IGF2BP1 Enhances Neprilysin mRNA Stability to Promote Proliferation, Invasion, and Angiogenesis in Placental Trophoblasts DOI Creative Commons
Chunhai Fan, Huanjiao Jenny Zhou, Yan Pan

et al.

International Journal of General Medicine, Journal Year: 2025, Volume and Issue: Volume 18, P. 967 - 980

Published: Feb. 1, 2025

Preeclampsia (PE) is a severe gestational disorder characterized by sudden hypertension and proteinuria, with substantial risks to both mother fetus. This study aims delineate the role of neprilysin, metalloprotease known for its in modulating vasoactive peptides, pathophysiology PE. We recruited cohort 57 participants, comprising 38 patients diagnosed PE 19 healthy controls, matched demographic clinical characteristics. Neprilysin expression was assessed serum placental tissues through quantitative RT-qPCR Western blot analyses. Functional impacts neprilysin modulation were explored via siRNA knockdown overexpression HTR8/SVneo cells, followed assessments oxidative stress, mitochondrial function, apoptosis, trophoblast invasion using various biochemical assays including CCK-8, DCFH-DA, JC-1 staining, flow cytometry. Our results demonstrate marked compared controls. Elevated levels positively correlated increased systolic diastolic blood pressures. In functional assays, alleviated H2O2-induced restored improved cell migration EVT cells. Conversely, IGF2BP1, regulator mRNA stability, exacerbated intensified cellular damage under stress conditions. The reciprocal regulation IGF2BP1 highlights critical molecular interplay impacting resilience These findings establish as mediator pathogenesis PE, where aberrant linked hypertensive symptoms impaired function. interaction between provides potential therapeutic target mitigating progression suggesting avenues future intervention strategies.

Language: Английский

IGF2BP1 Enhances Neprilysin mRNA Stability to Promote Proliferation, Invasion, and Angiogenesis in Placental Trophoblasts DOI Creative Commons
Chunhai Fan, Huanjiao Jenny Zhou, Yan Pan

et al.

International Journal of General Medicine, Journal Year: 2025, Volume and Issue: Volume 18, P. 967 - 980

Published: Feb. 1, 2025

Preeclampsia (PE) is a severe gestational disorder characterized by sudden hypertension and proteinuria, with substantial risks to both mother fetus. This study aims delineate the role of neprilysin, metalloprotease known for its in modulating vasoactive peptides, pathophysiology PE. We recruited cohort 57 participants, comprising 38 patients diagnosed PE 19 healthy controls, matched demographic clinical characteristics. Neprilysin expression was assessed serum placental tissues through quantitative RT-qPCR Western blot analyses. Functional impacts neprilysin modulation were explored via siRNA knockdown overexpression HTR8/SVneo cells, followed assessments oxidative stress, mitochondrial function, apoptosis, trophoblast invasion using various biochemical assays including CCK-8, DCFH-DA, JC-1 staining, flow cytometry. Our results demonstrate marked compared controls. Elevated levels positively correlated increased systolic diastolic blood pressures. In functional assays, alleviated H2O2-induced restored improved cell migration EVT cells. Conversely, IGF2BP1, regulator mRNA stability, exacerbated intensified cellular damage under stress conditions. The reciprocal regulation IGF2BP1 highlights critical molecular interplay impacting resilience These findings establish as mediator pathogenesis PE, where aberrant linked hypertensive symptoms impaired function. interaction between provides potential therapeutic target mitigating progression suggesting avenues future intervention strategies.

Language: Английский

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