Computational Insight into Biofilm Inhibitory Activity of Ketidocillinone B and C against Pseudomonas aeruginosa: A Computational Study
Abstract
Lymphatic
filariasis
(LF)
remains
a
significant
public
health
challenge,
particularly
in
endemic
regions
where
secondary
bacterial
infections
exacerbate
the
morbidity
associated
with
chronic
lymphedema.
Among
these
infections,
Pseudomonas
aeruginosa
stands
out
due
to
its
biofilm-forming
ability
and
resistance
conventional
antibiotics.
This
study
underscores
importance
of
targeting
P.
LF
patients,
as
biofilm-associated
are
prevalent
wounds,
complicating
treatment
increasing
healthcare
burdens.
Leveraging
molecular
docking
dynamics
simulations,
we
screened
100
fungal
polyketides
against
LasR
PqsR,
quorum-sensing
proteins
critical
biofilm
formation.
Ketidocillinone
B
(Ket
B)
C
C)
emerged
promising
candidates
notable
binding
affinities
-9.3
kcal/mol
−
9.5
LasR,
7.9
8.8
respectively.
Molecular
simulations
revealed
sustained
stability
both
compounds
within
active
sites,
energies
-82.559
kJ/mol
68.680
for
86.855
90.342
PqsR.
Pharmacokinetic
evaluations
indicated
high
gastrointestinal
absorption,
solubility,
favorable
metabolic
profiles,
Ket
exhibiting
clearance
rate
16.306
mL/min/kg
14.881
mL/min/kg.
These
findings
highlight
potential
therapeutic
agents
through
computational
investigation.
Future
experimental
validation
could
help
by
offering
novel
approach
mitigate
complications
this
neglected
tropical
disease
using
KetB
starting
scaffold.
Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown
Published: April 4, 2025
Language: Английский