Palynology, Journal Year: 2023, Volume and Issue: 48(1)
Published: Sept. 8, 2023
Language: Английский
Journal of Insect Conservation, Journal Year: 2024, Volume and Issue: 28(3), P. 561 - 575
Published: April 15, 2024
Abstract Orchid bee species are important pollinators in the Neotropics. While male orchid bees known to have a close interaction with odor-rewarding flowers, females often pollen generalists. In current study, we investigated differences diet diversity of various vegetation types and across sites varying levels forest cover by means an analysis frass pellets bee, Euglossa cordata (Linnaeus, 1758), multiple based on literature review. The grains found inner part brood cells were used assess plant composition making up E. areas state São Paulo Brazil. We that this differed among types, specialization was lower forested areas. Forest appeared be factor mostly associated richness nests . Number per nest also cover. model effect as tested inclusion published data newly generated for annectans This new set allowed understand influence more extensive geographic scale. diversified several species. Implications insect conservation Since offered larva is survival health, our results indicate relying higher resilient population. Such findings emphasize importance forests, especially Neotropical region where many pollinator adapted continuous environments.
Language: Английский
Citations
4
Journal of Ecology, Journal Year: 2025, Volume and Issue: unknown
Published: March 14, 2025
Abstract Cross‐kingdom associations play a fundamental role in ecological processes. Yet our understanding of plant–fungal co‐occurrences tropical rainforests and the potential impacts land‐use change shaping species connections remain limited. By using amplicon sequencing on DNA from roots their associated fungal communities, we aim to understand impact rainforest transformation composition structure root–fungal networks human‐modified landscapes Sumatra, Indonesia. Each type supports distinctive set indicator species, which are organisms that reflect specific environmental conditions can signal changes ecosystem health. We observed decline richness plant taxa with increasing intensification. Additionally, there is turnover root shifting native endemic non‐native, generalist herbaceous rubber oil palm plantations. Plant–fungal connectivity significantly declined intensification, suggesting managed ecosystems may have weakened root‐fungal interactions. Network analysis highlights distinct responses various groups. For instance, arbuscular mycorrhizal fungi (AMF) showed fewer modules linked roots, indicating monocultures. This aligns reduction AMF diversity converted areas compared forests, further reinforcing negative practices monocultures diversity. Synthesis . Dimensioning transformations below‐ground constrained by functional guilds. Highly modified systems exhibited connections, dynamic restructuring relationships response changes. Understanding intricate interplay between plants face provide valuable information for conservation efforts, agricultural practices, management strategies aimed at promoting biodiversity, soil health resilience context changing conditions. Moreover, it underscores importance communities' planning decisions support terrestrial ecosystems.
Language: Английский
Citations
0
Food Research International, Journal Year: 2025, Volume and Issue: unknown, P. 116399 - 116399
Published: April 1, 2025
Language: Английский
Citations
0
Insectes Sociaux, Journal Year: 2025, Volume and Issue: unknown
Published: April 5, 2025
Language: Английский
Citations
0
Frontiers in Ecology and Evolution, Journal Year: 2023, Volume and Issue: 11
Published: March 14, 2023
The possibility to identify plants based on the taxonomic information coming from their pollen grains offers many applications within various biological disciplines. In past and depending application or research in question, origin was analyzed by microscopy, usually preceded chemical treatment methods. This procedure for identification of is both time-consuming requires expert knowledge morphological features. Additionally, these microscopically recognizable features have a low resolution at species-level. Since few decades, DNA has been used taxa, as sequencing technologies evolved handling affordability. We discuss advantages challenges analyses compared traditional With readers with little experience this field mind, we present hands-on primer genetic analysis nanopore sequencing. As our lab mainly works collected agroecological projects, focus pollinating insects. briefly consider sample collection, storage processing laboratory well bioinformatic aspects. Currently, metabarcoding mostly conducted next-generation methods that generate short sequence reads (<1 kb). Increasingly, however, carried out using long-read generating (several kb), low-budget mobile MinION platform Oxford Nanopore Technologies. Therefore, are focusing aspects palynology device.
Language: Английский
Citations
5
Agriculture Ecosystems & Environment, Journal Year: 2023, Volume and Issue: 353, P. 108552 - 108552
Published: April 26, 2023
Language: Английский
Citations
3
PLoS ONE, Journal Year: 2022, Volume and Issue: 17(12), P. e0277749 - e0277749
Published: Dec. 15, 2022
Molecular biodiversity surveys have been increasingly applied in hyperdiverse tropical regions as an efficient tool for rapid species assessment of partially undiscovered fauna and flora. This is done by overcoming shortfalls knowledge or availability reproductive structures during the sampling period, which often represents a bottleneck accurate specimens’ identification. DNA sequencing technology intensifying discovery, combination with morphological identification, has filling gaps taxonomic facilitating inventories ecosystems. study aimed to apply taxonomy barcoding assess occurrence Lamiaceae converted land-use systems (old-growth forest, jungle rubber, oil palm) Sumatra, Indonesia. In this inventory, we detected 89 specimens from 18 distributed seven subfamilies group. One third identified lacked sequences reference database at least one markers used ( matK , rbcL ITS). The three loci species-tree recovered total 12 out monophyletic lineages can be employed suitable approach molecular assignment Lamiaceae. However, taxa low level interspecific genetic distance barcode study, such Vitex gamosepala Griff. V . vestita Wall. ex Walp., Callicarpa pentandra Roxb. C candidans (Burm.f.) Hochr., use traditional remains indispensable. A change composition decline abundance associated increase intensification family (i.e., Lamiaceae), tendency might constant across other plant families. For reason, maintenance forest resources needs considered sustainable agricultural production, especially regions. Additionally, composition, identification throughout assignments will become more important conservation planning.
Language: Английский
Citations
3
Insects, Journal Year: 2024, Volume and Issue: 15(9), P. 674 - 674
Published: Sept. 5, 2024
The environment significantly impacts the lives of bees and their feeding. This study aimed to investigate bee foraging using melissopalynological analysis DNA metabarcoding in intensive farming, reserved, urbanized areas. highest alpha diversity was observed reserved farming area had less diversity. In area,
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 6, 2024
Abstract Cross-kingdom associations play a fundamental role in ecological processes. Yet our understanding of plant-fungal co-occurrences tropical rainforests and the potential impacts land-use change shaping species connections remains limited. By using amplicon sequencing on DNA from roots their associated fungal communities, we aim to understand impact rainforest transformation composition structure root-fungal networks human-modified landscapes Sumatra, Indonesia. Each type supports distinctive set indicator species, which are organisms that reflect specific environmental conditions can signal changes ecosystem health. We observed decline richness plant indicators with increasing intensification. Additionally, there is turnover root shifting native endemic non-native, generalist herbaceous rubber oil palm plantations. Plant-fungal connectivity significantly declined intensification, suggesting managed ecosystems may have weakened interactions. Network analysis highlights distinct responses various groups. For instance, arbuscular mycorrhizal fungi (AMF) showed fewer modules linked roots, indicating monocultures. This aligns reduction AMF diversity converted areas compared forests, further reinforcing negative practices monocultures diversity. Synthesis. Dimensioning transformations belowground constrained by functional guilds. Highly modified systems exhibited connections, dynamic restructuring relationships response changes. Understanding intricate interplay between plants face provide valuable information for conservation efforts, agricultural practices, management strategies aimed at promoting biodiversity, soil health, resilience context changing conditions. Moreover, it underscores importance communities’ planning decisions support terrestrial ecosystems.
Language: Английский
Citations
0
Diversity, Journal Year: 2023, Volume and Issue: 15(2), P. 279 - 279
Published: Feb. 15, 2023
In endangered crayfish conservation projects, it is paramount to map the distribution of causative agent plague, Aphanomyces astaci, in native populations. Considering inapplicability destructive cuticular sampling protocol for monitoring populations, we explored use non-invasive techniques detect this pathogen with molecular assays. present study, exploited environmental DNA (testing increasing water volumes combined different filter porosities) and swabs collect A. astaci DNA. addition, evaluated impact storage method on preservation during field activities. After first evaluations performed both highly infected Austropotamobius pallipes carrier Procambarus clarkii specimens laboratory conditions, these were applied wild populations white-clawed crayfish. Our findings highlight better results filtration 5 L filters 2.7 µm porosity eDNA analysis demonstrate that swabbing equally effective as World Organisation Animal Health’s protocol. Storage absolute ethanol proved be best solution preserve samples up a week at room temperature. conclusion, suggest an integration methods when purposes.
Language: Английский
Citations
1