Expression and Immune Response Profiles in Nile Tilapia (Oreochromis niloticus) and European Sea Bass (Dicentrarchus labrax) During Pathogen Challenge and Infection DOI Open Access
Ahmed A. Saleh,

A. Mohamed,

Shaaban S. Elnesr

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12829 - 12829

Published: Nov. 28, 2024

Nile tilapia (Oreochromis niloticus) and European sea bass (Dicentrarchus labrax) are economically significant species in Mediterranean countries, serving essential roles the aquaculture industry due to high market demand nutritional value. They experience substantial losses from bacterial pathogens Vibrio anguillarum Streptococcus iniae, particularly at onset of summer season. The immune mechanisms involved fish infections by V. S. iniae remain poorly understood. This study investigated their impact through experiments with control anguillarum- iniae-infected groups for each species. Blood samples were collected 1, 3, 7 days post injection assess biochemical immunological parameters, including enzyme activities (AST ALT), oxidative markers (SOD, GPX, CAT, MDA), leukocyte counts. Further analyses included phagocyte activity, lysozyme IgM levels, complement C3 C4 levels. Muscle tissues sampled mRNA expression levels 18 immune-relevant genes. focus was on cytokines immune-related genes, pro-inflammatory (TNF-α, TNF-β, IL-2, IL-6, IL-8, IL-12, IFN-γ), major histocompatibility complex components (MHC-IIα MHC-IIβ), cytokine receptors (CXCL-10 CD4-L2), antimicrobial peptides (Pleurocidin β-defensin), regulatory (Thymosin β12, Leap 2, Lysozyme g), Galectins (Galectin-8 Galectin-9). β-actin used as housekeeping gene normalization. Significant species-specific responses observed N. Tilapia E. Sea Bass when infected highlighting differences biochemical, immune, profiles. Notably, Tilapia, AST significantly increased day during infection, reaching 45.00 ± 3.00 (p < 0.05), indicating late-stage acute stress or tissue damage. Conversely, exhibited a rise ALT group, peaking 33.5 3.20 suggesting liver distress systemic inflammatory response. On front, showed increases respiratory burst activity 1 both pathogens, values 0.28 0.03 0.25 0.02 robust initial activation. Finally, analysis revealed pronounced peak TNF-α infection fold change 6.120, strong response strategy. Understanding these provides critical insights enhancing disease management productivity operations.

Language: Английский

Exploring the impact of nano-Se and nano-clay feed supplements on interleukin genes, immunity and growth rate in European Sea Bass (Dicentrarchus labrax) DOI Creative Commons

Asmaa A. Khaled,

Amany M. Shabaan,

Saad Hammad

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 1, 2024

Abstract This study aimed to investigate the effects of adding Nano-Selenium (NSe) and Nano-clay (NC) as feed supplements on European Sea Bass ( Dicentrarchus labrax ). Two separate experiments were conducted, one with NC other NSe. Each experiment consisted four sub-groups varying concentrations or The expression levels five immune-related genes TNF-α , TNF-β IL-2 IL-6 IL-12 ) measured using Real-time Quantitative PCR (Rt-PCR) Assay. results showed an increase in interleukins pro-inflammatory cytokines after exposure NSe . gene was significantly higher both 1 mg 10 highest 5 concentration NC. for NC, mg, NSe, led p < 0.05) compared control. Similar trends observed Understanding impact these expression, growth rate, biochemical indices, antioxidant status can provide valuable insights into potential applications Bass.

Language: Английский

Citations

8
Molecular Mechanisms of Cold Stress Response in Cotton: Transcriptional Reprogramming and Genetic Strategies for Tolerance DOI
Washu Dev, Fahmida Sultana, Hongge Li

et al.

Plant Science, Journal Year: 2025, Volume and Issue: unknown, P. 112390 - 112390

Published: Jan. 1, 2025

Language: Английский

Citations

0
Differential responses of the intestine and liver transcriptome to high levels of plant proteins in diets for large yellow croaker (Larimichthys crocea) DOI Creative Commons
Qiaozhen Ke,

Yin Li,

Huai-Kai Weng

et al.

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: March 5, 2025

Large yellow croaker is an economically important carnivorous marine aquaculture fish in China with high protein requirements. Current meal - based feeds face issues like cost and resource depletion, while plant sources have potential but also controversies. To explore this, a 120 day feeding trial was conducted standard commercial feed (CF) modified (PF) where 70% of replaced by protein. Results showed no significant growth performance differences between the two groups. Transcriptome analysis identified 557 308 differentially expressed genes liver intestine respectively. GO KEGG enrichment analyses indicated their association immune response, lipid metabolism, signal transduction. Five key related to metabolism regulation were found. These findings underscore integrating into diets, which could significantly enhance sustainable practices global reducing reliance on meal. Emphasizing this transition crucial for fostering environmental sustainability supporting future aquaculture.

Language: Английский

Citations

0
Sustained cold resistance after recovery from cold pretreatment in large yellow croaker: Physiological, histological, ultrastructural, and molecular insights in the highly sensitive gill DOI
Xiaolong Yin, Xian Xu, Haibo Qiu

et al.

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742694 - 742694

Published: May 1, 2025

Language: Английский

Citations

0
Genome-wide identification of gap junction gene family and their expression profiles under low temperature stress in noble scallop Chlamys nobilis DOI
Changping Xu,

Xuanbing Wu,

Jiale Qiu

et al.

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2024, Volume and Issue: 52, P. 101310 - 101310

Published: Aug. 12, 2024

Language: Английский

Citations

2
Construction of a high-density genetic linkage map and QTL mapping of growth and cold tolerance traits in Takifugu fasciatus DOI Creative Commons
Ying Zhang, Jie Li,

Peng Chu

et al.

BMC Genomics, Journal Year: 2023, Volume and Issue: 24(1)

Published: Oct. 27, 2023

Takifugu fasciatus is an aquaculture species with high economic value. In recent years, problems such as environmental pollution and inbreeding have caused a serious decline in T. germplasm resources. this study, high-density genetic linkage map was constructed by whole-genome resequencing. The consists of 4891 bin markers distributed across 22 groups (LGs), total coverage 2381.353 cM mean density 0.535 cM. Quantitative trait locus (QTL) localization analysis showed that 19 QTLs associated growth traits the genome-wide significance threshold range, on 11 LGs. addition, cold tolerance were identified, each scattered different LG. Furthermore, we used QTL to screen out three candidate genes (IGF1, IGF2, ADGRB) related fasciatus. Meanwhile, screened (HSP90, HSP70, HMGB1) tolerance. Our study can provide theoretical basis for selection breeding cold-tolerant or fast-growing

Language: Английский

Citations

6
Effects of saline-alkaline stress on metabolomics profiles, biochemical parameters, and liver histopathology in large yellow croaker (Larimichthys crocea) DOI
Fengfang Zhou,

Mengyang Chang,

Lan Yan

et al.

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2024, Volume and Issue: 52, P. 101343 - 101343

Published: Oct. 14, 2024

Language: Английский

Citations

1
Integrated Analysis of Transcriptome and Metabolome in the Brain After Cold Stress of Red Tilapia During Overwintering DOI Open Access
Chenxi Zhu, Haoran Yang, Wenbin Zhu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13372 - 13372

Published: Dec. 13, 2024

Cold stress during overwintering is considered a bottleneck problem limiting the development of red tilapia (Oreochromis spp.) industry, and regulation mechanism currently not well understood. In this study, fish (initial weight: 72.71 ± 1.32 g) were divided into cold group (cold) control (normal) group. group, water temperature was maintained at 20 °C, which basically consistent with in greenhouses local areas. decreased from °C to 8 by 2 per day experiment. At end experiment, levels serum urea nitrogen, glucose, norepinephrine, alkaline phosphatase, total bilirubin, cholesterol changed significantly compared that (P < 0.05). Then transcriptome sequencing LC–MS metabolome brain tissue further employed obtain mRNA metabolite datasets. We found FoxO signaling pathway ABC transporters played an important role transcriptome–metabolome association analysis. pathway, differentially expressed genes related cell cycle regulation, apoptosis immune-regulation, oxidative resistance DNA repair. ATP-binding cassette (ABC) subfamily abca, abcb, abcc gene expression levels, deoxycytidine, L-lysine, L-glutamic acid, L-threonine, ornithine, uridine contents changed. Our results suggested may promote through pathway. The respond regulating amino acid metabolism. provided comprehensive understanding overwintering, will facilitate breeding new cold-resistant varieties future.

Language: Английский

Citations

1
Expression and Immune Response Profiles in Nile Tilapia (Oreochromis niloticus) and European Sea Bass (Dicentrarchus labrax) During Pathogen Challenge and Infection DOI Open Access
Ahmed A. Saleh,

A. Mohamed,

Shaaban S. Elnesr

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12829 - 12829

Published: Nov. 28, 2024

Nile tilapia (Oreochromis niloticus) and European sea bass (Dicentrarchus labrax) are economically significant species in Mediterranean countries, serving essential roles the aquaculture industry due to high market demand nutritional value. They experience substantial losses from bacterial pathogens Vibrio anguillarum Streptococcus iniae, particularly at onset of summer season. The immune mechanisms involved fish infections by V. S. iniae remain poorly understood. This study investigated their impact through experiments with control anguillarum- iniae-infected groups for each species. Blood samples were collected 1, 3, 7 days post injection assess biochemical immunological parameters, including enzyme activities (AST ALT), oxidative markers (SOD, GPX, CAT, MDA), leukocyte counts. Further analyses included phagocyte activity, lysozyme IgM levels, complement C3 C4 levels. Muscle tissues sampled mRNA expression levels 18 immune-relevant genes. focus was on cytokines immune-related genes, pro-inflammatory (TNF-α, TNF-β, IL-2, IL-6, IL-8, IL-12, IFN-γ), major histocompatibility complex components (MHC-IIα MHC-IIβ), cytokine receptors (CXCL-10 CD4-L2), antimicrobial peptides (Pleurocidin β-defensin), regulatory (Thymosin β12, Leap 2, Lysozyme g), Galectins (Galectin-8 Galectin-9). β-actin used as housekeeping gene normalization. Significant species-specific responses observed N. Tilapia E. Sea Bass when infected highlighting differences biochemical, immune, profiles. Notably, Tilapia, AST significantly increased day during infection, reaching 45.00 ± 3.00 (p < 0.05), indicating late-stage acute stress or tissue damage. Conversely, exhibited a rise ALT group, peaking 33.5 3.20 suggesting liver distress systemic inflammatory response. On front, showed increases respiratory burst activity 1 both pathogens, values 0.28 0.03 0.25 0.02 robust initial activation. Finally, analysis revealed pronounced peak TNF-α infection fold change 6.120, strong response strategy. Understanding these provides critical insights enhancing disease management productivity operations.

Language: Английский

Citations

0