
Antibiotics, Journal Year: 2025, Volume and Issue: 14(3), P. 230 - 230
Published: Feb. 24, 2025
Background/Objectives: Pseudomonas aeruginosa causes chronic infections in humans and animals, especially cats dogs. This bacterium’s ability to adapt acquire antibiotic resistance traits may complicate exacerbate antibacterial therapy. study aimed evaluate the patterns, virulence factors form biofilms of P. strains isolated from Algerian dogs cats. Methods: Nineteen samples were collected healthy diseased Isolates studied for their antibiotic-resistance patterns (disc diffusion method) biofilm formation (Microtiter assay) whole-genome sequenced (MinION). Results: (15 4 cats) isolated. Antibiotic-resistance phenotypes observed against amoxicillin–clavulanic acid (100%); meanwhile, towards ticarcillin was 40% (dogs) 25% (cats), ticarcillin–clavulanic 13.33% cats, respectively, imipenem 75% (cats) 20% (dogs). Moreover, 95% biofilm-producers. Different antimicrobial genes (ARGs) found: beta-lactamase genes, mainly PAO, OXA-494, OXA-50 OXA-396 an aminoglycoside gene (aph(3′)-IIb). The main high-risk sequence types (STs) ST244, 2788, 388 1247. A large panel detected: exoS, exoT, exoY, lasA, toxA, prpL, algD, rhIA others. Conclusions: genetic variety resistant virulent makes public health protection difficult. Continuous monitoring research compliance with One Health policy are needed solve this problem.
Language: Английский