Caspase-9 and p53 Protein Levels in Cancer Patients after Different Anesthesia Techniques DOI Creative Commons

A. O. Soloviev,

В. Т. Долгих,

О. Н. Новичкова

et al.

General Reanimatology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

The aim of this study was to investigate the changes in caspase-9 and p53 levels as biomarkers pro- anti-apoptotic pathways early postoperative period patients who underwent lung surgery for malignant tumors under different types multimodal or inhalation-intravenous anesthesia. Material Methods. A single-center prospective 22 aged 45-64 years conducted at Omsk Clinical Oncology Early Treatment Prevention Center from January April 2020. participants were divided into two groups. Group 1 received anesthesia, which included sympathetic nerve block prolonged epidural analgesia period. 2 inhalational intravenous anesthesia followed by systemic morphine analgesia. Serum protein measured four time points: before one, twelve, twenty-four hours after surgery. Statistical hypotheses tested using nonparametric (rank) analysis methods. Friedman's ANOVA used compare multiple points, while Wilcoxon test variables between points dependent samples. Mann-Whitney assess differences groups independent P-values < 0.05 considered statistically significant. Results are expressed median ± half interquartile range (Me (LQ-UQ)/2). Results. At point 2, significantly higher group than (P = 0.045). There no significant any other points. Conclusion. lack a difference serum most demonstrates efficacy methods used. Meanwhile, level one hour greater susceptibility without blockade activation apoptotic cell death program.

Language: Английский

ENC1 Promotes the Malignant Progression and Metastasis by Suppressing TRIM21 Mediated Vimentin Degradation in Wilms Tumor DOI

Zuopeng Lu,

Hongjie Gao,

Fan Huang

et al.

Molecular Carcinogenesis, Journal Year: 2025, Volume and Issue: unknown

Published: April 13, 2025

Ectodermal neural cortex 1 (ENC1) is significantly upregulated in various cancers and shows a positive correlation with poor prognosis advanced clinical stages, such as colorectal cancer, endometrial cancer breast cancer. However, the role of ENC1 Wilms tumor (WT) has not been previously reported. In this study, we conducted several vitro functional experiments established xenograft models to confirm oncogenic potential ENC1. The binding proteins were identified through co-immunoprecipitation mass spectrometry screen mechanism malignant progression. Further analysis elucidated by which promotes tumorigenesis. results demonstrated that was overexpressed recurrence samples, elevated expression showing significant negative both overall survival recurrence-free patients. Functionally, oncogenicity assessment cell proliferation, migration, invasion capabilities. Mechanistically, immunoprecipitation spectrometry, Vimentin an interacting protein competed E3 ubiquitin ligase TRIM21 for binding, thereby reducing ubiquitination level enhancing its stability. conclusion, study demonstrates functions novel target disrupting TRIM21-mediated Vimentin, presents insights treatment development prognostic markers.

Language: Английский

Citations

0
Bisphenol a Disrupts Steroidogenesis and Induces Apoptosis in Human Granulosa Cells Cultured In Vitro DOI Open Access
Dominika Celar Šturm, Tadeja Režen, Nina Jančar

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4081 - 4081

Published: April 25, 2025

Bisphenol A (BPA) is a common synthetic chemical compound classified as an endocrine disruptor. It affects multiple physiological systems in the body, including female reproductive system, particularly granulosa cells (GCs) ovaries, where steroidogenesis occurs. This study investigated impact of various BPA concentrations (environmentally relevant 0.001 µM and 0.1 toxicological concentration 100 µM) exposure times (24 72 h) on cell viability counts vitro production estradiol progesterone human GCs collected from waste follicular fluid IVF patients. Gene expression analysis 182 genes associated with apoptosis was performed using PCR arrays, followed by protein Western blot. Our results demonstrate that after longer (72 h), higher (100 negatively cellular significantly alters steroid hormone biosynthesis vitro, leading to reduced culture medium. We found all altered different steroidogenesis- apoptosis-related GCs. At μM, decreased TRIM25, UGT2B15, CASP3, RPS6KA3 increased NR6A1 PPID genes. AR, HSD3B1, BID, IKBKG, while reducing TRIM25 CASP3 highest upregulated GPER30, downregulated FOXO1 UGT2B15 These highlight BPA’s concentration-specific effects show its potential compromise GC function, possible negative implications for fertility ovarian health, even at environmentally concentrations.

Language: Английский

Citations

0
Design, Synthesis, and Biological Evaluation of Newly Synthesized Cinnamide-Fluorinated Containing Compounds as Bioactive Anticancer Agents DOI Creative Commons
Dalal Nasser Binjawhar, Fawziah A. Al‐Salmi, Maha Al‐Ghamdi

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(16), P. 18505 - 18515

Published: April 10, 2024

A new series of cinnamide-fluorinated derivatives has been synthesized and characterized by using different spectroscopic elemental microanalyses methods. All the prepared

Language: Английский

Citations

3
Advancements in Autophagy Modulation for the Management of Oral Disease: A Focus on Drug Targets and Therapeutics DOI Creative Commons
Md. Ataur Rahman, Mushfiq Hassan Shaikh, Rajat Das Gupta

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(11), P. 2645 - 2645

Published: Nov. 19, 2024

Autophagy is an intrinsic breakdown system that recycles organelles and macromolecules, which influences metabolic pathways, differentiation, thereby cell survival. Oral health essential component of integrated well-being, it critical for developing therapeutic interventions to understand the molecular mechanisms underlying maintenance oral homeostasis. However, because complex dynamic relationship between autophagy health, associated treatment modalities have not yet been well elucidated. Determining how affects at level may enhance understanding prevention targeted diseases. At level, hard soft tissues develop interactions epithelial mesenchymal cells. Aging contributes progression various disorders including periodontitis, cancer, periapical lesions during aging. levels decrease with age, thus indicating a possible association In this review, we critically review aspects their significance in context diseases lesions, periodontal conditions, candidiasis. A better its can guide us new preventative strategies management

Language: Английский

Citations

2
Critical roles of lncRNA-mediated autophagy in urologic malignancies DOI Creative Commons

Lifeng Gan,

Liying Zheng,

Junrong Zou

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: June 13, 2024

Urologic oncology is a significant public health concern on global scale. Recent research indicates that long chain non-coding RNAs (lncRNAs) and autophagy play crucial roles in various cancers, including urologic malignancies. This article provides summary of the latest findings, suggesting lncRNA-mediated could either suppress or promote tumors prostate, kidney, bladder cancers. The intricate network involving different lncRNAs, target genes, mediated signaling pathways plays role urological malignancies by modulating autophagic process. Dysregulated expression lncRNAs can disrupt autophagy, leading to tumorigenesis, progression, enhanced resistance therapy. Consequently, targeting particular control serve as dependable diagnostic tool promising prognostic biomarker oncology, while also holding potential an effective therapeutic approach.

Language: Английский

Citations

0
Spatial transcriptomics identifies novelP. aeruginosavirulence factors DOI Creative Commons
Hao Zhou, Oscar Negrón, Serena Abbondante

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 20, 2024

Abstract To holistically unravel the complexity of pathogen-host interactions within infected tissues we leverage a dual spatial transcriptomic approach that, for first time, simultaneously captures expression Pseudomonas aeruginosa genes alongside entire host transcriptome in model ocular infection. This innovative method reveals differential pathogen and host-specific gene patterns across specific anatomical regions generating unified transcriptional map By integrating these data, developed predictive ridge regression trained on images from tissues. The achieved an R² score 0.923 predicting bacterial burden distributions by using features thereby novel biomarkers associated with disease severity. Our analysis revealed complex interplay between P. nutritional requirements protective responses identified metabolite transport proteins autophagy. Among array iron acquisition transcripts that showed significant enrichment at host-pathogen interface, discovered virulence mediator PA2590. study highlights power transcriptomics, particularly combining transcriptomes, to uncover interactions, advance our understanding mechanisms, point druggable molecules.

Language: Английский

Citations

0
TRIM11 modulates sepsis progression by promoting HOXB9 ubiquitination and inducing the NF-κB signaling pathway DOI Creative Commons

Jiaqi Gan,

Wei Zhang, Fei Pan

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 25, 2024

Abstract Aim The purpose of this investigation was to elucidate the functions TRIM11 and HOXB9 in pathogenesis sepsis, focusing on their influence inflammation, apoptosis, NF-κB signaling pathway. Methods Through public databases, TRIM family genes related sepsis were screened, evaluated as a biomarker through ROC analysis. UbiBrowser database screened downstream identified an important target. THP-1 cells stimulated by Lipopolysaccharide (LPS) induce inflammation simulate sepsis. Flow cytometry, Enzyme-linked immunosorbent assay (ELISA), Western blot (WB) experiments used detect changes cell apoptosis rate, apoptosis-related proteins, inflammatory cytokines after silenced. Additionally, we investigated ubiquitination interaction between effects Results Our findings demonstrated that patient samples had elevated levels expression high clinical diagnostic value. Functional showed knockdown significantly alleviated LPS-induced while did opposite. Notably, simultaneous downregulation balanced these responses, suggesting they play key role regulating associated well apoptosis. In addition, regulated pathway reversing HOXB9-induced activation ubiquitination, novel regulatory mechanism Conclusion highlight pathways, providing new insights into treatment.

Language: Английский

Citations

0
A Comprehensive Systematic Review Coupled with an Interacting Network Analysis Identified Candidate Genes and Biological Pathways Related to Bovine Temperament DOI Open Access
Gilberto Ruiz-De-La-Cruz, Thomas H. Welsh, R.D. Randel

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(8), P. 981 - 981

Published: July 25, 2024

Comprehension of the genetic basis temperament has been improved by recent advances in identification genes and variants. However, due to complexity traits, elucidation architecture is incomplete. A systematic review was performed following Preferred Reporting Items for Systematic Reviews Meta-Analyses (PRISMA) statement analyze candidate related bovine temperament, using as population, SNPs exposure, test outcome, principal search terms outcome (PEO) categories define scope search. The results allowed selection 36 articles after removing duplicates filtering relevance. One hundred-two associated with traits were identified. further analyzed construct an interaction network STRING database, resulting 113 nodes 346 interactions 31 new temperament. Notably, main identified SST members Kelch family. displayed pathways different functions such AMPA receptors, hormones, neuronal maintenance, protein signaling, regulation, serotonin synthesis, splicing, ubiquitination activities. These findings demonstrate interconnected biological processes that regulate behavior stress response mammals. This insight now enables our targeted analysis these newly bovines.

Language: Английский

Citations

0
Identification and characterization of chicken TRIM45 and its role as a negative regulator of ALV-J replication in vitro DOI
Jiaxing Wang,

Qiangzhou Wang,

Yuyu Ping

et al.

Avian Pathology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 10

Published: Oct. 17, 2024

Avian leukosis virus subgroup J (ALV-J) is an alpharetrovirus that infects chickens, causing immunosuppression and a decrease in production performance, leading to substantial economic losses the poultry industry. ALV-J also well-known for its oncogenic properties, inducing tumors such as myelomas hemangiomas infected chickens. TRIM45 has been identified potential tumor suppressor, however, relationship between expression infection remains be elucidated.

Language: Английский

Citations

0
TRIM Proteins: Key Regulators of Immunity to Herpesvirus Infection DOI Creative Commons
Zuberwasim Sayyad, Dhiraj Acharya, Michaela U. Gack

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(11), P. 1738 - 1738

Published: Nov. 6, 2024

Herpesviruses are ubiquitous DNA viruses that can establish latency and cause a range of mild to life-threatening diseases in humans. Upon infection, herpesviruses trigger the activation several host antiviral defense programs play critical roles curbing virus replication dissemination. Recent work from many groups has integrated our understanding TRIM (

Language: Английский

Citations

0