The synergistic actions of hydrolytic genes reveal the mechanism of Trichoderma harzianum for cellulose degradation

Journal of Biotechnology, Journal Year: 2021, Volume and Issue: 334, P. 1 - 10

Published: May 13, 2021

Language: Английский

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An AA9-LPMO containing a CBM1 domain in Aspergillus nidulans is active on cellulose and cleaves cello-oligosaccharides

AMB Express, Journal Year: 2018, Volume and Issue: 8(1)

Published: Oct. 17, 2018

Lytic polysaccharide monooxygenases (LPMOs) are copper dependent enzymes that carry out oxidative cleavage of cellulose and other polysaccharides. Aspergillus nidulans, an ascomycete fungus contains multiple AA9 LPMOs in the genome, offers excellent model system to study their activity during degradation biomass. AN1602, a dual domain AA9-LPMO A. nidulans appended with carbohydrate-binding module, CBM1, was expressed Pichia pastoris for analyzing on cellulosic substrates. The mass spectral HPAEC analyses showed enzyme cleaves phosphoric acid swollen (PASC) presence reducing agent, yielding range cello-oligosaccharides. In addition polymeric substrate cellulose, AN1602 is also active soluble cellohexaose, property restricted only few characterized LPMOs. Product analysis cleaved cellohexaose revealed C4 sole site oxidation. sequence predicted structure catalytic matched very closely known enzymes.

Language: Английский

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Synergistic action of lytic polysaccharide monooxygenase with glycoside hydrolase for lignocellulosic waste valorization: a review

Biomass Conversion and Biorefinery, Journal Year: 2021, Volume and Issue: 13(10), P. 8727 - 8745

Published: July 23, 2021

Language: Английский

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Accessory enzymes of hypercellulolytic Penicillium funiculosum facilitate complete saccharification of sugarcane bagasse

Biotechnology for Biofuels, Journal Year: 2021, Volume and Issue: 14(1)

Published: Aug. 26, 2021

Abstract Background Sugarcane bagasse (SCB) is an abundant feedstock for second-generation bioethanol production. This complex biomass requires array of carbohydrate active enzymes (CAZymes), mostly from filamentous fungi, its deconstruction to monomeric sugars the production value-added fuels and chemicals. In this study, we evaluated repertoire proteins in secretome a catabolite repressor-deficient strain Penicillium funiculosum , Pf Mig1 88 response SCB induction examined their role saccharification SCB. Results A systematic approach was developed cultivation fungus with aim producing understanding arrays tailored To achieve this, grown media supplemented different concentrations pretreated (0–45 g/L). The profile secreted characterized by enzyme activity assays liquid chromatography–tandem mass spectrometry (LC–MS/MS). total 280 were identified 46% them being clearly as CAZymes. Modulation up 15 g/L led sequential enhancement secretion hemicellulases cell wall-modifying enzymes, including endo-β-1,3(4)-glucanase (GH16), endo-α-1,3-glucanase (GH71), xylanase (GH30), β-xylosidase (GH5), β-1,3-galactosidase (GH43) cutinase (CE5). There ~ 122% 60% increases activities, respectively. also 36% increase activities towards mixed-linked glucans. Induction these improved performance 98% (~ 20% over control), suggesting synergy core cellulases accessing recalcitrant region Conclusion Our findings provide insight into system degradation such highlight importance adding culture medium optimize specific sugarcane bagasse.

Language: Английский

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A thermophilic, pH-tolerant, and highly active GH10 xylanase from Aspergillus fumigatus boosted pre-treated sugarcane bagasse saccharification by cellulases

Industrial Crops and Products, Journal Year: 2021, Volume and Issue: 170, P. 113697 - 113697

Published: June 10, 2021

Language: Английский

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