Harnessing microbial wealth for lignocellulose biomass valorization through secretomics: a review

Biotechnology for Biofuels, Journal Year: 2021, Volume and Issue: 14(1)

Published: July 5, 2021

Abstract The recalcitrance of lignocellulosic biomass is a major constraint to its high-value use at industrial scale. In nature, microbes play crucial role in degradation, nutrient recycling and ecosystem functioning. Therefore, the an attractive way transform produce clean energy compounds. microbial degradation lignocelluloses complex process which dependent upon multiple secreted enzymes their synergistic activities. availability cutting edge proteomics highly sensitive mass spectrometry tools make possible for researchers probe secretome consortia grown on different identification hydrolytic interest substrate-dependent expression. This review summarizes secretomics identifying involved deconstruction, development enzyme cocktails construction synthetic valorization, providing our perspectives address current challenges.

Language: Английский

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The pan-genome of Aspergillus fumigatus provides a high-resolution view of its population structure revealing high levels of lineage-specific diversity driven by recombination

PLoS Biology, Journal Year: 2022, Volume and Issue: 20(11), P. e3001890 - e3001890

Published: Nov. 17, 2022

Aspergillus fumigatus is a deadly agent of human fungal disease where virulence heterogeneity thought to be at least partially structured by genetic variation between strains. While population genomic analyses based on reference genome alignments offer valuable insights into how gene variants are distributed across populations, these approaches fail capture intraspecific in genes absent from the genome. Pan-genomic de novo assemblies promising alternative reference-based genomics with potential address full repertoire species. Here, we evaluate 260 sequences A . including 62 newly sequenced strains, using combination genomics, phylogenomics, and pan-genomics. Our results high-resolution assessment structure recombination frequency, phylogenetically presence–absence variation, evidence for metabolic specificity, distribution putative antifungal resistance genes. Although disperses primarily via asexual conidia, identified extraordinarily high levels lowest linkage disequilibrium decay value reported any species date. We provide 3 primary populations , occurring only rarely often within them. These both distinct patterns unique suites accessory present exclusively each clade. Accessory displayed functional enrichment nitrogen carbohydrate metabolism suggesting that may stratified environmental niche specialization. Similarly, alleles were phylogeny. Altogether, pan-genome represents one largest pan-genomes date many unrepresented Af293 highlight inadequacy relying single-reference genome-based approach evaluating power combined elucidate structure, diversity, ecological drivers clinically relevant fungi.

Language: Английский

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Developments and opportunities in fungal strain engineering for the production of novel enzymes and enzyme cocktails for plant biomass degradation

Biotechnology Advances, Journal Year: 2019, Volume and Issue: 37(6), P. 107361 - 107361

Published: Feb. 27, 2019

Language: Английский

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Characterization of Thermophilic Lignocellulolytic Microorganisms in Composting

Frontiers in Microbiology, Journal Year: 2021, Volume and Issue: 12

Published: Aug. 11, 2021

Composting involves the selection of a microbiota capable resisting high temperatures generated during process and degrading lignocellulose. A deep understanding thermophilic microbial community involved in such biotransformation is valuable to improve composting efficiency provide thermostable biomass-degrading enzymes for biorefinery. This study investigated lignocellulose-degrading culturome at all stages plant waste composting, focusing on dynamics, enzymes, thermotolerance each member community. The results revealed that 58% holocellulose (cellulose plus hemicellulose) 7% lignin were degraded end composting. whole fungal population exhibited activity, whereas roughly 8-10% bacteria had this trait, although exclusively hemicellulose degradation (xylan-degrading). Because prevalence both groups, their enzymatic wide spectrum thermotolerance, they play key role breakdown entire process, cellulose restricted activity few fungi persists process. xylanolytic bacterial isolates (159 strains) included mostly members Firmicutes (96%) as well representatives Actinobacteria (2%) Proteobacteria (2%). most prevalent species Bacillus licheniformis Aeribacillus pallidus. Thermophilic (27 comprised only four species, namely Thermomyces lanuginosus, Talaromyces thermophilus, Aspergillus fumigatus, Gibellulopsis nigrescens, whom A. fumigatus T. lanuginosus dominated. Several strains same evolved distinctly showing phenotypes with different new enzyme expression, even not previously described response changing environment. Strains thermoamylovorans, Geobacillus thermodenitrificans, exhibiting considerable activities selected potential candidates production thermozymes. lays foundation further investigate mechanisms adaptation acquisition traits among lignocellulolytic microorganisms utility biotechnological processing.

Language: Английский

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Evaluation of Unconventional Protein Secretion by Saccharomyces cerevisiae and other Fungi

Cells, Journal Year: 2018, Volume and Issue: 7(9), P. 128 - 128

Published: Aug. 31, 2018

Development of proteome analysis extracellular proteins has revealed that a wide variety proteins, including fungal allergens are present outside the cell. These secreted often do not contain known secretion signal sequences. Recent research progress shows some by unconventional pathways, autophagy- and extracellular-vesicle-dependent pathways. However, pathways remain unknown for majority proteins. This review summarizes recent data on protein in Saccharomyces cerevisiae other fungi. Particularly, methods evaluating proposed species, S. cerevisiae, popular model organism investigating

Language: Английский

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Epigenetic Molecular Mechanisms in Insects

Neotropical Entomology, Journal Year: 2020, Volume and Issue: 49(5), P. 615 - 642

Published: June 8, 2020

Language: Английский

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Efficient conversion of hemicellulose into 2, 3-butanediol by engineered psychrotrophic Raoultella terrigena: mechanism and efficiency

Bioresource Technology, Journal Year: 2022, Volume and Issue: 359, P. 127453 - 127453

Published: June 11, 2022

Language: Английский

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Bioprospecting CAZymes repertoire of Aspergillus fumigatus for eco-friendly value-added transformations of agro-forest biomass

Biotechnology for Biofuels and Bioproducts, Journal Year: 2024, Volume and Issue: 17(1)

Published: Jan. 3, 2024

Abstract Background Valorizing waste residues is crucial to reaching sustainable development goals and shifting from a linear fossil-based economy circular economy. Fungal cell factories, due their versatility robustness, are instrumental in driving the bio-transformation of residues. The present work isolated potent strain, i.e., Aspergillus fumigatus (ZS_AF), an ancient Złoty Stok gold mine, which showcased distinctive capabilities for efficient hydrolytic enzyme production lignocellulosic wastes. Results study optimized (cellulases, xylanases, β-glucosidases) pine sawdust (PSD) via solid-state fermentation using (ZS_AF). optimization, response surface methodology (RSM), produced twofold increase with maximal yields 119.41 IU/gds CMCase, 1232.23 xylanase, 63.19 β-glucosidase, 31.08 FPase. secretome profiling validated pivotal role carbohydrate-active enzymes (CAZymes) auxiliary biomass valorization. A total 77% were constituted by glycoside hydrolases (66%), carbohydrate esterases (9%), activities (3%), polysaccharide lyases (3%). saccharification pretreated wheat straw PSD generated high reducing sugar 675.36 mg/g 410.15 mg/g, respectively. Conclusion These findings highlight significance efficient, synergistic, cost-effective arsenal fungal valorization potential contribute waste-to-wealth creation through solid-waste management. utilization (ZS_AF) unconventional origin optimization strategies embodies innovative approach that holds propel current methods forward, directing paradigm toward improved efficiency sustainability.

Language: Английский

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Powerful cell wall biomass degradation enzymatic system from saprotrophic Aspergillus fumigatus

The Cell Surface, Journal Year: 2024, Volume and Issue: 11, P. 100126 - 100126

Published: May 21, 2024

Cell wall biomass, Earth's most abundant natural resource, holds significant potential for sustainable biofuel production. Composed of cellulose, hemicellulose, lignin, pectin, and other polymers, the plant cell provides essential structural support to diverse organisms in nature. In contrast, non-plant species like insects, crustaceans, fungi rely on chitin as their primary polysaccharide. The saprophytic fungus

Language: Английский

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Structure of a lytic polysaccharide monooxygenase from Aspergillus fumigatus and an engineered thermostable variant

Carbohydrate Research, Journal Year: 2018, Volume and Issue: 469, P. 55 - 59

Published: Aug. 14, 2018

Language: Английский

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