Phylogenetic analysis of the caspase family in bivalves: implications for programmed cell death, immune response and development

BMC Genomics, Journal Year: 2021, Volume and Issue: 22(1)

Published: Jan. 25, 2021

Abstract Background Apoptosis is an important process for organism’s innate immune system to respond pathogens, while also allowing cell differentiation and other essential life functions. Caspases are one of the key protease enzymes involved in apoptotic process, however there currently a very limited understanding bivalve caspase diversity function. Results In this work, we investigated presence homologues using combination bioinformatics phylogenetic analyses. We blasted Crassostrea gigas genome identified 35 potential addition already cloned 23 caspases. As such, present information about relationship all caspases relation their homology well-established vertebrate invertebrate Our results reveal unexpected novelty complexity family. Notably, were unable identify direct initiator caspase-9, key-caspase pathway, inflammatory (caspase-1, − 4 or 5) executioner caspases-3, 6, 7. explored fact that bivalves appear possess several unique groups 2 8. Large expansions caspase-3 like (caspase-3A-C), caspase-3/7 group caspase-3/7-like identified, suggesting unusual roles with implications our response common diseases. Furthermore, assessed gene expression two (Cg2A, Cg8B) four (Cg3A, Cg3B, Cg3C, Cg3/7) C. late-larval development during metamorphosis, indicating varies across different developmental stages. Conclusion analysis provides first overview species new insights into diversity, knowledge can be used further investigations pathogens regulation processes.

Language: Английский

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Stage-Specific Transcriptomes of the Mussel Mytilus coruscus Reveals the Developmental Program for the Planktonic to Benthic Transition

Genes, Journal Year: 2023, Volume and Issue: 14(2), P. 287 - 287

Published: Jan. 21, 2023

Many marine invertebrate larvae undergo complex morphological and physiological changes during the planktonic—benthic transition (a.k.a. metamorphosis). In this study, transcriptome analysis of different developmental stages was used to uncover molecular mechanisms underpinning larval settlement metamorphosis mussel, Mytilus coruscus. Analysis highly upregulated differentially expressed genes (DEGs) at pediveliger stage revealed enrichment immune-related genes. The results may indicate that co-opt molecules immune system sense respond external chemical cues neuroendocrine signaling pathways forecast trigger response. upregulation adhesive protein linked byssal thread secretion indicates anchoring capacity required for arises prior metamorphosis. gene expression support a role systems in mussel provide basis future studies disentangle networks biology important lifecycle transformation.

Language: Английский

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Neuroendocrine regulation of the metamorphic transition of giant clams

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742563 - 742563

Published: April 1, 2025

Language: Английский

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BPA-induced disruption of muscle development and larval metamorphosis in the mussel Mytilus coruscus

Aquatic Toxicology, Journal Year: 2025, Volume and Issue: unknown, P. 107368 - 107368

Published: April 1, 2025

Language: Английский

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The unique biology of catch muscles: insights into structure, function, and robotics innovations

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: April 16, 2025

The Review covers the current state of functions, neurotransmitter innervation, structure, and development contractile apparatus unique group catch muscles inherent only to bivalves. In contrast conventional muscles, during contraction relaxation, possess a ability enter holding state, referred as state. latter consists in energy-efficient maintenance long-lasting tension developed by muscle without consuming ATP-derived energy regulated serotonin acetylcholine. Despite molecular mechanism phenomenon still remains unclear, combination experimental data resulting assumptions allow one design new chemically-driven artificial muscles. analysis structure function this work opens way conceptually strategy for biomimetic robotics, including underwater robotics.

Language: Английский

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Comparative transcriptomic analysis revealed dynamic changes of distinct classes of genes during development of the Manila clam (Ruditapes philippinarum)

BMC Genomics, Journal Year: 2022, Volume and Issue: 23(1)

Published: Sept. 29, 2022

Abstract Background The Manila clam Ruditapesphilippinarum is one of the most economically important marine shellfish. However, molecular mechanisms early development in clams are largely unknown. In this study, we collected samples from 13 stages and compared mRNA expression pattern between by RNA-seq techniques. Results We applied technology to embryonic larval identify critical genes pathways involved their biological characteristics. Important associated with different morphologies during fertilized egg, cell division, differentiation, hatching, metamorphosis were identified. detected highest number differentially expressed comparison pediveliger single pipe juvenile stages, which a time when characteristics greatly change metamorphosis. Gene Ontology (GO) enrichment analysis showed that levels microtubule protein-related molecules Rho upregulated GO terms such as ribosome, translation, organelle enriched clam. Kyoto Encyclopedia Genes Genomes pathway foxo, wnt, transforming growth factor-beta significantly development. These results provide insights into at work periods clams. Conclusion transcriptomic data clues underlying larvae. will help improve reproduction

Language: Английский

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Effects of L-arginine on Nitric Oxide Synthesis and Larval Metamorphosis of Mytilus coruscus

Genes, Journal Year: 2023, Volume and Issue: 14(2), P. 450 - 450

Published: Feb. 9, 2023

To investigate the regulatory functions of L-arginine and nitric oxide (NO) on Mytilus coruscus metamorphosis, M. larvae were exposed to an inhibitor synthase (NOS), aminoguanidine hemisulfate (AGH), a substrate for NO synthesis, L-arginine. We observed that levels showed significant increase, this trend continued with treatment. When NOS activity was inhibited, could not synthesize NO, metamorphosis inhibited even in presence On transfecting pediveliger siRNA followed by exposure, we found did produce larval rate significantly increased, suggesting regulates promoting synthesis. Our findings improve our understanding effects marine environmental factors mollusks.

Language: Английский

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Expression Pattern of Nitric Oxide Synthase during Development of the Marine Gastropod Mollusc, Crepidula fornicata

Genes, Journal Year: 2021, Volume and Issue: 12(2), P. 314 - 314

Published: Feb. 22, 2021

Nitric Oxide (NO) plays a key role in the induction of larval metamorphosis several invertebrate phyla. The inhibition NO synthase Crepidula fornicata, molluscan model for evolutionary, developmental, and ecological research, has been demonstrated to block initiation highlighting that endogenous is crucial control this developmental morphological process. Synthase contributes development shell gland, digestive gland kidney, being expressed cells presumably correspond FMRF-amide, serotoninergic catecolaminergic neurons. Here we identified single Nos gene embryonic transcriptomes C. fornicata studied its localization during development, through whole-mount situ hybridization, order compare expression pattern with other marine animal models.

Language: Английский

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Neuroendocrine Regulation of the Metamorphic Transition of Giant Clams

Published: Jan. 1, 2024

Language: Английский

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