Inflammatory Biomarkers and Oral Health Disorders as Predictors of Head and Neck Cancer: A Retrospective Longitudinal Study

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2279 - 2279

Published: March 4, 2025

Head and neck cancers (HNCs) are often diagnosed late, leading to poor prognosis. Chronic inflammation, particularly periodontitis, has been linked carcinogenesis, but systemic inflammatory markers remain underexplored. This study was the first examine whether elevated C-reactive protein (CRP) can serve as a cost-effective adjunct in HNC risk assessment, alongside oral health indicators. A retrospective cohort analysed 23,742 hospital records (4833 patients, 2015-2022) from University Hospital of Debrecen. cases were identified using ICD-10 codes, with CRP periodontitis key predictors. Kaplan-Meier survival analysis, log-rank tests, Weibull regression used assess risk, model performance evaluated via AIC/BIC ROC curves. Periodontitis significantly associated (HR 5.99 [1.96-18.30]), while (>15 mg/L) independently increased 4.16 [1.45-12.00]). Females had lower than males 0.06 [0.01-0.50]). may cost-effective, easily accessible biomarker for early detection when combined screening. Integrating inflammation into assessment models could potentially improve diagnosis high-risk populations.

Language: Английский

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Dynamic Changes of Dental Plaque and Saliva Microbiota in OSCC Progression

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Objectives To elucidate the microbial and genomic changes in saliva dental plaque during Oral Squamous Cell Carcinoma (OSCC) progression, to identify potential mechanisms virulence factors involved OSCC pathogenesis. Materials Methods Using metagenomic sequencing, 64 samples from patients at different stages of differentiation were examined. Results The results showed notable differences composition profiles across ecological regions degrees. Notably, relative abundance specific microbes, such as Porphyromonas gingivalis, Fusobacterium nucleatum, Haemophilus parainfluenzae, increased poorly differentiated OSCC. Microbial alpha diversity correlates with tumor T staging. Dental microbiota shows higher specialization, especially tumors. Both types become more stable advanced Genomic analysis reveals stages. Subsequently, functional pathway tracing pathogens reveal oral cancer may promote tumorigenesis by secreting like GAPDH, GspG, AllS, drive initiation progression through interactions. Conclusions is associated altered composition, diversity, plaque. Poorly show factors, implicating them tumorigenesis. Clinical Relevance Understanding could help develop new diagnostic biomarkers therapies targeting microbiota, potentially improving early detection, treatment efficacy, prognosis for patients. Maintaining microbiome homeostasis also prevent cancer.

Language: Английский

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Anti-inflammatory and antimicrobial efficacy of coconut oil for periodontal pathogens: a triple-blind randomized clinical trial

Clinical Oral Investigations, Journal Year: 2025, Volume and Issue: 29(4)

Published: March 14, 2025

Abstract Objectives To evaluate the effect of coconut oil on oral bacteriome and inflammatory response in patients with periodontitis by integrating next-generation sequencing analyses pathogenic bacterial shifts quantification markers, thereby assessing its potential as a natural adjunct to standard nonsurgical periodontal therapy. Materials methods A triple-blind clinical trial was conducted 30 participants diagnosed periodontitis, randomized into 3 groups: (1) oil, (2) chlorhexidine (3) placebo. Saliva gingival crevicular fluid (GCF) samples were collected before treatment, one month after post-non-surgical Bacterial DNA extracted, V3-V4 region 16 S rRNA gene PCR-amplified sequenced using Illumina MiSeq technologies. Inflammatory biomarkers, including Interleukin-6 (IL-6) tumor necrosis factor-alpha (TNF-α), quantified from GCF samples. Results Coconut treatment significantly reduced families such Spirochaetaceae Tannerellaceae while promoting beneficial bacteria Streptococcaceae. At genus species levels, pathogens Tannerella forsythia Treponema denticola along increase Streptococcus . The subgingival microbial dysbiosis index improved both groups. Furthermore, demonstrated reduction IL-6 TNF-α indicating decreased local inflammation. Conclusions modulated microbiome markers suggesting effective Clinical relevance This study highlights oil’s therapy, effectively reducing (IL-6, TNF-α). It offers safe alternative chlorhexidine, balance health.

Language: Английский

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Dental Caries, Periodontal Status, and Lifestyle Connections: Examining the Moderating Effects of Sleep, Smoking, Diet, and Mealtime Routines

Nutrients, Journal Year: 2025, Volume and Issue: 17(6), P. 1079 - 1079

Published: March 19, 2025

Background: Lifestyle habits such as sleep, hygiene, or eating conducts are nowadays suffering from a lack of consistency, and this situation is being linked to systemic mental health consequences. Nevertheless, not enough attention has been paid investigate the plausible relation between lifestyle oral health, hence, investigation aims study possible effects certain on in dental students. Methods: A sample 195 students was gathered basic sociodemographic data (gender, age, nationality), hygiene habits, well related smoking, adherence healthy daily schedule constancy were recorded. Oral with reference decayed, missing, filled teeth, recorded using DMFT index bleeding upon probing (BOP). The Healthy Scale (EVS) utilized record mealtime diet characteristics. Descriptive analysis, Pearson correlations, hierarchical linear regression model, moderation analysis performed. Results: present evidence showed direct indicators. Respect routines (MT), correct sleep (SR) balanced (BD) exert moderator effect caries smoking. Smoking shows positive correlation number teeth BOP. Conclusions: Findings suggest that better status. In addition, diet, can act factor

Language: Английский

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Association between oral microbiome and sleep disorders in U.S. adults: analysis of NHANES database 2009–2012

BMC Oral Health, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 1, 2025

The microbiome, especially the gut contributes to regulation, etiology, and pathogenesis of sleep disorder. However, limited evidence regarding oral microbiome's role in Therefore, this study aimed investigate association between disorder microbial diversity whether microbiota is associated with all-cause mortality people included 4,729 individuals National Health Nutrition Examination Survey (NHANES) from 2009 2012 data until 2019. Sleep was assessed by structured questionnaire. microbiome characterized 16 S ribosomal RNA gene sequencing. Logistic regression models were conducted quantify α-diversity different status controlling for potential confounding variables, principal coordinate analysis along permutational multivariate variance β-diversity. using Cox proportional hazard models. showed that a lower number operational taxonomic units (OTUs) (adjusted odds ratio [aOR] = 0.996; 95% confidence interval [CI] 0.994–0.998), less Faith's phylogenetic (aOR 0.954, CI 0.934–0.975), Shannon–Weiner index 0.854, 0.772–0.944) β-diversity revealed communities two groups, as measured Bray–Curtis dissimilarity (R2 0.358%, P 0.001), unweighted UniFrac distance 0.450%, 0.001) weighted 0.709%, 0.001). Furthermore, OTUs (odds [OR] 0.999; 0.998–0.999; < 0.05), (OR 0.987; 0.975–0.998; 0.924; 0.873–0.979; inverse Simpson 0.553; 0.306–0.997; 0.05) all significant increase risk death participants Intra-population richness, inter-population dispersion, have been linked mortality. Overall, these results will help better understand etiology Further studies are required determine mechanisms underlying

Language: Английский

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