Bioinformatics and next generation data analysis reveals the potential role of inflammation in sepsis and its associated complications DOI Open Access
Basavaraj Vastrad, Chanabasayya Vastrad

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 5, 2023

Abstract Sepsis is the leading systemic inflammatory response syndrome in worldwide, yet relatively little known about genes and signaling pathways involved sepsis progression. The current investigation aimed to elucidate potential key candidate its associated complications. Next generation sequencing (NGS) dataset (GSE185263) was downloaded from Gene Expression Omnibus (GEO) database, which included data 348 samples 44 normal control samples. Differentially expressed (DEGs) were identified using t-tests DESeq2 R package. Next, we made use of g:Profiler analyze gene ontology (GO) REACTOME pathway. Then protein-protein interaction (PPI) these DEGs visualized by Cytoscape with Search Tool for Retrieval Interacting Genes (STRING). Furthermore, constructed miRNA-hub regulatory network TF-hub among hub utilizing miRNet NetworkAnalyst online databases tool software. Finally, performed receiver operating characteristic (ROC) curve analysis through pROC package statistical In total, 958 identified, 479 up regulated down regulated. GO results showed that mainly enriched regulation cellular process, stimulus, extracellular matrix organization immune system. PRKN, KIT, FGFR2, GATA3, ERBB3, CDK1, PPARG, H2BC5, H4C4 CDC20 might be Predicted miRNAs (e.g., hsa-mir-548ad-5p hsa-mir-2113) TFs YAP1 TBX5) found significantly correlated conclusion, DEGs, relative pathways, genes, miRNA help understanding molecular mechanisms underlying complications progression provide targets biomarkers

Language: Английский

Extracellular vesicles derived from CD4+ T cells carry DGKK to promote sepsis-induced lung injury by regulating oxidative stress and inflammation DOI Creative Commons
Guo-Wei Tu, Yi Zhang, Jiefei Ma

et al.

Cellular & Molecular Biology Letters, Journal Year: 2023, Volume and Issue: 28(1)

Published: March 23, 2023

Abstract Background Sepsis is an abnormal immune response after infection, wherein the lung most susceptible organ to fail, leading acute injury. To overcome limitations of current therapeutic strategies and develop more specific treatment, inflammatory process, in which T cell-derived extracellular vesicles (EVs) play a central role, should be explored deeply. Methods Liquid chromatography–tandem mass spectrometry was performed for serum EV protein profiling. The diacylglycerol kinase kappa (DGKK) endotoxin contents patients with sepsis-induced injury were measured. Apoptosis, oxidative stress, inflammation A549 cells, bronchoalveolar lavage fluid, tissues mice measured by flow cytometry, biochemical analysis, enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction, western blot. Results DGKK, key regulator (DAG)/protein C (PKC) pathway, exhibited elevated expression EVs showed strong correlation sepsis severity disease progression. DGKK expressed CD4 + cells under regulation NF-κB pathway delivered target including alveolar epithelial cells. produced lymphocytes exerted toxic effects on induce apoptotic cell death, damage, inflammation. In induced cecal ligation puncture, derived from also promoted tissue lungs. These attenuated inhibition PKC NOX4, downstream effectors DAG. Conclusions This approach established mechanism that T-cell-derived carrying triggered apoptosis, inflammation, damage through DAG/PKC/NOX4 pathway. Thus, distribution further investigated

Language: Английский

Citations

12
Epigenetic regulation and post-translational modifications of ferroptosis-related factors in cardiovascular diseases DOI Creative Commons

Chenchen Jing,

Yupeng Wu, Yuzhu Zhang

et al.

Clinical Epigenetics, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 11, 2025

As an important element of the human body, iron participates in numerous physiological and biochemical reactions. In past decade, ferroptosis (a form iron-dependent regulated cell death) has been reported to contribute pathogenesis progression various diseases. The stability cardiomyocytes is crucial for maintenance normal cardiac activity. Ferroptosis detected many cardiovascular diseases (CVDs), including coronary heart disease, myocardial ischemia–reperfusion injury, failure, chemotherapy-induced damage. cardiomyocytes, epigenetic regulation post-translational modifications regulate expression ferroptosis-related factors, maintain homeostasis, participate CVDs. Currently, there no detailed mechanism explain relationship between this review, we provide initial summary core mechanisms with first focus on factors context common We anticipate that new insights into CVDs provided here will inspire development clinical interventions specifically target active sites these reducing harmfulness health.

Language: Английский

Citations

0
Evidence and perspectives on miRNA, circRNA, and lncRNA in myocardial ischemia-reperfusion injury: a bibliometric study DOI Creative Commons

Xiaowen Bo,

Qiuyu Li, Siyuan Chen

et al.

Journal of Cardiothoracic Surgery, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 15, 2025

miRNA, circRNA, and lncRNA play crucial roles in the pathogenesis progression of myocardial ischemia-reperfusion injury (MI/RI). This study aims to provide valuable insights into lncRNA, MI/RI from a bibliometric standpoint, with goal fostering further advancements this area. The relevant literature field was retrieved Science Citation Index Expanded (SCI-E) database within Web Science. "Analyze Results" "Citation Report" functions WOS were utilized compile annual publication citation counts field. Microsoft Office Excel 2019 used organize visualize data. Furthermore, visualization analyses countries/regions, institutions, authors, keywords, references conducted using software CiteSpace. A total 858 publications included for analysis published across 297 journals, Molecular Medicine Reports contributing highest number publications. Researchers 45 countries participated studies field, those China most research hotspots primarily focus on three areas: role MI/RI, their potential as therapeutic targets, biomarkers. Among these, circular RNA, therapy target, inflammatory response, cardiomyocyte ferroptosis are likely emerge emerging trends overall development is rise. compilation area may researchers more assistance selecting directions, ultimately benefiting patients.

Language: Английский

Citations

0
Ankrd1 as a potential biomarker for the transition from acute kidney injury to chronic kidney disease DOI Creative Commons

Hailin Li,

Lemei Hu,

Changqing Zheng

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 7, 2025

Ischemia-reperfusion injury (IRI) is one of the leading causes acute kidney (AKI), predisposing patients to chronic disease (CKD) due maladaptive renal repair. Nevertheless, molecular mechanisms and biomarkers that cause repair remain unclear. In this study, we used single-nucleus RNA sequencing data from GEO database (GSE139107) identify markers during transition AKI CKD caused by IRI. Analysis intercellular crosstalk, trajectory machine learning algorithms revealed hub cell clusters genes. Proximal tubule (PT) cells, especially a new cluster (New PT2), significantly interacted with fibroblasts transition. The expression levels genes were validated using bulk RNA-seq (GSE98622) further confirmed through RT-qPCR immunohistochemical analysis in ischemia-reperfusion (uIRI) mice. Ankrd1, gene New PT2, showed sustained upregulation proximal AKI. Compared sham-operated group, Ankrd1 mice increased at 0.5 days post-reperfusion, peaked day 1, remained elevated up 60 days. This study indicated was positively associated progression may potentially serve as valuable biomarker for transitional process.

Language: Английский

Citations

0
Non-coding RNAs in myocardial ischemia/reperfusion injury and repair DOI
Mingming Pan, Zhixin Li, Xiaohong Wang

et al.

Current Opinion in Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 100825 - 100825

Published: March 1, 2025

Language: Английский

Citations

0
Ferroptosis-related exosomal non-coding RNAs: promising targets in pathogenesis and treatment of non-malignant diseases DOI Creative Commons
Yiping Zhang, Jun Xie

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Feb. 7, 2024

Ferroptosis, an iron-dependent form of programmed cell death, introduces a novel perspective on cellular demise. This study investigates the regulatory network exosomal non-coding RNAs (ncRNAs), including miRNAs, circRNAs, and lncRNAs, in ferroptosis modulation. The primary goal is to examine pathological roles ferroptosis-related ncRNAs, particularly ischemic reperfusion injuries. research reveals intricate molecular interactions governing interplay between ncRNAs ferroptosis, elucidating their diverse different non-malignant contexts. Attention given impact diseases, cardiac, cerebral, liver, kidney injuries, as well lung, wound, neuronal Beyond theoretical exploration, provides insights into potential therapeutic applications, emphasizing significance mesenchymal stem cells (MSCs)-derived exosomes. Findings underscore pivotal role MSC-derived modulating responses related regulation, introducing cutting-edge dimension. recognition emphasizes importance exosomes crucial mediators with broad implications. Insights unveil promising avenues for targeted interventions, capitalizing providing comprehensive foundation future strategies.

Language: Английский

Citations

3
Noncoding RNAs regulating ferroptosis in cardiovascular diseases: novel roles and therapeutic strategies DOI Creative Commons

Changyong Wu,

Suli Bao,

Huang Sun

et al.

Molecular and Cellular Biochemistry, Journal Year: 2023, Volume and Issue: 479(11), P. 2827 - 2841

Published: Dec. 8, 2023

Abstract The morbidity and mortality rates of cardiovascular diseases (CVDs) are increasing; thus, they impose substantial health economic burdens worldwide, effective interventions needed for immediate resolution this issue. Recent studies have suggested that noncoding RNAs (ncRNAs) play critical roles in the occurrence development CVDs potential therapeutic targets novel biomarkers these diseases. Newly discovered modes cell death, including necroptosis, pyroptosis, apoptosis, autophagy-dependent death ferroptosis, also key CVD progression. However, which differs from other aforementioned forms regulated terms morphology, biochemistry inhereditability, is a unique iron-dependent mode nonapoptotic induced by abnormal iron metabolism excessive accumulation lipid peroxides reactive oxygen species (ROS). Increasing evidence has confirmed ncRNA-mediated ferroptosis involved regulating tissue homeostasis CVD-related pathophysiological conditions, such as cardiac ischemia/reperfusion (I/R) injury, myocardial infarction (MI), atrial fibrillation (AF), cardiomyopathy heart failure (HF). In review, we summarize underlying mechanism discuss effects provide ideas strategies.

Language: Английский

Citations

8
Dexmedetomidine alleviates Hypoxia/reoxygenation-induced mitochondrial dysfunction in cardiomyocytes via activation of Sirt3/Prdx3 pathway DOI

Qingyun Tan,

Wenming Dong,

Qing‐Dong Wang

et al.

DARU Journal of Pharmaceutical Sciences, Journal Year: 2024, Volume and Issue: 32(1), P. 189 - 196

Published: Feb. 26, 2024

Language: Английский

Citations

2
Mitochondria-Associated Organelle Crosstalk in Myocardial Ischemia/Reperfusion Injury DOI
Hui Yao,

Yuxin Xie,

Chaoquan Li

et al.

Journal of Cardiovascular Translational Research, Journal Year: 2024, Volume and Issue: 17(5), P. 1106 - 1118

Published: May 28, 2024

Language: Английский

Citations

1
Extravillous trophoblast cell-derived exosomes induce vascular smooth muscle cell apoptosis via a mechanism associated with miR-143-3p DOI
Han-Bo Liu,

Miaojuan Chen,

Fen Ning

et al.

Molecular Human Reproduction, Journal Year: 2023, Volume and Issue: 29(8)

Published: June 27, 2023

Abstract The remodeling of uterine spiral arteries is a complex process requiring the dynamic action various cell types. During early pregnancy, extravillous trophoblast (EVT) cells differentiate and invade vascular wall, replacing smooth muscle (VSMCs). Several in vitro studies have shown that EVT play an important role promoting VSMC apoptosis, however, mechanism underlying this not fully understood. In study, we demonstrated EVT-conditioned media EVT-derived exosomes could induce apoptosis. Through data mining experimental verification, it was exosome miR-143-3p induced apoptosis both VSMCs chorionic plate artery (CPA) model. Furthermore, FAS ligand also expressed on may co-ordinated induction. These clearly mediated by their cargo as well surface presentation FASL. This finding increases our understanding molecular mechanisms regulation during remodeling.

Language: Английский

Citations

3