Estimating blue mussel (Mytilus edulis) connectivity and settlement capacity in mid-latitude fjord regions

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: Feb. 9, 2024

Abstract The mussel industry faces challenges such as low and inconsistent levels of larvae settlement poor-quality spat, leading to variable production. However, farming remains a vital sustainable environmentally responsible method for producing protein, fostering ecological responsibility in the aquaculture sector. We investigate population connectivity larval dispersion blue mussels ( Mytilus edulis ) Scottish waters, case study, using multidisciplinary approach that combined genetic data particle modelling. This research allows us develop thorough understanding dynamics mid-latitude fjord regions, infer gene-flow patterns, estimate divergence. Our findings reveal primary south-to-north transport direction presence five clusters. discover significant continuous material exchange among populations within study area, with our biophysical model’s outcomes aligning observations. Additionally, model reveals robust connection between southwest coast rest west coast. will guide preservation ensuring contribute marine ecosystem health resilience.

Language: Английский

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Molecular genetic differentiation of native populations of Mediterranean blue mussels,Mytilus galloprovincialisLamarck, 1819, and the relationship with environmental variables

The European Zoological Journal, Journal Year: 2022, Volume and Issue: 89(1), P. 755 - 784

Published: July 14, 2022

Blue mussels of the genus Mytilus are important ecosystem engineers in intertidal and subtidal communities. The distribution is influenced by a number benthic pelagic environmental variables (e.g., substratum type availability, water movement, phytoplankton production, physical disturbance) as well interactions between these variables. Because its broad tolerance variation Mediterranean species, galloprovincialis, has greatest ability all blue to colonise new geographic regions. Understanding how population genetic related to, or caused by, but long been challenge. present study examined differentiation native populations M. galloprovincialis throughout entire range Sea, Black Sea Azov using 53 single nucleotide polymorphisms (SNP loci). Mussels, total 1004 individuals collected from 36 locations, were genotyped combined with existing SNP data for 11 reference sites. Pairwise comparisons FST values, correspondence analysis (CA) STRUCTURE revealed four groups populations: Atlantic Ocean; western Mediterranean; Aegean Sea; Azov, Marmara Seas. One – Algeria (Oran West) was intermediate two main Ocean. Seascape analyses GLM DistLM employed test site-specific function 13 identified five that explained whilst best-fit model only significant. These suggest complex mix contribute explaining within which most likely reflects geological history formation, isolation reconnection among regional sub-basins Sea.

Language: Английский

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Unveiling the Impact of Gene Presence/Absence Variation in Driving Inter-Individual Sequence Diversity within the CRP-I Gene Family in Mytilus spp.

Genes, Journal Year: 2023, Volume and Issue: 14(4), P. 787 - 787

Published: March 24, 2023

Mussels (Mytilus spp.) tolerate infections much better than other species living in the same marine coastal environment thanks to a highly efficient innate immune system, which exploits remarkable diversification of effector molecules involved mucosal and humoral responses. Among these, antimicrobial peptides (AMPs) are subjected massive gene presence/absence variation (PAV), endowing each individual with potentially unique repertoire defense molecules. The unavailability chromosome-scale assembly has so far prevented comprehensive evaluation genomic arrangement AMP-encoding loci, preventing an accurate ascertainment orthology/paralogy relationships among sequence variants. Here, we characterized CRP-I cluster blue mussel Mytilus edulis, includes about 50 paralogous genes pseudogenes, mostly packed small region within chromosome 5. We further reported occurrence widespread PAV this family complex provided evidence that likely adopt knottin fold. functionally synthetic peptide sCRP-I H1, assessing presence biological activities consistent knottins, revealing unlikely act as agents or protease inhibitors, even though they may be used against from eukaryotic parasites.

Language: Английский

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A chromosome-scale Mytilus edulis genome assembly for aquaculture, marine ecology, and evolution

G3 Genes Genomes Genetics, Journal Year: 2024, Volume and Issue: 14(8)

Published: June 27, 2024

Abstract The smooth-shelled blue mussel, Mytilus edulis is part of the species complex, encompassing at least three putative species: M. edulis, galloprovincialis, and trossulus. These occur on both sides Atlantic hybridize in nature, galloprovincialis are important aquaculture species. They also invasive many parts world. Here, we present a chromosome-level assembly edulis. We used combination PacBio sequencing Dovetail's Omni-C technology to generate an with 14 long scaffolds containing 94% predicted length genome (1.6 out 1.7 Gb). Assembly statistics were as follows: total = 1.65 Gb, N50 116 Mb, L50 7, L90 13. BUSCO analysis showed 92.55% eukaryote BUSCOs identified. AB-Initio annotation using RNA-seq from mantle, gills, muscle, foot 47,128 genes. gene models combined IsoSeq validation resulting 45,379 full CDS protein sequences 129,708 isoforms. Using GBS shotgun sequencing, sequenced several eastern Canadian populations characterize single-nucleotide well structural variance. This high-quality for provides platform develop tools that can be breeding, molecular ecology evolution address questions commercial environmental perspectives.

Language: Английский

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Three new genome assemblies of blue mussel lineages: North and South EuropeanMytilus edulisand MediterraneanMytilus galloprovincialis

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Sept. 4, 2022

Abstract The blue mussel species complex ( Mytilus edulls ) is of particular interest both as model in population genetics and ecology, but also an economic resource many regions. Using 10X genomics pseudo-long reads, I assembled genomes three closely related lineages from the Mytllus Northern hemisphere. Given huge diversity within between this complex, objective was to produce affordable genomic resources for evolutionary studies broaden coverage diverse complex. used transcriptome guided corrections scaffolding on a chromosome scale genome close reduce fragmentation genomes. result set partially fragmented equivalent completeness already published Three new draft are added fast increasing Mediterranean M. galloprovlnclalls , South-European North-European .

Language: Английский

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Comparative Analysis of Bivalve and Sea Urchin Genetics and Development: Investigating the Dichotomy in Bilateria

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(24), P. 17163 - 17163

Published: Dec. 5, 2023

This comprehensive review presents a comparative analysis of early embryogenesis in Protostomia and Deuterostomia, the first which exhibit mosaic pattern development, where cells are fated deterministically, while Deuterostomia display regulatory fate is indeterminate. Despite these fundamental differences, there common transcriptional mechanisms that underline their evolutionary linkages, particularly field functional genomics. By elucidating both conserved unique strategies, this provides essential insights into embryology developmental dynamics groups. The objective to clarify shared distinctive characteristics mechanisms. will contribute extensive areas genomics, biology biology, possibly lay foundation for future research discussion on seminal topic.

Language: Английский

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Chromosome-level genome assembly of the disco clam,Ctenoides ales, a first for the bivalve order Limida

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 5, 2024

ABSTRACT The bivalve subclass Pteriomorphia, which includes the economically important scallops, oysters, mussels, and ark clams, exhibits extreme ecological, morphological, behavioral diversity. Among this diversity are five morphologically distinct eye types, making Pteriomorphia an excellent setting to explore molecular basis for evolution of novel traits. Of pteriomorphian bivalves, Limida is only order lacking genomic resources, greatly limiting potential phylogenomic analyses related eyes phototransduction. Here, we present first limid genome assembly, disco clam, Ctenoides ales , characterized by invaginated eyes, exceptionally long tentacles, a flashing light display. This assembly was constructed with PacBio reads Dovetail Omni-C TM proximity-ligation sequencing. final ∼2.3Gb over 99% total length contained in 18 pseudomolecule scaffolds. We annotated 41,064 protein coding genes report BUSCO completeness 91.9% metazoa_obd10. Additionally, completely mitochondrial genome, also Limida. ∼20Kb mitogenome has 12 genes, 22 tRNAs, 2 rRNA 1,589 bp duplicated sequence containing origin replication. C. nuclear size substantially larger than other genomes, mainly accounted transposable element sequences. inventoried opsins, signaling proteins that initiate phototransduction, found that, unlike its closest eyed-relatives, lacks duplication rhabdomeric G q -protein coupled opsin typically used invertebrate vision. In fact, uncharacteristically few opsins relative families, all have unique expansions xenopsins, recently discovered subfamily. chromosome-level along mitogenome, will be valuable resources comparative genomics phylogenetics bivalves particularly understudied but charismatic limids. Significance high-quality sequenced from Limida, group – highly diverse ecologically clade resources. annotation useful resource molluscan genomics.

Language: Английский

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The metatranscriptome of resident microbiota in the gut of blue mussels, Mytilus edulis, under standard laboratory conditions

Current Research in Biotechnology, Journal Year: 2024, Volume and Issue: 7, P. 100208 - 100208

Published: Jan. 1, 2024

Metatranscriptomic methods involving RNA sequencing (RNA-seq) are powerful tools for evaluating the gene expression profiles of transcriptionally-active microbes that inhabit tissues animals. Bivalve molluscs, like all invertebrates, holobionts and sites interactions between host animals both prokaryotic eukaryotic symbionts. The present communication describes metatranscriptomic profile resident microorganisms gut blue mussels, Mytilus edulis, under standard laboratory conditions. Each eight mussels described herein were housed in isolated microcosms with routine husbandry 14 days before their sampled subjected to RNA-seq. Subsequent mRNA reads aligned mussel genome removed, non-host annotated function pathway analyses. Under conditions, microbiota expressed genes associated mostly aerobic energy processes, other notable contributions from metabolism protein processing genes. At level, most abundant complete pathways microbial communities related cellular respiration, nucleotide biosynthesis, catabolism. These data represent novel, baseline information which crucial future research examining holobiont bivalve ecology. Public retrieval secondary analyses these highly encouraged.

Language: Английский

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A chromosome-level genome assembly of the disco clam, Ctenoides ales

G3 Genes Genomes Genetics, Journal Year: 2024, Volume and Issue: 14(9)

Published: May 28, 2024

Abstract The bivalve subclass Pteriomorphia, which includes the economically important scallops, oysters, mussels, and ark clams, exhibits extreme ecological, morphological, behavioral diversity. Among this diversity are five morphologically distinct eye types, making Pteriomorphia an excellent setting to explore molecular basis for evolution of novel traits. Of pteriomorphian bivalves, Limida is only order lacking genomic resources, greatly limiting potential phylogenomic analyses related eyes phototransduction. Here, we present a limid genome assembly, disco clam, Ctenoides ales (C. ales), characterized by invaginated eyes, exceptionally long tentacles, flashing light display. This assembly was constructed with PacBio reads Dovetail Omni-CTM proximity-ligation sequencing. final ∼2.3Gb over 99% total length contained in 18 pseudomolecule scaffolds. We annotated 41,064 protein coding genes reported BUSCO completeness 91.9% metazoa_obd10. Additionally, report complete mitochondrial genome, also had been from Limida. ∼20Kb mitogenome has 12 genes, 22 tRNAs, 2 rRNA 1,589 bp duplicated sequence containing origin replication. C. nuclear size substantially larger than other genomes, mainly accounted transposable element sequences. inventoried opsins, signaling proteins that initiate phototransduction, found that, unlike its closest eyed-relatives, lacks duplication rhabdomeric Gq-protein-coupled opsin typically used invertebrate vision. In fact, uncharacteristically few opsins relative families, all have unique expansions xenopsins, recently discovered subfamily. chromosome-level along mitogenome, valuable resource comparative genomics phylogenetics bivalves particularly understudied but charismatic limids.

Language: Английский

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Insights into ADAR Gene Complement, Expression Patterns, and RNA editing landscape in Chlamys farreri

Fish & Shellfish Immunology, Journal Year: 2024, Volume and Issue: 151, P. 109743 - 109743

Published: July 2, 2024

Language: Английский

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