Astragaloside IV promotes cerebral tissue restoration through activating AMPK- mediated microglia polarization in ischemic stroke rats

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 334, P. 118532 - 118532

Published: July 6, 2024

Language: Английский

Multifunctional extracellular vesicles and edaravone-loaded scaffolds for kidney tissue regeneration by activating GDNF/RET pathway

Nano Convergence, Journal Year: 2024, Volume and Issue: 11(1)

Published: Oct. 26, 2024

With the severity of chronic kidney disease worldwide, strategies to recover renal function via tissue regeneration provide alternatives replacement therapy. To exclude side effects from direct cell transplantation, extracellular vesicles (EVs) are great substitutes representing paracrine signaling. build three-dimensional structures for implantation into 5/6 nephrectomy model by incorporating bioactive materials, including multifunctional EVs (mEVs), porous PMEZE/mEV scaffolds were developed in combination with edaravone (EDV; E) and mEV based on PMEZ PLGA (P), MH-RA (M), ECM (E), ZnO-ALA (Z). The oxygen free radical scavenger EDV was incorporated induce tubular regeneration. mEVs engineered serve regenerative activities a two SDF-1α overexpressed tonsil-derived mesenchymal stem cells (sEVs) intermediate mesoderm (IM) during differentiation progenitor (dEVs). displayed beneficial facilitating migration inducing surrounding cells, improved regulating GDNF/RET pathway their downstream genes. promotion MSC recruitment confirmed sEV particles number dependently, regulation effect its enhanced elucidated using vitro analysis. tubules additionally demonstrated through increased expression aquaporin-1 (AQP-1) cadherin-16 (CDH16) proximal tubules, calbindin PAX2 distal defect model. these, structural functional recovery achieved mice

Language: Английский

Development of Salvianolic Acid A‐Loaded Gelatin Nanoparticles for Effective Suppression of Inflammation and Oxidative Stress in Blood–Brain Barrier to Ischemic Stroke Therapy

Biotechnology and Applied Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: June 4, 2025

ABSTRACT Ischemic stroke (ICS) represents a treatment emergency for which efficient therapeutic options remain insufficient. Salvianolic acid A (SAA) is naturally occurring polyphenol recognized as an effective neuroprotective agent. Compared with developed drugs, SAA demonstrates low side effects and displays several modes of action, providing considerable benefits in managing ICS. Yet, limitations inadequate transmembrane permeability water solubility hinder the effectiveness SAA. Recently, nanodelivery methods have garnered significant attention ICS to penetrate blood–brain barrier enhance drug solubility. This investigation new nanomedicine (SAA@GRH NPs) treatment, utilizing SAA‐loaded gelatin nanoparticles (SAA@GNPs) that were functionalized altered brain tissue target rabies virus glycoprotein (RVG29). The stability, antioxidant, antibacterial, effects, cellular uptake, cytocompatibility SAA@GRH NPs examined. vivo efficacy on was studied rat model middle cerebral artery occlusion (MCAO) histological analysis. resultant enhanced demonstrated dispersion. In vitro studies indicate possess antibacterial activities, antioxidant capabilities, protection against intracellular reactive oxygen species. RVG29 markedly improved absorption by IMR32 cells. Moreover, investigations confirmed mitigating nerve injury facilitating neurological recovery. MCAO model, diminished neuroinflammation, substantially recovered behavioral functions decreased neuronal death. Collectively, our data suggested may offer innovative promising strategy successful

Language: Английский