Investigation of genetic diversity in the loach Misgurnus anguillicaudatus revealed by whole-genome resequencing DOI Creative Commons
Xiaojuan Cui,

Jimin Deng,

Yifei Zhang

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Nov. 21, 2024

The loach (Misgurnus anguillicaudatus) is a significant freshwater economic fish in China, renowned for its tender meat, delicious taste, and high nutritional value. It widely distributed across the country, except western plateau. However, currently at risk of population decline degradation wildlife resources. Research on genetic diversity provides crucial foundation conservation development these To enhance protection utilization wild germplasm resources, we analyzed structure 60 populations from Xiangtan City (XT), Shaoyang (SY), Yueyang (XY) Hunan, Guilin (GL) Guiping (GP) Guangxi, Wuhan (WH) Hubei. Additionally, mapped DNA fingerprints loaches using 12 high-quality SNP sites. Whole genome resequencing (WGRS) was conducted six regions, yielding total 1047.17 Gb raw data 1046.98 clean data. From this 2,812,906 single nucleotide polymorphisms (SNPs) were identified, which 10,022 core SNPs selected to analyze structure, used assess fingerprint. analysis revealed that samples could be grouped into three clusters: Cluster A comprised XT, SY, XY groups; B included WH group; C consisted GL GP groups. mean nucleic acid (Pi), heterozygosity (Ho), expected (He) markers 0.130, 0.140, 0.123, respectively. average inbreeding coefficient 0.552, indicating levels inbreeding. an effective high-throughput approach identifying information describing relationships within genus. fingerprinting based marker technology can accurately variation natural populations, making it valuable tool strain identification. Our findings provide scientific basis conservation, development, optimal breeding native resources contribute expanding database populations.

Language: Английский

Transcriptome analysis reveals pituitary lncRNA, circRNA and mRNA affecting fertility in high- and low-yielding goats DOI Creative Commons

Shuaixiang Mao,

Shucan Dong,

Biwei Hou

et al.

Frontiers in Genetics, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 12, 2023

The pituitary gland serves as the central endocrine regulator of growth, reproduction, and metabolism plays a crucial role in reproductive process female animals. Transcriptome analysis was conducted using samples from Leizhou goats with varying levels fecundity to investigate effects long noncoding RNA (lncRNA), circular (circRNA), mRNA regulation on hormone secretion its association goat fecundity. aimed identify lncRNAs, circRNAs, mRNAs that influence fertility goats. GO KEGG enrichment analyses were performed differentially expressed revealed considerable pathways, such secretion, germ cell development, gonadotropin-releasing secretion. lncRNAs (ENSCHIT00000010293, ENSCHIT00000010304, ENSCHIT00000010306, ENSCHIT00000010290, ENSCHIT00000010298, ENSCHIT00000006769, ENSCHIT00000006767, ENSCHIT00000006921, ENSCHIT00000001330) circRNAs (chicirc_029285, chicirc_026618, chicirc_129655, chicirc_018248, chicirc_122554, chicirc_087101, chicirc_078945) identified regulated through their respective host genes. Additionally, differential (GABBR2, SYCP1, HNF4A, CBLN1, CDKN1A) influenced by affecting gland. These findings contribute understanding molecular mechanisms underlying

Language: Английский

Citations

3
Comparison of production performance and meat quality characteristics between Guizhou Black goats and F4 generation hybrids of South African Kalaha Goats DOI Creative Commons
Min Yao,

LingLe CHANG,

SuYun XU

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 18, 2024

Abstract Results (1) Reproductive and production performance: The lambing percentage (LP) reached 196%, significantly higher than GB SK (p < 0.01), with a survival rate of 95.5%; 3-month-old daily weight gain (DWG) was 275.5 g, surpassing 0.01); adult body (ABW) 55.6 kg, slaughter (DP) 53.5%, markedly 0.05). (2) In terms meat quality: BKF4's overall quality falls between SK, two main influencing factors.The key factors affecting include pH45, intramuscular fat (IMF), nutrient index (NI), PUFAs / MUFAs, n-6 FAs, drip loss (DL). water retention, crude protein increased, while content incresed compared to ash decreased. Amino acid composition is similar GB, taste closer SK. Nevertheless, the fatty functionality had negative impacts. Continued improvement will be made through selective breeding livestock management practices like feeding.

Language: Английский

Citations

0
Genome-Wide Association Studies of Growth Trait Heterosis in Crossbred Meat Rabbits DOI Creative Commons

Zhanjun Xiao,

Yuchao Li, Li Yang

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(14), P. 2096 - 2096

Published: July 18, 2024

The application of heterosis can not only effectively improve the disease resistance and meat quality livestock, but also significantly enhance reproduction growth livestock poultry. We conducted genome-wide association studies using data from F2 crossbred rabbits to screen out candidate genes with significant dominant effects associated economic trait variation. High-throughput sequencing technology was used obtain SNPs covering whole genome evaluate homozygosity population genome, analyze number, length, frequency, distribution ROHs in population. Candidate related traits were searched based on high-frequency ROH regions. After control filtering genotype data, 380 identified 78,579 42,018 autosomes. fitting Logistic curve model showed that 49-day-old a inflection point. Then, through studies, 10 SNP loci seven found be body weight at 84 days age. In addition, we revealed functional roles locations 20 region rabbits. This study potential development this study, as molecular markers for assisted selection At same time, inbreeding situation assessment provide reference breeding preservation

Language: Английский

Citations

0
Selection and Regulatory Network Analysis of Differential CircRNAs in the Hypothalamus of Goats with High and Low Reproductive Capacity DOI Open Access

Shuaixiang Mao,

Cuiying Wu,

Guanghang Feng

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10479 - 10479

Published: Sept. 28, 2024

The objectives of this investigation were to identify differentially expressed circular RNAs (circRNAs) in the hypothalamus goats with high and low prolificacy construct a circRNA-mRNA regulatory network uncover key potential circRNAs that influence goat prolificacy. Transcriptome analysis was performed on samples from low-prolificacy (n = 5) high-prolificacy 6) Chuanzhong black these goats. Differential expression identified total 205 circRNAs, comprising 100 upregulated 105 downregulated group compared group. Enrichment indicated significant enrichment Gene Ontology terms associated mammalian oogenesis, negative regulation neurotransmitter secretion, reproductive developmental processes, hormone-mediated signaling pathways, hormone secretion. Kyoto Encyclopedia Genes Genomes (KEGG) highlighted oxytocin pathway, GnRH oocyte maturation. low- contains (circRNAs), including chicirc_063269, chicirc_097731, chicirc_017440, chicirc_049641, chicirc_008429, chicirc_145057, chicirc_030156, chicirc_109497, chicirc_176754, chicirc_193363. have by modulating release serum hormones hypothalamus. A circRNA-miRNA constructed, which determined miR-135a, miR-188-3p, miR-101-3p, miR-128-3p may interact thereby regulating capacity through hypothalamic-pituitary-gonadal axis. results study enhance our knowledge molecular mechanisms regulate at hypothalamic level.

Language: Английский

Citations

0
Genetic Diversity and Selection Signatures of Lvliang Black Goat Using Genome-Wide SNP Data DOI Creative Commons
Ke Cai,

Wannian Wang,

Xu Wang

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(21), P. 3154 - 3154

Published: Nov. 3, 2024

Lvliang black goat (LBG) is an excellent local breed resource in China that known for its fur, meat quality, and strong adaptability. Studying the genetic mechanism germplasm characteristics of LBG can provide theoretical practical basis protection resources this help implement conservation breeding. In study, diversity population was evaluated using whole-genome SNP data. It found had a high low degree inbreeding. According to clustering results male goats relationship between individuals, divided into 13 families. Then, through structure analysis, it close with Nanjiang Qinggoda populations, they may have same ancestors. The has retained some ancient special integrates foreign gene flow. Through four selection signal analyses, we detected multiple candidate genes related economic traits (CFL2, SCD, NLRP14, etc.) adaptability (C4BPA, FUT8, PRNP, population. addition, comparative analysis three commercial breeds (Saanen goat, Boer Angora goat) also physical (ERG, NRG3, EDN3, etc.). Finally, performed functional enrichment on these explored their mechanisms. This study provides important data support breeding new perspective enriching populations.

Language: Английский

Citations

0
Investigation of genetic diversity in the loach Misgurnus anguillicaudatus revealed by whole-genome resequencing DOI Creative Commons
Xiaojuan Cui,

Jimin Deng,

Yifei Zhang

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Nov. 21, 2024

The loach (Misgurnus anguillicaudatus) is a significant freshwater economic fish in China, renowned for its tender meat, delicious taste, and high nutritional value. It widely distributed across the country, except western plateau. However, currently at risk of population decline degradation wildlife resources. Research on genetic diversity provides crucial foundation conservation development these To enhance protection utilization wild germplasm resources, we analyzed structure 60 populations from Xiangtan City (XT), Shaoyang (SY), Yueyang (XY) Hunan, Guilin (GL) Guiping (GP) Guangxi, Wuhan (WH) Hubei. Additionally, mapped DNA fingerprints loaches using 12 high-quality SNP sites. Whole genome resequencing (WGRS) was conducted six regions, yielding total 1047.17 Gb raw data 1046.98 clean data. From this 2,812,906 single nucleotide polymorphisms (SNPs) were identified, which 10,022 core SNPs selected to analyze structure, used assess fingerprint. analysis revealed that samples could be grouped into three clusters: Cluster A comprised XT, SY, XY groups; B included WH group; C consisted GL GP groups. mean nucleic acid (Pi), heterozygosity (Ho), expected (He) markers 0.130, 0.140, 0.123, respectively. average inbreeding coefficient 0.552, indicating levels inbreeding. an effective high-throughput approach identifying information describing relationships within genus. fingerprinting based marker technology can accurately variation natural populations, making it valuable tool strain identification. Our findings provide scientific basis conservation, development, optimal breeding native resources contribute expanding database populations.

Language: Английский

Citations

0