Evolutionary Dynamics of Lineage-Specific Class-A GPCR Subsets Reveal Widespread Chemosensory Roles and Adaptations in Lophotrochozoa

Published: July 17, 2024

ABSTRACT Detecting external chemosensory cues via Class-A G protein-coupled receptors (GPCRs) is essential for behavioral and biological functions, influencing animal evolution ecological adaptations. While well-studied in vertebrates echinoderms, their role major clades like Lophotrochozoa less understood despite remarkable Utilizing 238 lophotrochozoan genomes across eight phyla, we conducted a large-scale comparative genomics analysis to identify lineage-specifically expanded subsets (LSEs) of GPCRs adapted chemoreception. Using phylogeny orthology-based clustering, differentiated these expansions from conserved orthogroups endogenous ligand-binding GPCRs. LSEs correlated with adaptations diverse habitats, whole-genome duplications having limited impact. Across species coastal, freshwater, terrestrial habitats exhibited large LSEs, while those extreme deep-sea environments, parasitic lifestyles, or alternative mechanisms showed consistent reductions. Sequence heterogeneity, positive selection, pocket flexibility further underscored environmental signals. These findings provide foundational insights into GPCR-mediated chemoreception Lophotrochozoa. Teaser Unveiling correlations between lophotrochozoans habitat lineage-specific changes GPCR repertoire.

Language: Английский

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Transcriptomic responses and evolutionary insights of deep-sea and shallow-water mussels under high hydrostatic pressure condition

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 949, P. 175185 - 175185

Published: July 31, 2024

Language: Английский

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Genome Report: A blue mussel chromosome-scale genome assembly for aquaculture, marine ecology and evolution

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Nov. 18, 2022

Abstract The blue mussel, Mytilus edulis is part of the species complex, encompassing at least three putative species: M. edulis, galloprovincialis and trossulus . These occur on both sides Atlantic hybridize in nature, are important aquaculture species. They also invasive many parts world. Here, we present a chromosome-level assembly We used combination PacBio sequencing Dovetail’s Omni-C technology to generate an with 14 long scaffolds containing 94% predicted length genome (1.6 out 1.7 Gb). Assembly statistics were total 1.65 Gb, N50 = 116 Mb, L50 7 and, L90 13. BUSCO analysis showed 92.55% eukaryote BUSCOs identified. AB- Initio annotation using RNA-seq from mantle, gills, muscle foot 47,128 genes. gene models combined Isoseq validation resulting 65,505 129,708 isoforms. Using GBS shotgun sequencing, sequenced 3 North American populations characterize single-nucleotide as well structural variance. This high-quality for provides platform develop tools that can be breeding, molecular ecology evolution address questions commercial environmental perspectives.

Language: Английский

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Microsatellite Analysis of the Genetic Diversity of Mussels from the North-Western Region of the Black Sea

Hydrobiological Journal, Journal Year: 2023, Volume and Issue: 60(1), P. 54 - 68

Published: Dec. 8, 2023

Using four microsatellite DNA markers (<i>Mch 5, Mch 8, MT 203, 282</i>), the genetic diversity and population structure of three groups mussels from north-western section Black Sea were analyzed, Baltic North Seas studied as outgroups. By (MS) loci, 59 alleles determined in five mussels' (<i>n</i> &#61; 118). The observed heterozygosity (<i>H_O</i>) ranged 0.083 to 0.538 was lower than expected (<i>H_E</i> 0.344-0.858). All mussel showed heterozygote deficiency, which suggests possibility inbreeding. Weak (<i>F_ST</i> 0.036-0.048) moderate 0.054-0.127) differentiation between revealed. Genetic distances different locations calculated, 0.211 1.130. obtained data confirmed by cluster coordinate analyzes carried out using distribution frequencies.

Language: Английский

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