The class III peroxidase gene family is involved in ascorbic acid induced delay of internal browning in pineapple DOI Creative Commons

Xiaowan Hou,

Zhiwei Lü, Keqian Hong

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Aug. 3, 2022

Excessive production of reactive oxygen species (ROS) leads to potential toxicity in an organism. Class III peroxidases (PRXs) play important role maintaining ROS homeostasis plants. Internal browning (IB) limits industrial development pineapple, which is the third most fruit trade world. IB mainly caused by ROS, and mechanism underlying still unknown from perspective ROS. Here, we soaked pineapples ascorbic acid after harvest before storage decrease excessive polyphenol oxidase (PPO) activity, ultimately restraining spread deterioration IB. Using phylogenetic analysis; identified 78 pineapple PRX genes (AcPRXs) divided them into five subgroups. Gene structure analysis indicated that exon numbers ranged 2 14, conserved motif verified all AcPRXs here have standard peroxidase domains. Analysis duplication events suggested tandem segmental may played equal roles expanding AcPRX family. Comprehensive transcriptomic uncovered negatively regulating occurrence In summary, found scavenging delayed occurrence. The results characterized family revealed responded growth development, regulated stage. This research provides target for future in-depth molecular mechanisms contributes develop IB-resistant varieties.

Language: Английский

Identification and Functional Analysis of Candidate Genes Influencing Citrus Leaf Size Through Transcriptome and Coexpression Network Approaches DOI Open Access
Xiaoxiao Wu, Yuanhui Xiao, Ping Liu

et al.

Genes, Journal Year: 2025, Volume and Issue: 16(1), P. 97 - 97

Published: Jan. 17, 2025

Background: Leaves are the main organs involved in photosynthesis. They capture light energy and promote gas exchange, their size shape affect yield. Identifying regulatory networks key genes that control citrus leaf is essential for increasing crop Methods: In this study, transcriptome sequencing was performed on three materials: ‘Cuimi’ kumquat (Nor) variety its variants, larger-leaf (VarB) smaller-leaf (VarS) varieties. Results: Correlation principal component analyses revealed a relatively close correlation between Nor VarS. A total of 7264 differentially expressed (DEGs), including 2374 transcription factors (TFs), were identified, 254 DEGs common among materials. GO KEGG enrichment significant glucose metabolism, cell wall composition, starch biosynthesis, photosynthesis pathways. WGCNA identified specific modules related to different sizes these Fifteen candidate size, factors, Fh5g30470 (MYB), Fh7g07360 (AP2/ERF), Fh5g02470 (SAP), basis connectivity functional annotations. Conclusions: These findings provide theoretical foundation deeper understanding molecular mechanisms underlying offer new genetic resources study size.

Language: Английский

Citations

0
Transcription Factors in Plant Biotic and Abiotic Stress Responses: Potentials and Prospects in Sugarcane DOI

Aswini Nunavath,

Amaresh,

M. Nandhini

et al.

Tropical Plant Biology, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 31, 2025

Language: Английский

Citations

0
Genome-wide analysis, identification, evolution and genomic organization of dehydration responsive element-binding (DREB) gene family in Solanum tuberosum DOI Creative Commons

Nida Mushtaq,

Faiza Munir, Alvina Gul

et al.

PeerJ, Journal Year: 2021, Volume and Issue: 9, P. e11647 - e11647

Published: June 24, 2021

Background The dehydration responsive element-binding (DREB) gene family plays a crucial role as transcription regulators and enhances plant tolerance to abiotic stresses. Although the DREB has been identified characterized in many plants, knowledge about it Solanum tuberosum (Potato) is limited. Results In present study, StDREB was comprehensively analyzed using bioinformatics approaches. We 66 genes through genome wide screening of Potato based on AP2 domain architecture amino acid conservation analysis (Valine at position 14th). Phylogenetic divided them into six distinct subgroups (A1–A6). categorization further supported by structure conserved motif analysis. were found be distributed unevenly across 12 chromosomes. Gene duplication proved that experienced tandem segmental events which led expansion family. Ka/Ks ratios orthologous pairs also demonstrated under strong purification selection course evolution. Interspecies synteny revealed 45 36 Arabidopsis lycopersicum , respectively. Moreover, subcellular localization indicated predominantly located within nucleus family’s major function DNA binding according ontology (GO) annotation. Conclusions This study provides comprehensive systematic understanding precise molecular mechanism functional characterization stress responses will lead improvement .

Language: Английский

Citations

23
Genome-Wide Identification of APETALA2/ETHYLENE RESPONSIVE FACTOR Transcription Factors in Cucurbita moschata and Their Involvement in Ethylene Response DOI Creative Commons
Qingfei Li, Li Zhang, Peiwen Chen

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: March 15, 2022

APETALA2/ETHYLENE RESPONSIVE FACTOR (AP2/ERF), a plant-specific transcription factor (TF) family, plays an essential role in the growth and development of plants, their response to biotic abiotic stresses. However, information on AP2/ERF Cucurbita moschata (pumpkin), edible medicinal vegetable used worldwide, is scarce. A total 212 genes were identified C. genome (CmoAP2/ERFs). Based phylogenetic analysis, they divided into four groups-28 AP2s, 92 ERFs, 86 dehydration-responsive element-binding (DREB) factors, 6 ABI3/VPs (RAV). The unevenly distributed 20 chromosomes moschata. results structural analysis showed absence introns 132 CmoAP2/ERFs. Four pairs tandem duplication 155 segmental events identified, which indicated that duplications might be main reason for expansion CmoAP2/ERF family. cis-regulatory elements (CREs) most CmoAP2/ERFs contained hormone (ABREs, EREs) promoters, suggesting AP2/ERFs could contribute processes regulated by ethylene abscisic acid. By comparing transcriptome ethephon-treated control we found 16 significantly upregulated after ethephon treatment. Furthermore, determined expression patterns these at different developmental stages female male flowers. This study provides insights identification, classification, physicochemical property, chromosomal location, gene structure, motif CRE prediction superfamily Sixteen as ethylene-inducible genes. this will valuable understanding roles should provide foundation elucidating function TFs

Language: Английский

Citations

15
The class III peroxidase gene family is involved in ascorbic acid induced delay of internal browning in pineapple DOI Creative Commons

Xiaowan Hou,

Zhiwei Lü, Keqian Hong

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Aug. 3, 2022

Excessive production of reactive oxygen species (ROS) leads to potential toxicity in an organism. Class III peroxidases (PRXs) play important role maintaining ROS homeostasis plants. Internal browning (IB) limits industrial development pineapple, which is the third most fruit trade world. IB mainly caused by ROS, and mechanism underlying still unknown from perspective ROS. Here, we soaked pineapples ascorbic acid after harvest before storage decrease excessive polyphenol oxidase (PPO) activity, ultimately restraining spread deterioration IB. Using phylogenetic analysis; identified 78 pineapple PRX genes (AcPRXs) divided them into five subgroups. Gene structure analysis indicated that exon numbers ranged 2 14, conserved motif verified all AcPRXs here have standard peroxidase domains. Analysis duplication events suggested tandem segmental may played equal roles expanding AcPRX family. Comprehensive transcriptomic uncovered negatively regulating occurrence In summary, found scavenging delayed occurrence. The results characterized family revealed responded growth development, regulated stage. This research provides target for future in-depth molecular mechanisms contributes develop IB-resistant varieties.

Language: Английский

Citations

15