MICROBIAL DEGRADATION OF LIGNOCELLULOSE IN NATURAL AND ENGINEERED SYSTEMS – FROM THE SMALLEST TO THE BIGGEST BIOREACTOR DOI Open Access
Xavier Goux, Tong Liu, Maria Westerholm

et al.

Published: July 14, 2023

This chapter compares the lignocellulose degradation strategies of two model systems, where word "degradation" is defined as a hydrolysis (i.e. depolymerization) and further conversion through fermentation released products. The first an engineered microbe-driven process —anaerobic digestion. second shaped by nature-ingenuity — lignocellulose-degrading animal gut, with special focus given here to insect and, in particular, termite gut system. We will introduce context industrial biomass processing describe main paths for focusing on microbes their hydrolytic strategies. Second, we compare systems at different organizational levels, starting from system design, going microbial communities, ending carbohydrate-active enzymes involved plant degradation. massive development extensive use next generation sequencing technologies last decades has allowed in-depth insights into structure, diversity, dynamics, functioning these until recently unexplored misunderstood lignocellulolytic systems. With this knowledge, result billions years evolution could help us develop future applications energy molecules interest. To finish chapter, go beyond "standard" utilization highlight current trends challenges developments.

Language: Английский

Hepatincolaceae (Alphaproteobacteria) are Distinct From Holosporales and Independently Evolved to Associate With Ecdysozoa DOI Creative Commons
Michele Castelli, Leandro Gammuto, Diona Podushkina

et al.

Environmental Microbiology, Journal Year: 2025, Volume and Issue: 27(1)

Published: Jan. 1, 2025

ABSTRACT The Hepatincolaceae ( Alphaproteobacteria ) are a group of bacteria that inhabit the gut arthropods and other ecdysozoans, associating extracellularly with microvilli. Previous phylogenetic studies, primarily single‐gene analyses, suggested their relationship to Holosporales , which includes intracellular in protist hosts. However, genomics is still its early stages. In this study, number available genomes was increased examine evolutionary functional characteristics. It found previous grouping incorrect due sequence compositional biases form an independent branch within . This led reinterpretation features, proposing new scenario involves adaptation host association compared distinct specificities. exhibit greater nutritional flexibility, utilising various molecules thriving anaerobic conditions. they have less complex mechanism for modulating interactions, likely direct than those bacteria. addition, representatives show several lineage‐specific traits related differences species life

Language: Английский

Citations

1
Unveiling lignocellulolytic potential: a genomic exploration of bacterial lineages within the termite gut DOI Creative Commons
João Felipe Moreira Salgado, Vincent Hervé,

Manuel A. G. Vera

et al.

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: Oct. 15, 2024

The microbial landscape within termite guts varies across families. gut microbiota of lower termites (LT) is dominated by cellulolytic flagellates that sequester wood particles in their digestive vacuoles, whereas the flagellate-free higher (HT), activity has been attributed to fiber-associated bacteria. However, little known about role individual lineages fiber digestion, particularly LT.

Language: Английский

Citations

8
Insect–microbe interactions and their influence on organisms and ecosystems DOI Creative Commons
Jocelyn R. Holt, Nathalia Cavichiolli de Oliveira, Raúl F. Medina

et al.

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(7)

Published: July 1, 2024

Abstract Microorganisms are important associates of insect and arthropod species. Insect‐associated microbes, including bacteria, fungi, viruses, can drastically impact host physiology, ecology, fitness, while many microbes still have no known role. Over the past decade, we increased our knowledge taxonomic composition functional roles insect‐associated microbiomes viromes. There has been a more recent shift toward examining complexity microbial communities, how they vary in response to different factors (e.g., genome, strain, environment, time), consequences this variation for wider ecological community. We provide an overview insect–microbe interactions, variety associated functions, evolutionary ecology these relationships. explore influence environment interactive effects insects their across trophic levels. Additionally, discuss potential subsequent synergistic reciprocal impacts on microbiomes, communities. Lastly, some avenues future insect‐microbe interactions that include modification existing symbionts as well construction synthetic

Language: Английский

Citations

5
Genome‐Scale Community Model‐Guided Development of Bacterial Coculture for Lignocellulose Bioconversion DOI Creative Commons
Pritam Kundu, Amit Ghosh

Biotechnology and Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 5, 2025

Microbial communities have shown promising potential in degrading complex biopolymers, producing value-added products through collaborative metabolic functionality. Hence, developing synthetic microbial consortia has become a predominant technique for various biotechnological applications. However, diverse entities consortium can engage distinct biochemical interactions that pose challenges mutualistic communities. Therefore, systems-level understanding of the inter-microbial interactions, growth compatibility, and synergisms is essential effective consortia. This study demonstrated genome-scale community modeling approach to assess interaction pattern screen metabolically compatible bacterial pairs designing lignocellulolytic coculture system. Here, we investigated pairwise among six termite gut isolates by implementing flux-based parameters, i.e., support index (PGSI) assistance (PMA). Assessment PGSI PMA helps nine beneficial were validated experiment with lignocellulosic substrates. For cocultured pairs, experimentally measured enzymatic (DES) showed good coherence model-derived compatibility (PMA), which explains fidelity silico predictions. The highest degree been observed C. denverensis P3 Brevibacterium sp P5 coculture, where total cellulase activity increased 53%. assessment select best system enhanced parameters (PGSI PMA) proposed strategy will help optimize composition microcosms bioremediation, bioengineering, biomedical

Language: Английский

Citations

0
Thermally modified wood: assessing the impact of weathering on mechanical strength and exposure to subterranean termites DOI
Delfina Godinho, Ana Lourenço, Solange de Oliveira Araújo

et al.

European Journal of Wood and Wood Products, Journal Year: 2025, Volume and Issue: 83(1)

Published: Jan. 15, 2025

Language: Английский

Citations

0
Differences in Microbial Community Structure Determine the Functional Specialization of Gut Segments of Ligia exotica DOI Creative Commons

Zhao-Zhe Xin,

Ke Ma, Yonglin Che

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 808 - 808

Published: April 2, 2025

Ligia feed on seashore algae and remove organic debris from the coastal zone, thereby playing an important role in intertidal ecosystem. Nevertheless, specific roles of distinct gut segments transit remain unclear. We collected identified exotica specimens coast Aoshanwei, Qingdao, Shandong Province, analyzed their foreguts hindguts for 16S rRNA, metagenomics, metabolomics, proteomics. The concentrations common metabolites, NO3−-N NH4+-N, contents C N were measured. decreased abundances dominant phyla Cyanobacteria but increased Proteobacteria, Firmicutes, Actinobacteria, Planctomycetes Bacteroidetes remained relatively constant. foregut microbiota is involved carbohydrates amino acids metabolism, as well decomposition polysaccharides. hindgut performs a variety functions, including carbohydrate acid fermentation, cell motility, intracellular transport, secretion, vesicular translocation, polysaccharides, disaccharides, oligosaccharides. results omics analyses molecular experiments demonstrated that metabolic processes involving are more active foregut, whereas absorption, assimilation hindgut. Taken together, differences microbial community structure determine functional specialization different segments, i.e., appears to be primary site digesting food, while further absorbs nutrients then excretes them.

Language: Английский

Citations

0
Exploring the termite gut as a hub of industrially important microbes and enzymes for biofuel production DOI Creative Commons
Emmanuel Tapiwa Sero, Ayodeji Amobonye, Prashant Bhagwat

et al.

Biomass and Bioenergy, Journal Year: 2025, Volume and Issue: 199, P. 107899 - 107899

Published: April 16, 2025

Language: Английский

Citations

0
Biotechnological utilization: the potential role of the termite gut symbiotic microbiome DOI Creative Commons
Ben Jesuorsemwen Enagbonma,

Rorisang Mmushi,

Olubukola Oluranti Babalola

et al.

Symbiosis, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

Language: Английский

Citations

0
Metagenomics analysis of bacterial community structure from wood- and soil-feeding termites: metabolic pathways and functional structures toward the degradation of lignocellulose and recalcitrant compounds DOI Creative Commons
Rongrong Xie,

Blessing Danso,

Jianzhong Sun

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 15, 2024

Some essential information on gut bacterial profiles and their unique contributions to food digestion in wood-feeding termites (WFT) soil-feeding (SFT) is still inadequate. The feeding type of hypothesized influence composition its functionality degrading lignocellulose or other organic chemicals. This could potentially provide alternative approaches for the degradation some recalcitrant environmental Therefore, metagenomic analysis can be employed examine functional symbionts WFT SFT. Based 16S rRNA gene sequences WFT, Microcerotermes sp., SFT, Pericapritermes nitobei , findings revealed a total 26 major phyla, with 18 phyla commonly represented both termites, albeit varying abundances. Spirochaetes dominated sp. at 55%, followed by Fibrobacters, while Firmicutes bacteria P. 95%, Actinobacteria coming second 2%. Furthermore, Shannon phylogenetic tree diversity indices, as well observed operational taxonomic units Chao 1 richness were all found higher than SFT deduced from alpha analysis. principal coordinate analysis, exhibited significant distance dissimilarity between symbionts. results showed that differed significantly Tax4Fun evaluated functions, revealing predominance carbohydrate metabolism, amino acid metabolism energy termites. implicated inhabiting guts actively involved compounds.

Language: Английский

Citations

3
Biorefinery and Bioremediation Strategies for Efficient Management of Recalcitrant Pollutants Using Termites as an Obscure yet Promising Source of Bacterial Gut Symbionts: A Review DOI Creative Commons
Rongrong Xie,

Blessing Danso,

Jianzhong Sun

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(11), P. 908 - 908

Published: Nov. 20, 2024

Lignocellulosic biomass (LCB) in the form of agricultural, forestry, and agro-industrial wastes is globally generated large volumes every year. The chemical components LCB render them a substrate valuable for biofuel production. It hard to dissolve resources production because lignin, cellulose, hemicellulose parts stick together rigidly. This makes structure complex, hierarchical, resistant. Owing these restrictions, junk waste has recently become significant worldwide environmental problem resulting from inefficient disposal techniques increased persistence. In addition, burning waste, such as paddy straws, widespread practice that causes considerable air pollution endangers environment human existence. Besides increasing industrialization resulted billions tons dyeing wastewater several industries, including textiles, pharmaceuticals, tanneries, food processing units. massive use synthetic dyes various industries can be detrimental due recalcitrant aromatic dyes, similar polymeric phenol lignin structure, their persistent color. Synthetic have been described possessing carcinogenic toxic properties could harmful public health. Environmental emanating great concern should carefully handled mitigate its catastrophic effects. An effective strategy curtail problems learn analogous systems nature, termites, where woody lignocellulose digested by wood-feeding termites humus-recalcitrant compounds are decomposed soil-feeding termites. termite gut system acts unique bioresource consisting distinct bacterial species valued lignocellulosic materials degradation which integrated into modern biorefineries bioremediation applications treatment wastewaters help resolve issues arising wastewaters. review paper provides new efficient management pollutants exploring potential application bacteria biorefinery processing.

Language: Английский

Citations

3