A detailed in silico analysis of secondary metabolite biosynthesis clusters in the genome of the broad host range plant pathogenic fungus Sclerotinia sclerotiorum

BMC Genomics, Journal Year: 2020, Volume and Issue: 21(1)

Published: Jan. 2, 2020

The broad host range pathogen Sclerotinia sclerotiorum infects over 400 plant species and causes substantial yield losses in crops worldwide. Secondary metabolites are known to play important roles the virulence of pathogens, but little is about secondary metabolite repertoire S. sclerotiorum. In this study, we predicted biosynthetic gene clusters genome analysed their expression during infection Brassica napus using an existing transcriptome data set. We also investigated sequence diversity among a panel 25 previously published isolate genomes.We identified 80 putative clusters. Over half contained at least three transcriptionally coregulated genes. Comparative genomics revealed homologous closely related Botrytis cinerea for production carotenoids, hydroxamate siderophores, DHN melanin botcinic acid. phytotoxin that can potentially produce polyketide sclerin epipolythiodioxopiperazine. were enriched subtelomeric genomic regions, those containing paralogues showed particularly strong association with repeats. positional bias was borne out by intraspecific comparisons genes suffered more presence / absence polymorphisms exhibited significantly higher than other genes.These suggest produces numerous undergo enhanced rates mutation, duplication recombination regions. microevolutionary regimes leading have yet be elucidated. Several potential phytotoxins documented study provide basis future functional analyses.

Language: Английский

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The evolution of mini-chromosomes in the fungal genus Colletotrichum

mBio, Journal Year: 2023, Volume and Issue: unknown

Published: June 7, 2023

Anthracnose diseases caused by

Language: Английский

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Complete genome of the Medicago anthracnose fungus, Colletotrichum destructivum, reveals a mini-chromosome-like region within a core chromosome

Microbial Genomics, Journal Year: 2024, Volume and Issue: 10(8)

Published: Aug. 21, 2024

Colletotrichum destructivum ( Cd ) is a phytopathogenic fungus causing significant economic losses on forage legume crops Medicago and Trifolium species) worldwide. To gain insights into the genetic basis of fungal virulence host specificity, we sequenced genome an isolate from sativa using long-read (PacBio) technology. The resulting assembly has total length 51.7 Mb comprises ten core chromosomes two accessory chromosomes, all which were telomere to telomere. A 15, 631 gene models predicted, including genes encoding potentially pathogenicity-related proteins such as candidate-secreted effectors (484), secondary metabolism key enzymes (110) carbohydrate-active (619). Synteny analysis revealed extensive structural rearrangements in relative closely related Brassicaceae pathogen, higginsianum . In addition, 1.2 species-specific region was detected within largest chromosome that characteristics (transposon-rich, gene-poor, distinct codon usage), providing evidence for exchange between these genomic compartments. This also unique having undergone intra-chromosomal segmental duplications. Our findings provide evolution regions possible mechanisms generating diversity this asexual pathogen.

Language: Английский

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Pan-Parastagonospora Comparative Genome Analysis—Effector Prediction and Genome Evolution

Genome Biology and Evolution, Journal Year: 2018, Volume and Issue: 10(9), P. 2443 - 2457

Published: Sept. 1, 2018

We report a fungal pan-genome study involving Parastagonospora spp., including 21 isolates of the wheat (Triticum aestivum) pathogen nodorum, 10 grass-infecting avenae, and 2 closely related undefined sister species. observed substantial variation in distribution polymorphisms across pan-genome, repeat-induced point mutations, diversifying selection gene gains losses. also discovered chromosome-scale inter intraspecific presence/absence some sequences, suggesting occurrence one or more accessory chromosomes regions that may play role host-pathogen interactions. The presence known pathogenicity effector loci SnToxA, SnTox1, SnTox3 varied substantially among isolates. Three P. nodorum lacked functional versions for all three loci, whereas avenae carried both SnTox1 genes, indicating previously unrecognized potential discovering additional effectors nodorum-wheat pathosystem. utilized pan-genomic comparative analysis to improve prediction candidates, recovering confirmed our top-ranked candidates. propose applying this approach identify repertoire involved other host-microbe interactions necrotrophic pathogens Pezizomycotina.

Language: Английский

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Chromatin Dynamics Contribute to the Spatiotemporal Expression Pattern of Virulence Genes in a Fungal Plant Pathogen

mBio, Journal Year: 2020, Volume and Issue: 11(5)

Published: Oct. 5, 2020

Dynamic changes in transcription profiles are key for the success of pathogens colonizing their hosts. In many pathogens, genes associated with virulence, such as effector genes, located regions genome that rich transposable elements and heterochromatin. The contribution chromatin modifications to gene expression remains largely unknown. Using a combination reporter gene-based approach immunoprecipitation, we show heterochromatic environment fungal plant pathogen Zymoseptoria tritici is regulator specific spatiotemporal patterns. Enrichment trimethylated lysine 27 histone H3 dictates repression absence host. Chromatin decondensation during host colonization, featuring reduction this repressive modification, indicates major role epigenetics induction. Our results illustrate triggered colonization determine profile at cellular level and, hence, provide new insights into regulation virulence pathogens.IMPORTANCE Fungal possess large repertoire encoding putative effectors, which crucial infection. Many these expressed low levels but strongly induced stages mechanisms underlying transcriptional reprogramming remain We investigated genomic controlling pattern wheat Depending on location, epigenetically repressed initial Derepression occurs mainly after penetration leaves modifications. work demonstrates shaping components thereby, interaction between

Language: Английский

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