Rice Cytochrome P450 Protein CYP71P1 Is Required for Heat Stress Tolerance by Regulating Serotonin Biosynthesis and ROS Homeostasis DOI Creative Commons

Xuantong Lv,

Xunan Zhao,

Fang Wang

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(7), P. 1072 - 1072

Published: April 1, 2025

Heat stress is one of the major factors affecting crop growth and yield. However, molecular mechanisms underlying rice heat tolerance remain largely unclear. In this study, we identified characterized high temperature sensitive 2 (hts2) mutant, which highly susceptible to stress. Map-based cloning revealed that HTS2 encodes a cytochrome P450 protein (CYP71P1) involved in serotonin biosynthesis. ubiquitously expressed across plant tissues shows strong upregulation response The mutation significantly impairs basal synthesis rice, heat-sensitive phenotype hts2 mutant completely rescued by exogenous supplementation. Compared wild type, exhibits reduced antioxidant capacity, leading excessive reactive oxygen species (ROS) accumulation severe oxidative damage, ultimately reducing tolerance. Furthermore, disruption affects shock response, with heat-induced expression HsfA2s their downstream target genes, such as HSP18.0 (heat 18.0) OsAPX2 (ascorbate peroxidase 2), markedly depressed mutant. Our results suggest pivotal role modulating metabolism maintaining ROS homeostasis during stress, offering new perspectives on potential strategies enhance resilience

Language: Английский

Improvements in Tolerance to Heat Stress in Rice via Molecular Mechanisms and Rice Varieties DOI Creative Commons
He Liu,

Yiting Wei,

Saisai Xia

et al.

Agriculture, Journal Year: 2025, Volume and Issue: 15(3), P. 318 - 318

Published: Jan. 31, 2025

Global warming affects crop growth and development, threatening food security. As one of the essential crops, rice is severely affected by high temperature stress, which compromises both its yield quality. Therefore, gaining a deep understanding molecular mechanisms responds to heat stress breeding varieties that are tolerant such crucial for maintaining This review summarizes impacts on quality-related traits at different development stages rice, perception response improvement in heat-tolerant using existing superior alleles/QTLs. We also discuss opportunities challenges creating highly germplasm, providing new ideas insights future varieties.

Language: Английский

Citations

0
Changes in starch structure, function and enzyme activity in early-season indica rice under simulating high temperature and strong light during grain filling DOI
Ruoyu Xiong, Shiqi Yang,

Xiaofeng Ai

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142491 - 142491

Published: March 1, 2025

Language: Английский

Citations

0
Rice Cytochrome P450 Protein CYP71P1 Is Required for Heat Stress Tolerance by Regulating Serotonin Biosynthesis and ROS Homeostasis DOI Creative Commons

Xuantong Lv,

Xunan Zhao,

Fang Wang

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(7), P. 1072 - 1072

Published: April 1, 2025

Heat stress is one of the major factors affecting crop growth and yield. However, molecular mechanisms underlying rice heat tolerance remain largely unclear. In this study, we identified characterized high temperature sensitive 2 (hts2) mutant, which highly susceptible to stress. Map-based cloning revealed that HTS2 encodes a cytochrome P450 protein (CYP71P1) involved in serotonin biosynthesis. ubiquitously expressed across plant tissues shows strong upregulation response The mutation significantly impairs basal synthesis rice, heat-sensitive phenotype hts2 mutant completely rescued by exogenous supplementation. Compared wild type, exhibits reduced antioxidant capacity, leading excessive reactive oxygen species (ROS) accumulation severe oxidative damage, ultimately reducing tolerance. Furthermore, disruption affects shock response, with heat-induced expression HsfA2s their downstream target genes, such as HSP18.0 (heat 18.0) OsAPX2 (ascorbate peroxidase 2), markedly depressed mutant. Our results suggest pivotal role modulating metabolism maintaining ROS homeostasis during stress, offering new perspectives on potential strategies enhance resilience

Language: Английский

Citations

0