episodes among populations worldwide. There is scarcity information about diseases hard the Gulf Aqaba, Red Sea, Egypt. Therefore, current study aims to investigate various this pristine habitat within Sea. Whitening dark green dots were most noticeable morphopathology samples collected from Aqaba. Some human-based pathogens, such as Rothia kristinae, Cupriavidus pauculus, Delftia acidovorans, isolated some examined tissues, while Burkholderia cepacia group was nearby sediment. The final identities above-mentioned bacterial isolates molecularly confirmed using 16S RNA sequence analysis. Pathologically, diseased observed with changes forms tissue losses, degenerative changes, eosinophilic granular amoebocytes/agranular cells infiltration. frequent detection microbial pathogens human origin could suggest deleterious environmental pollution origin. Ultimately, entire existence mostly threatened swiftly erupting well aquatic pollution. Thus, concludes real need for extensive ecological, biological, pathological, immunological studies determine eminent threats propose possible control means better/sustainable populations.

Language: Английский

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Pathology of lesions in corals from the US Virgin Islands after emergence of stony coral tissue loss disease

Coral Reefs, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

Language: Английский

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Pathology of tissue loss in three key gorgonian species in the Mediterranean sea

Journal of Invertebrate Pathology, Journal Year: 2024, Volume and Issue: 207, P. 108197 - 108197

Published: Sept. 13, 2024

Language: Английский

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Special focus issue on invertebrate pathology: A growing discipline requiring veterinary diagnosticians

Veterinary Pathology, Journal Year: 2023, Volume and Issue: 60(5), P. 501 - 502

Published: Aug. 30, 2023

Language: Английский

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