CRISPR-Cas technology based genome editing for modification of salinity stress tolerance responses in rice (Oryza sativa L.)

Molecular Biology Reports, Journal Year: 2021, Volume and Issue: 48(4), P. 3605 - 3615

Published: April 1, 2021

Language: Английский

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Genome-Wide Identification and Expression Analysis of AP2/ERF Transcription Factor Related to Drought Stress in Cultivated Peanut (Arachis hypogaea L.)

Frontiers in Genetics, Journal Year: 2021, Volume and Issue: 12

Published: Oct. 13, 2021

APETALA2/ethylene response element-binding factor (AP2/ERF) transcription factors (TFs) have been found to regulate plant growth and development various abiotic stresses. However, detailed information of AP2/ERF genes in peanut against drought has not yet performed. Herein, 185 TF members were identified from the cultivated ( A. hypogaea cv. Tifrunner) genome, clustered into five subfamilies: AP2 (APETALA2), ERF (ethylene-responsive-element-binding), DREB (dehydration-responsive-element-binding), RAV (related ABI3/VP), Soloist (few unclassified factors)). Subsequently, phylogenetic relationship, intron–exon structure, chromosomal location AhAP2/ERF further characterized. All these distributed unevenly across 20 chromosomes, 14 tandem 85 segmental duplicated gene pairs which originated ancient duplication events. Gene evolution analysis showed that Tifrunner separated 64.07 66.44 Mya Medicago truncatula L. Glycine max L., respectively. Promoter discovered many cis -acting elements related light, hormones, tissues, stress responsiveness process. The protein interaction network predicted exitance functional among families or subgroups. Expression profiles , dehydration-responsive-element-binding subfamilies significantly upregulated under conditions. Our study laid a foundation provided panel candidate TFs for validation uplift breeding programs drought-resistant cultivars.

Language: Английский

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Genome-Wide Identification and Comprehensive Analysis of the AP2/ERF Gene Family in Pomegranate Fruit Development and Postharvest Preservation

Genes, Journal Year: 2022, Volume and Issue: 13(5), P. 895 - 895

Published: May 17, 2022

Pomegranate (Punica granatum L.) is a kind of fruit with significant economic, ecological and health values. AP2/ERF transcription factors belong to large group mainly found in plants play key roles plant growth development. However, genes pomegranate their implication development postharvest preservation have been little described. In this study, 116 PgAP2/ERF were identified renamed based on chromosomal distributions. Phylogenetic relationship from other species, structures, duplications, annotations, cis-elements promoter sequences, protein-protein interaction networks among proteins comprehensively explored. Expression analysis revealed several PgAP2/ERFs associated the phenotypes seed hardness, including PgAP2/ERF5, PgAP2/ERF36, PgAP2/ERF58, PgAP2/ERF86. Subsequent indicated that many differentially expressed are potentially important regulators Furthermore, expression more than one-half was repressed ‘Tunisian soft seed’ under low-temperature cold storage. The results showed 1-MCP implicated promoting upregulated PgAP2/ERF4, PgAP2/ERF15, PgAP2/ERF26, PgAP2/ERF30, PgAP2/ERF35 PgAP2/ERF45 compared those This indicates these candidate involved preservation. summary, findings present study provide an basis for characterizing family information

Language: Английский

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CtDREB52 transcription factor regulates UV-B-induced flavonoid biosynthesis by transactivating CtMYB and CtF3′H in Safflower (Carthamus tinctorius L.)

Plant Stress, Journal Year: 2024, Volume and Issue: 11, P. 100384 - 100384

Published: Feb. 6, 2024

The APETALA2/ethylene-responsive element binding protein (AP2/ERF) is a highly conserved transcription factors family, involved in plant growth, development, and response to diverse stress conditions. Until recently, the AP2/ERF family has not been functionally characterized safflower. In total, 187 genes were identified from safflower genome. Phylogenetic analysis divided these CtAP2/ERFs into four distinct groups: DREB, ERFs, AP2s, RAV genes. Furthermore, motifs, gene structure, cis-regulatory elements suggested important evolutionary insights potential role of CtAP2/ERFs. Moreover, comprehensive expression conducted across various flowering stages demonstrated consistent pattern CtDREB52, which exhibited significant correlation with alterations total flavonoid content. Importantly, CtDREB52 was found be responsive UV-B stress, its overexpression only promoted accumulation but also induced an early phenotype up-regulated key pathway, while silencing showed opposite effect. Further, interaction between CtDFR CtMYB enzymes confirmed through yeast two-hybrid BiFC analysis. These findings provide CtDREB52-mediated regulation biosynthesis under by interacting

Language: Английский

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Genome-wide identification and expression profiling of AP2/ERF superfamily genes under stress conditions in dark jute (Corchorus olitorius L.)

Industrial Crops and Products, Journal Year: 2021, Volume and Issue: 166, P. 113469 - 113469

Published: April 3, 2021

Language: Английский

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Physiological and Transcriptome Analysis on Diploid and Polyploid Populus ussuriensis Kom. under Salt Stress

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(14), P. 7529 - 7529

Published: July 7, 2022

Populus ussuriensis Kom. is a valuable forest regeneration tree species in the eastern mountainous region of Northeast China. It known that diploid P. (CK) performed barely satisfactorily under salt stress, but stress tolerance polyploid (i.e., triploid (T12) and tetraploid (F20)) still unknown. In order to compare response mechanism between ussuriensis, phenotypic observation, biological biochemistry index detections, transcriptome sequencing (RNA-seq) were on CK, T12, F20. Phenotypic observation leaf injury analysis indicated CK suffered more severe than T12 SOD POD activity detections capacity was stronger MDA content, proline content relative electric conductivity most cell-membrane damage, exhibited strongest osmoprotective stress. Transcriptome DEGs F20 different category change trend, there abundant WRKY, NAM, MYB AP2/ERF genes among GO term enrichment basic growth progresses obviously influenced, while immediately launched processes 36 h after KEGG mainly involved plant−pathogen interaction, ribosome biogenesis eukaryotes, protein processing endoplasmic reticulum, degradation aromatic compounds, plant hormone signal transduction, photosynthesis, carbon metabolism pathways. The cysteine methionine metabolism, phagosomes, biosynthesis amino acids, phenylalanine, tyrosine tryptophan biosynthesis, starch sucrose zeatin glutathione conclusion, > CK). differences probably are key clues for discovering transduction network Ussuriensis.

Language: Английский

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A novel transcription factor, ScAIL1, modulates plant defense responses by targeting DELLA and regulating gibberellin and jasmonic acid signaling in sugarcane

Journal of Experimental Botany, Journal Year: 2022, Volume and Issue: 73(19), P. 6727 - 6743

Published: Aug. 20, 2022

DELLA proteins are important repressors of gibberellin signaling, regulating plant development and defense responses through crosstalk with various phytohormones. Sugarcane ScGAI encodes a protein that regulates culm development. However, it is unclear which transcription factors mediate the ScGAI. Here, we identified two different promoter sequences cooperatively regulate transcription. We also nuclear-localized AP2 family factor, ScAIL1, inhibits by directly binding to promoters. ScAIL1 was expressed in all sugarcane tissues tested induced stressors, including NaCl, polyethylene glycol, pathogenic fungi bacteria. Overexpression rice significantly improved resistance bacterial blight blast, while reducing growth In addition, several genes associated stress were up-regulated transgenic overexpressing ScAIL1. Endogenous phytohormone content expression analysis further revealed ScAIL1-overexpressing lines blast instead promoting growth, this response increased jasmonic acid synthesis inactivation. These results provide molecular evidence role related signaling.

Language: Английский

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Genome-Wide Identification, Evolution, and Expression Analyses of AP2/ERF Family Transcription Factors in Erianthus fulvus

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(8), P. 7102 - 7102

Published: April 12, 2023

The AP2/ERF transcription factor family is one of the most important gene families in plants and plays a vital role plant abiotic stress responses. Although Erianthus fulvus very genetic improvement sugarcane, there are few studies concerning genes E. fulvus. Here, we identified 145 genome. Phylogenetic analysis classified them into five subfamilies. Evolutionary showed that tandem segmental duplication contributed to expansion EfAP2/ERF family. Protein interaction twenty-eight proteins other had potential relationships. Multiple cis-acting elements present promoter were related response, suggesting may contribute adaptation environmental changes. Transcriptomic RT-qPCR analyses revealed EfDREB10, EfDREB11, EfDREB39, EfDREB42, EfDREB44, EfERF43, EfAP2-13 responded cold stress, EfDREB5 EfDREB42 drought EfDREB5, ABA treatment. These results will be helpful for better understanding molecular features biological lay foundation further research on function regulatory mechanism response.

Language: Английский

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Genome-Wide Investigation and Expression Analysis of AP2 Gene Subfamily Reveals Its Evolution and Regulatory Role Under Salt Stress in Populus

Forests, Journal Year: 2025, Volume and Issue: 16(1), P. 94 - 94

Published: Jan. 8, 2025

The APETALA2/ethylene-responsive factor (AP2/ERF) is a well-researched superfamily of plant transcription factors. APETALA2 (AP2) subfamily essential for growth and development. However, systematic analysis the AP2 in poplar has yet to be conducted. This study identified 29 genes genome, classifying them into three clades—euAP2, euANT, basalANT based on evolutionary relationships. These are distributed across 12 chromosomes one scaffold. Results from syntenic suggest that whole-genome duplication events primary factors driving expansion poplar. Cis-element reveals numerous PtAP2 possess hormone-related cis-elements. also contain cis-elements linked development stress responses. PtAP2s different clades exhibit significantly tissue-specific expression patterns Gene levels euAP2 clade higher than euANT various tissues, with showing lowest expression. Through RT-qPCR recombinant Saccharomyces cerevisiae assays under salt stress, it was discovered majority showed negative response regulation trees. In conclusion, this offers valuable insights tolerance trees role conditions.

Language: Английский

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Molecular Mechanisms of Cold Stress Response in Cotton: Transcriptional Reprogramming and Genetic Strategies for Tolerance

Plant Science, Journal Year: 2025, Volume and Issue: unknown, P. 112390 - 112390

Published: Jan. 1, 2025

Language: Английский

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